Development of Efficient table-top laser-produced plasma sources for Water-Window
开发用于水窗的高效台式激光产生等离子体源
基本信息
- 批准号:8206546
- 负责人:
- 金额:$ 18.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2010
- 资助国家:美国
- 起止时间:2010-01-01 至 2012-12-31
- 项目状态:已结题
- 来源:
- 关键词:AreaArtsBiologicalBiomedical ResearchCarbonCellsCellular biologyCharacteristicsCouplingDevelopmentEnsureExperimental ModelsFreezingGoalsImageInternetLaboratory ResearchLasersLeadLightLightingLipidsMedicalMedical ResearchMicroscopeMicroscopyOpticsOxygenPhotonsPhysicsPhysiologic pulsePlasmaPreparationProcessProteinsRadiationResearch PersonnelResolutionRiskRoentgen RaysRoleSamplingSourceSpecimenSpottingsStructureSynchrotronsSystemTechniquesTestingTimeTissuesTransmission Electron MicroscopyWaterWidthWorkabsorptionbasebiological researchcomputerized toolsdensitydesignimprovedinnovationinstrumentlight scatteringnovelpublic health relevanceresearch facilityresearch studysimulationsource guidessynchrotron radiationtomographytooltransmission process
项目摘要
DESCRIPTION (provided by applicant): Project Summary Imaging of biological specimens must be performed with high spatial and temporal resolution, while at the same time avoiding damage to the sample. Present techniques, such as optical microscopy and transmission electron microscopy (TEM), are valuable and widely-used tools in biomedical studies/research, but have limitations. For example, the resolution of optical microscopy is not as high as that which can be obtained using short-wavelength light. For TEM, sample preparation is tedious and risks altering the sample structure. A microscope operating in the water-window wavelength region (= 2.3 - 4.4 nm) provides high resolution as well as a large depth of focus, which enables performing micro-tomography. Microscopy at this wavelength provides a strong natural contrast between water and carbon-rich tissues such as proteins and lipids. Very recently water-window microscopes using synchrotron radiation showed great potential for high resolution three-dimensional tomographic images of frozen cells. However, the limited accessibility and large expense associated with synchrotron-based sources significantly limits the role of WW microscopy in biomedical research. The full potential of WW microscopy will only be realized if it becomes widely accessible to a broad variety of medical laboratories and research facilities. In this project, we propose to develop an efficient, table-top water-window radiation source for transmission X-ray microscope (TXM) based on laser- produced plasma (LPP) light source. LPP-based WW-TXM systems require a soft X-ray source that is efficient, stable and debris free. LPP sources are viable light sources for WW-TXM, but up to now have been limited by their relatively low poor efficiency in converting laser energy into useable soft X-ray light. Low efficiency of LPP sources leads to longer exposure time for imaging of a biological sample. It implies efficient or high conversion efficiency (CE, conversion from laser energy to WW radiation) LPP sources are prerequisite for using them as a source for WWW-TXM. CE of a LPP depends on numerous laser and target characteristics and it is very challenging to identify optimum parameters for obtaining optimum CE. This can be attained only through a combination of modeling and experimental work. We will use our state-of-the-art radiation and atomic physics simulation tools to identify laser and source characteristics that produce highly efficient soft X- ray sources, and guide the experimental campaign for developing novel light sources. This project will lead to affordable, table-top soft X-ray light source in the water-window region that will be of significant benefit to the fields of biomedical research and cell biology.
PUBLIC HEALTH RELEVANCE (provided by applicant): Project Narrative Images of biological cells are fundamental in many areas of medical and biological research. We propose to develop an efficient table-top light source instrument for water-window Transmission X-ray microscope (WW-TXM). Compact WW-TXM can provide highly detailed, high contrast images of carbon based structures in biological cells, while minimizing the impact of the water based growing medium, will ensure that this exciting technique is readily available to the maximum number of researchers.
描述(由申请人提供):项目摘要生物样本的成像必须以高空间和时间分辨率进行,同时避免对样本造成损坏。目前的技术,如光学显微镜和透射电子显微镜(TEM),是有价值的和广泛使用的工具,在生物医学研究/研究,但有局限性。例如,光学显微镜的分辨率不如使用短波长光所能获得的分辨率高。对于TEM,样品制备是繁琐的,并且有改变样品结构的风险。在水窗波长区域(= 2.3 - 4.4nm)中操作的显微镜提供高分辨率以及大的焦深,这使得能够执行微断层摄影。在这个波长下的显微镜检查在水和富含碳的组织(如蛋白质和脂质)之间提供了强烈的自然对比。最近,水窗显微镜使用同步辐射显示了巨大的潜力,高分辨率的三维断层图像的冷冻细胞。然而,有限的可及性和与同步加速器为基础的源相关的大量费用显着限制了WW显微镜在生物医学研究中的作用。WW显微镜的全部潜力只有在广泛的医学实验室和研究设施中才能实现。在本计画中,我们提出以雷射电浆光源为基础,发展一种高效率的桌上型水窗辐射源,以应用于穿透式X光显微镜。基于LPP的WW-TXM系统需要高效、稳定且无碎片的软X射线源。 LPP源是用于WW-TXM的可行光源,但是到目前为止,LPP源在将激光能量转换成可用的软X射线光方面受到其相对低的效率的限制。LPP源的低效率导致用于生物样品成像的较长曝光时间。这意味着高效或高转换效率(CE,从激光能量到WW辐射的转换)LPP源是将它们用作WWW-TXM源的先决条件。LPP的CE取决于许多激光和目标特性,并且识别用于获得最佳CE的最佳参数是非常具有挑战性的。这只能通过建模和实验工作相结合来实现。我们将使用我们最先进的辐射和原子物理模拟工具来识别产生高效软X射线源的激光和源特性,并指导开发新型光源的实验活动。该项目将在水窗区域产生负担得起的桌面软X射线光源,这将对生物医学研究和细胞生物学领域产生重大影响。
公共卫生相关性(由申请人提供):生物细胞的项目叙述图像在医学和生物学研究的许多领域都是基础。我们提出了一种用于水窗透射X射线显微镜(WW-TXM)的高效台式光源装置。紧凑型WW-TXM可以提供生物细胞中碳基结构的高度详细,高对比度图像,同时最大限度地减少水基生长介质的影响,将确保这种令人兴奋的技术随时可供最大数量的研究人员使用。
项目成果
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Sivanandan S Harilal其他文献
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{{ truncateString('Sivanandan S Harilal', 18)}}的其他基金
Development of Efficient table-top laser-produced plasma sources for Water-Window
开发用于水窗的高效台式激光产生等离子体源
- 批准号:
8009819 - 财政年份:2010
- 资助金额:
$ 18.29万 - 项目类别:
Development of Efficient table-top laser-produced plasma sources for Water-Window
开发用于水窗的高效台式激光产生等离子体源
- 批准号:
7762372 - 财政年份:2010
- 资助金额:
$ 18.29万 - 项目类别:
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