New targets for antibiotics: self assembling ribonucleoprotein complexes

抗生素的新靶点：自组装核糖核蛋白复合物

• 批准号: 8310270
• 负责人: LOIS POLLACK
• 金额: $ 26.3万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-10 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8310270
• 关键词: Address; Antibiotic Resistance; Antibiotics; Bacteria; Binding; Binding Sites; Biological; Complex; Coupled; Development; Generations; Goals; Growth; Health; Human; In Vitro; Ions; Knowledge; Literature; Macromolecular Complexes; Measurement; Measures; Methods; Molecular Conformation; Molecular Machines; Neutrons; Pathway interactions; Pharmaceutical Preparations; Precursor RNA; Property; Protein Binding; Protein Biosynthesis; Protein Precursors; Proteins; RNA; RNA Conformation; RNA Precursors; RNA Processing; Reporting; Resolution; Ribonucleoproteins; Ribosomes; Staging; Structure; Time; Variant; Work; drug resistant bacteria; fight against; insight; interest; macromolecule; novel strategies; prevent; research study; resistant strain; self assembly; tool; weapons

Ribosomes are molecular machines that carry out an essential biological task: they synthesize proteins. Thus, bacterial ribosomes are logical targets for antibiotics. However, the emergence (and growth of) drug resistant bacteria poses a major threat to human health. New strategies for attacking resistant strains must be developed. Recent, breakthrough structural studies of the ribosome enable an entirely new approach to interfering with the ribosome: preventing its self assembly. Here, we propose to develop and apply a new experimental method for measuring structural changes accompanying the real time self-assembly of the ribosome or its subunits. If successful, this approach will enable identification of folding pathways or partially folded states that may be amenable to attack.

核糖体是一种分子机器， 任务：它们合成蛋白质。因此，细菌核糖体是 抗生素然而，耐药细菌的出现（和生长） 对人类健康构成重大威胁。新的抗攻击策略 必须开发菌株。最近，突破性的结构研究， 核糖体使一种全新的方法来干扰核糖体： 防止其自组装。在这里，我们建议开发和应用一种新的 测量伴随真实的结构变化的实验方法 核糖体或其亚基的时间自组装。如果成功，这种方法 将使得能够识别折叠路径或部分折叠状态， 易于攻击。