Adaptive Wavefront Generation and Correction for Super-high Resolution Microscopy

超高分辨率显微镜的自适应波前生成和校正

• 批准号: 8352334
• 负责人: Tong Ye
• 金额: $ 17.24万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8352334
• 关键词: Animals; Biological; Biomedical Research; Development; Ensure; Exposure to; Fluorescence; Funding; Generations; Goals; Histocompatibility Testing; Home environment; Image; Lasers; Lateral; Life; Light; Location; Medical; Methods; Microscope; Microscopy; Optics; Photons; Property; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Route; Sampling; Scanning; Shapes; Slice; Source; Specimen; System; Thick; Tissues; United States National Institutes of Health; Vertebral column; adaptive optics; base; brain morphology; coping; diffraction of light; imaging modality; improved; light microscopy; photoactivation; reconstruction; response; single molecule; two-photon

DESCRIPTION (provided by applicant): The goal of this project is to implement adaptive optics (AO) approach to stimulated emission depletion (STED) microscopy (AO-STED) so that the wavefront of the STED beam can be adaptively maintain to ensure the super-high spatial resolution in tissues. With the assistance of AO we will also develop the super-resolution microscopy with improved spatial resolution in both axial and lateral directions (AO3D-STED). The goal of this application is to develop an enabling method that can achieve a diffraction-unlimited resolution efficiently in deep tissue imaging; "efficiently" here means to achieve super-high resolution with minimum light exposure to the specimen. The proposed mechanism will be built as an addition to an existing home-built STED confocal/two-photon laser scanning microscope. We aim to create a robust advanced microscope that can be practically and routinely used for imaging various types of tissues without compromised resolution. The successful completion of this project will facilitate the widespread applications of super-high resolution imaging in biomedical research. PUBLIC HEALTH RELEVANCE (provided by applicant): The proposed project, if successfully completed, leads to an advanced microscopy imaging system that will maintain sub-diffraction-limited spatial resolution in tissues with different optical properties, thickness nd maintaining conditions. This system will benefit the biomedical research field in which high resolution and live tissue imaging are needed. The successful completion of this research project will make the microscope widely accessible to biologists and medical researchers.

描述（由申请人提供）：本项目的目标是将自适应光学（AO）方法应用于受激发射耗尽显微镜（AO-STED），使受激发射耗尽显微镜（AO-STED）光束的波前能够自适应维持，以确保组织中的超高空间分辨率。在AO的帮助下，我们还将开发具有提高轴向和横向空间分辨率的超分辨率显微镜（AO3D-STED）。本应用程序的目标是开发一种使能方法，可以在深层组织成像中有效地实现衍射无限分辨率；这里的“高效”是指以最小的光照量达到超高分辨率。所提出的机制将作为现有的国产STED共聚焦/双光子激光扫描显微镜的补充。我们的目标是创造一种强大的先进显微镜，可以实际和常规地用于成像各种类型的组织而不影响分辨率。该项目的成功完成将促进超高分辨率成像在生物医学研究中的广泛应用。