权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Anti-Inflammatory Approaches

抗炎方法

基本信息

批准号：
8432089
负责人：
TERRY B. STROM
金额：
$ 16.64万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-08-01 至 2017-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8432089
关键词：
Abate Acute-Phase Proteins Allogenic Allografting Anti-Inflammatory Agents Anti-inflammatory Base Sequence Biological Assay Biopsy Blood Blood Cells Blood specimen Cells Chimerism Clinical Trials Commit Custom Cytoprotective Agent Data Defect Diagnosis Equilibrium Frequencies Genes Genetic Transcription Goals Housing Human Immunity Inflammation Inflammatory Instruction Kidney Logic Macaca fascicularis Macaca mulatta Methods Modeling Molecular Profiling Monitor Monkeys Organ Transplantation Pan Genus Patients Phenotype Polymerase Chain Reaction Protein C Inhibitor Proteins Reagent Regulatory T-Lymphocyte Replacement Therapy Resistance Reverse Transcriptase Polymerase Chain Reaction Role Serine Protease Services T-Lymphocyte T-Lymphocyte Subsets Techniques Time Tissue Sample Tissues Transcript Translating Transplant Recipients Transplantation United States National Institutes of Health Work allograft rejection base cytokine design in vivo Model interest islet man novel response

项目摘要

PROJECT SUMMARY (See instructions): Our recent data show that cynomolgus transplant recipients are resistant to the induction of chimerism and transplant tolerance until the pro-inflammatory state that arises in the peri-transplant period and continues for weeks to months thereafter abates. Projects 1 and 2 place heavy emphasis upon the role of inflammation in influencing the allograft response toward or away from transplant tolerance. In accordance with this logic, Core B will interact with all of the Project 1 and 2 in vivo models, by monitoring and characterizing the state of inflammation and immunity via QRT-PCR in the cynomolgus allograft recipients. Using a panel of custom made reagents crafted for use in cynomolgus monkeys and a powerful pre-amplification technique, we can assess transcription of over 65 genes in a small tissue sample. Going forward we will further develop a single cell PCR method to provide detailed information about the frequency of T cell subsets in recipient blood or tissues. The goal is to favorably tilt the balance of immunity toward allograft protection and tolerance by controlling adverse inflammation. T alphal antitrypsin (AAT), an acute phase reactant and serine protease blocker is a potential agent for achieving such balance. It has been given safely as replacement therapy to patients with the AAT gene defect. This protein possesses interesting anti-inflammatory and anti-thrombotic effects. It is a potent islet cytoprotectant and although lacking direct effects upon T cells promotes T cell tolerance. This and other novel agents will be characterized in Core B for use in the Project 1 and 2 models.

项目概述（见说明）：