Adaptive Wavefront Generation and Correction for Super-high Resolution Microscopy

描述（由申请人提供）：本项目的目标是将自适应光学（AO）方法应用于受激发射耗尽（STED）显微镜（AO-STED），以便自适应地保持STED光束的波前，以确保组织中的超高空间分辨率。在AO的帮助下，我们还将开发在轴向和横向方向上具有改进的空间分辨率的超分辨率显微镜（AO 3D-STED）。本申请的目标是开发一种使能方法，该方法可以在深层组织成像中有效地实现衍射无限分辨率;这里的“有效”是指以最小的样本曝光量实现超高分辨率。拟议的机制将建立作为一个除了现有的自制STED共焦/双光子激光扫描显微镜。我们的目标是创建一个强大的先进的显微镜，可以实际和常规用于成像的各种类型的组织，而不妥协的分辨率。该项目的成功完成将促进超高分辨率成像在生物医学研究中的广泛应用。 公共卫生相关性（由申请人提供）：拟议项目如果成功完成，将产生一种先进的显微成像系统，该系统将在具有不同光学特性、厚度和维护条件的组织中保持亚衍射限制的空间分辨率。该系统将有益于需要高分辨率和活体组织成像的生物医学研究领域。该研究项目的成功完成将使生物学家和医学研究人员广泛使用显微镜。

DESCRIPTION (provided by applicant): The goal of this project is to implement adaptive optics (AO) approach to stimulated emission depletion (STED) microscopy (AO-STED) so that the wavefront of the STED beam can be adaptively maintain to ensure the super-high spatial resolution in tissues. With the assistance of AO we will also develop the super-resolution microscopy with improved spatial resolution in both axial and lateral directions (AO3D-STED). The goal of this application is to develop an enabling method that can achieve a diffraction-unlimited resolution efficiently in deep tissue imaging; "efficiently" here means to achieve super-high resolution with minimum light exposure to the specimen. The proposed mechanism will be built as an addition to an existing home-built STED confocal/two-photon laser scanning microscope. We aim to create a robust advanced microscope that can be practically and routinely used for imaging various types of tissues without compromised resolution. The successful completion of this project will facilitate the widespread applications of super-high resolution imaging in biomedical research. PUBLIC HEALTH RELEVANCE (provided by applicant): The proposed project, if successfully completed, leads to an advanced microscopy imaging system that will maintain sub-diffraction-limited spatial resolution in tissues with different optical properties, thickness nd maintaining conditions. This system will benefit the biomedical research field in which high resolution and live tissue imaging are needed. The successful completion of this research project will make the microscope widely accessible to biologists and medical researchers.