Probing dynamics of water in elastin by q-space imaging and multiple quantum NMR

通过 q 空间成像和多量子 NMR 探测弹性蛋白中水的动力学

• 批准号: 8206692
• 负责人: Gregory Boutis
• 金额: $ 27.2万
• 依托单位: BROOKLYN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2013-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8206692
• 关键词: Anisotropy; Atomic Force Microscopy; Behavior; Biomolecular Nuclear Magnetic Resonance; Biopolymers; Cell Nucleus; Cities; Collaborations; Communities; Coupled; Deuterium; Disease; Educational workshop; Elastic Fiber; Elasticity; Elastin; Elastin Fiber; Faculty; Fruit; Funding; Goals; Grant; Heart; Hydration status; Image; Institution; Investigation; Journals; Knowledge; Laboratories; Life; Magnetic Resonance; Magnetic Resonance Imaging; Measurement; Measures; Mechanics; Methods; Microscopy; Modeling; Molecular; Motion; Nature; New York; Nuclear; Nuclear Magnetic Resonance; Organism; Peer Review; Physiologic pulse; Play; Probability; Progress Reports; Proteins; Protons; Publishing; Research; Research Personnel; Resolution; Role; Sampling; Scheme; Signal Transduction; Sister; Slice; Staging; Stress; Structure; Structure-Activity Relationship; Surface; Surface Tension; Techniques; Temperature; Testing; Tissues; United States National Institutes of Health; Universities; Vertebral column; Water; Work; Writing; base; burden of illness; college; density; disability; experience; forging; fundamental research; meetings; member; novel; programs; quantum; radiofrequency; research study; skills; solid state; solid state nuclear magnetic resonance; symposium; theories

DESCRIPTION (provided by applicant): Elastin is the main protein of the elastic fiber found in various vertebrate tissues. The hydration level in this mainly hydrophobic protein is known to play an important role in its structure and function. Many diseases of various tissues, such as the heart, have been shown to be correlated to structural degradation of elastin. The broad objective of the proposed research effort is to investigate the role of water in the structure and function of elastin using novel strong pulse gradient q-space Nuclear Magnetic Resonance (NMR) imaging, spin counting NMR and deuterium double quantum filtered NMR studies. Q-space imaging is a well known scheme for measuring the probability of molecular displacement in a sample. This technique was successfully implemented in the previously funded project to measure the average surface- to-volume ratio of pores in elastin fibers. The technique will extended to measuring the surface tension of water confined in pores of elastin by mechanically stressing a sample. Double Quantum Filtered NMR on deuterium- hydrated elastin samples will further probe local anisotropic motion within elastin using a well defined NMR radiofrequency pulse sequence. NMR spin counting experiments will be used to measure the average number of nuclear spins that are clustered in the waters of hydration surrounding elastin due to the presence of a partially averaged dipolar interaction. The experimental findings of this study will be used to test and develop models of elastin, and the role of water in its function. The materials and methods necessary for this study draw upon pulse sequences well-known in the NMR community, as well as hardware that was built in the previously funded stage. The PI of the effort has a significant amount of experience in such experimental work from graduate studies, postdoctoral work and from his first two years as a junior faculty member at York College. The experiments will make use of two already existing NMR magnets and spectrometers with solid- state capabilities that are available on the college campus. PROJECT NARRATIVE This research effort seeks to investigate fundamental knowledge about the nature and behavior of elastin, a biopolymer that that confers elasticity to many vertebrate tissues. The elasticity of this biopolymer is known to be correlated to hydration, and the aim of the effort is to characterize the dynamics of water in the biopolymer. The proposal fits well within the overall goals of the National Institute of Health for fundamental research into the nature and behavior of living systems for extending healthy life and reducing the burdens of illness and disability.

描述（由申请人提供）：弹性蛋白是在各种脊椎动物组织中发现的弹性纤维的主要蛋白质。已知这种主要是疏水性蛋白质的水化水平在其结构和功能中起重要作用。各种组织的许多疾病，如心脏，已被证明与弹性蛋白的结构降解有关。拟议的研究工作的广泛目标是使用新的强脉冲梯度q空间核磁共振（NMR）成像，自旋计数NMR和氘双量子过滤NMR研究来研究水在弹性蛋白的结构和功能中的作用。Q空间成像是用于测量样品中分子位移的概率的公知方案。该技术已在先前资助的项目中成功实施，以测量弹性蛋白纤维中孔隙的平均表面积与体积比。该技术将扩展到测量的表面张力的水限制在孔的弹性蛋白通过机械应力的样品。氘水合弹性蛋白样品上的双量子滤波NMR将使用明确定义的NMR射频脉冲序列进一步探测弹性蛋白内的局部各向异性运动。NMR自旋计数实验将用于测量由于部分平均偶极相互作用的存在而聚集在弹性蛋白周围的水合沃茨中的核自旋的平均数。这项研究的实验结果将用于测试和开发弹性蛋白模型，以及水在其功能中的作用。本研究所需的材料和方法借鉴了核磁共振界众所周知的脉冲序列，以及在先前资助阶段建造的硬件。PI的努力有大量的经验，在这样的实验工作，从研究生学习，博士后工作，并从他的头两年作为一个初级教员在约克学院。实验将利用两个已经存在的核磁共振磁铁和光谱仪与固态能力，可在大学校园。本研究旨在研究弹性蛋白的性质和行为的基本知识，弹性蛋白是一种生物聚合物，赋予许多脊椎动物组织弹性。已知这种生物聚合物的弹性与水合作用相关，并且努力的目的是表征生物聚合物中水的动力学。该提案完全符合国家卫生研究所的总体目标，即对生命系统的性质和行为进行基础研究，以延长健康寿命，减少疾病和残疾的负担。