NMR studies of the effects of mode of birth on vaginal elastin

出生方式对阴道弹性蛋白影响的核磁共振研究

• 批准号: 8472680
• 负责人: Gregory Boutis
• 金额: $ 27.48万
• 依托单位: BROOKLYN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-01-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8472680
• 关键词: Affect; Alanine; Architecture; Biology; Birth; Carbon; Cattle; Characteristics; Chemicals; Chemistry; Degenerative Disorder; Dependence; Deuterium; Development; Diffusion; Disease; Elastic Fiber; Elasticity; Elastin; Elastomers; Exhibits; Functional disorder; Funding; Glycine; Goals; Health; Heart; Hereditary Disease; Human; Hydration status; Intervention; Investigation; Ligaments; Lung; Lysine; Magic; Manuscripts; Marfan Syndrome; Measurement; Measures; Menopause; Methodology; Methods; Modeling; Motion; Mus; Nuclear Magnetic Resonance; Organ; Outcome; Pelvis; Physics; Physiologic pulse; Play; Postpartum Period; Pregnancy; Proline; Property; Proteins; Ptosis; Publishing Peer Reviews; Pulmonary Emphysema; Quality of life; Relaxation; Reporting; Request for Applications; Research; Research Methodology; Residual state; Role; Secondary Protein Structure; Simulate; Skin; Solvents; Source; Spectrum Analysis; Sprague-Dawley Rats; Structure; Surface; Techniques; Technology; Time; Time Study; Tissues; United States; United States National Institutes of Health; Vagina; Vaginal delivery procedure; Valine; Vertebral column; Water; Woman; Work; base; cost; crosslink; experience; innovation; insight; nanopore; professor; public health relevance; quantum; reproductive; simulation; solid state; two-dimensional; water diffusion

DESCRIPTION (provided by applicant) The management and treatment of pelvic organ prolapse in the United States currently exceeds one billion dollars in costs annually. Elastin, the principle protein component of the elastic fiber, imparts vertebrate tissues such as the heart, lungs and skin with remarkable elasticity. The loss of elastin architecture and function is a pathological characteristic of a number of human degenerative and genetic disorders, and is believed to play a critical role in the vagina and in the pathophysiology of pelvic organ prolapse. The central hypothesis of this proposal is that vaginal birth degrades structural and dynamical properties of vaginal elastin compared to cesarean delivery. The objectives of this proposal are to quantitatively investigate the effects of modes of delivery on the structure of elastin of the vagina by pursuing two independent aims. The first aim of the proposal is to measure the structural and dynamical properties of lysine, proline, valine, glycine and alanine rich segments of elastin of the vagina following various modes of delivery. A key characteristic of this protein that distinguishes it from other elastomers is that its function hinges on hydration only hydrated elastin retains its elasticity. The second aim of the proposal is to measure the dynamics of water in vaginal elastin following various modes of delivery. This project will make use of nuclear magnetic resonance methods for these measurements. The proposal is interdisciplinary and encompasses biology, chemistry and physics. The methodology includes conventional carbon-13 nuclear magnetic resonance techniques that rely on magic angle spinning technology. In addition, the project will involve the use of non-commercial pulsed gradient fields, developed by the PI, to study time-dependent diffusion of water in elastin. The PI of the proposal is an Assistant Professor of Physics with significant expertise in nuclear magnetic resonance methodology and research experience in elastin. The PI was previously funded for four years under an NIH SC1 proposal. This proposal is a renewal application requesting four additional years of funding. This continued funding will assist the PI to transition to non-SCORE support by following a stepwise developmental plan provided in the biosketch.

描述（由申请人提供）在美国，骨盆器官脱垂的管理和治疗目前每年花费超过10亿美元。弹性蛋白,