Acquisition of a combined AFM/light microscope for research and education

购买用于研究和教育的 AFM/光学组合显微镜

• 批准号: 8247282
• 负责人: Denis Wirtz
• 金额: $ 34.22万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8247282
• 关键词: Accounting; Advisory Committees; Biological Process; Biology; Cadherins; Cell Shape; Cell division; Cell-Cell Adhesion; Cells; Communities; Core Facility; Cytoskeletal Proteins; Defect; Education; Engineering; Equilibrium; Funding; Genes; Housing; Image; Institutes; Internet; Laboratories; Light Microscope; Maintenance; Mechanics; Mediating; Medicine; Microscope; Nanotechnology; Neoplasm Metastasis; Reporting; Research; Resolution; Role; Scanning; Scanning Probe Microscopes; Schedule; Selectins; Services; Spectrum Analysis; Supervision; Surface; The Sun; United States National Institutes of Health; Universities; disease-causing mutation; instrument; nano; nanobiotechnology; outreach; single molecule; square foot

DESCRIPTION (provided by applicant): We propose to acquire an Asylum Research MFP-3D atomic force microscope (AFM), which has high- resolution force spectroscopy and scanning capabilities, integrated into a Nikon TI-E epifluorescence microscope for a combined AFM/microscope instrument. This MFP-3D/Nikon instrument can apply calibrated forces ranging between pico- and nano-newton, a force range relevant to many biological processes. Unlike our current stand-alone non-imaging MFP-1D AFM instrument, the combined MFP-3D/Nikon instrument allows for both imaging and non-imaging applications. The MFP-3D/Nikon instrument will directly support a wide range of applications that have recently been funded by the NIH. These applications include (1) Mechanistic studies of the defects in cell mechanics induced by disease-causing mutations in the Lmna gene by Dr. Wirtz's group (1R01GM084204), (2) Biophysical studies of selectin-mediated cell-cell adhesion in cancer metastasis by Dr. Konstantopoulos' group (5R01CA101135), (3) High-resolution imaging of surfaces covered with engineered bacterial cells by Dr. Ostermeier's group 5R01GM066972), (4) Biophysical studies of the mechanical role of cytoskeletal proteins in controlling bacterial cell shape and mediating cell division by Dr. Sun's group (5R01GM075305), as well as (5) Single-molecule force spectroscopy studies of cadherin- mediated cell-cell adhesion by Dr. Wirtz group (1R21CA13789). This combined instrument will be placed in an 800 sq ft state-of-the-art facility NanoBio Lab of the Institute for NanoBioTechnology (INBT) in the NEB Building at the Johns Hopkins University Homewood campus. This core facility has a demonstrated outstanding record of providing excellent imaging service to the INBT research community, offering highly popular laboratory courses in nanoscale science and engineering for applications in biology and medicine, and participating in large-scale outreach efforts. Housing the combined AFM/microscope in the INBT NanoBio Lab directed by D. Wirtz will greatly enhance its efficient and balanced use by the four primary NIH- funded users and other users under the supervision of an advisory committee. The INBT will provide support for the maintenance of the instrument, will offer the laboratory space, and will provide the administrative/accounting/web support for the management of the facility, scheduling of users, and financial reporting. PUBLIC HEALTH RELEVANCE: The requested MFP-3D/Nikon instrument will be used in a wide range of biomedical science projects of importance to human diseases, including cancer, laminopathies, and bacterial and viral infection.

描述（由申请人提供）：我们建议获得一台Asylum Research MFP-3D原子力显微镜（AFM），该显微镜具有高分辨率力谱和扫描能力，集成到Nikon TI-E落射荧光显微镜中，用于组合AFM/显微镜仪器。这台MFP-3D/尼康仪器可以施加皮科和纳牛顿之间的校准力，这是一个与许多生物过程相关的力范围。与我们目前的独立非成像MFP-1D AFM仪器不同，MFP-3D/Nikon组合仪器可用于成像和非成像应用。MFP-3D/尼康仪器将直接支持最近由NIH资助的广泛应用。这些应用包括（1）维尔茨博士的小组对Lmna基因致病突变引起的细胞力学缺陷的机理研究（1 R 01 GM 084204），（2）Konstantopoulos博士小组对癌症转移中选择素介导的细胞-细胞粘附的生物物理学研究（5 R 01 CA 101135），（3）Ostermeier博士的小组对覆盖有工程细菌细胞的表面进行高分辨率成像（5 R 01 GM 066972），（4）孙博士研究组对细胞骨架蛋白在控制细菌细胞形状和介导细胞分裂中的机械作用的生物物理研究（5 R 01 GM 075305），以及（5）维尔茨博士小组对钙粘蛋白介导的细胞-细胞粘附的单分子力谱研究（1 R21 CA 13789）。该组合仪器将被放置在约翰霍普金斯大学霍姆伍德校区NEB大楼的纳米生物技术研究所（INBT）的800平方英尺最先进的设施纳米生物实验室中。这个核心设施在为INBT研究界提供卓越的成像服务，为生物学和医学应用提供非常受欢迎的纳米科学和工程实验室课程，并参与大规模的外展工作方面表现出色。将AFM/显微镜组合在由D.维尔茨将在一个咨询委员会的监督下，大大提高四个主要的NIH资助用户和其他用户对其的有效和平衡使用。INBT将为仪器的维护提供支持，将提供实验室空间，并将为 设施管理、用户调度和财务报告。 公共卫生相关性：所要求的MFP-3D/Nikon仪器将用于对人类疾病具有重要意义的广泛生物医学科学项目，包括癌症、核纤层蛋白病以及细菌和病毒感染。