Spectral Laser Scanning Biological Microscope

光谱激光扫描生物显微镜

• 批准号: 8246290
• 负责人: Mary A Raven
• 金额: $ 42.95万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2013-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246290
• 关键词: Aging; Biological; Caenorhabditis elegans; Cell Culture Techniques; Collaborations; Dyes; Fluorescent Probes; Funding; Funding Applicant; Image; Lasers; Life; Location; Mammalian Cell; Marine Invertebrates; Microscope; Organism; Proteins; Quantum Dots; Research; Research Personnel; Resolution; Sampling; Scanning; Specimen; Speed; Staging; Support System; System; United States National Institutes of Health; instrument; meetings; programs; success

DESCRIPTION (provided by applicant): Funds are requested to purchase an Olympus Fluoview 1000S confocal instrument with state-of-the art functionality to replace an aging shared system that can no longer meet the research demands of our NIH funded researchers. There is a general demand for the increased speed and sensitivity that the Fluoview 1000S will provide. Additionally, collaborations across fields have broadened the research demands upon our facility and its shared confocal system. Our confocal system needs to support analyses of multiple organisms (e.g. marine invertebrates, C. elegans, and mammalian cell cultures), multiple probes (fluorescent proteins, quantum dots, and dyes) in multiple research paradigms. To provide this versatility, we have proposed a system with spectral imaging capabilities, a motorized X, Y stage and an environmental system that supports a range of imaging demands. This configuration will allow researchers to systematically sample large fields at high resolution, and to repeatedly sample multiple locations in fixed and live specimens. A replacement system is critical to insuring the on-going success of our NIH funded researchers and to supporting their biomedically relevant research programs. PUBLIC HEALTH RELEVANCE: A number of NIH funded research projects depend on access to a state-of-the art confocal microscope. The replacement of an aging discontinued confocal microscope with the proposed state-of-the-art system will facilitate numerous biomedical research projects relevant to public health including projects on cancer, wound healing, polycystic kidney disease, diabetes and Alzheimer's disease.

描述（由申请人提供）：需要资金购买具有最先进功能的奥林巴斯 Fluoview 1000S 共聚焦仪器，以取代老化的共享系统，该系统无法再满足 NIH 资助的研究人员的研究需求。 人们普遍要求 Fluoview 1000S 能够提供更高的速度和灵敏度。 此外，跨领域的合作扩大了对我们的设施及其共享共聚焦系统的研究需求。 我们的共聚焦系统需要支持多种研究范式中多种生物体（例如海洋无脊椎动物、线虫和哺乳动物细胞培养物）、多种探针（荧光蛋白、量子点和染料）的分析。 为了提供这种多功能性，我们提出了一种具有光谱成像功能的系统、电动 X、Y 平台和支持一系列成像需求的环境系统。这种配置将使研究人员能够以高分辨率系统地对大区域进行采样， 并在固定样本和活体样本的多个位置重复采样。替代系统对于确保 NIH 资助的研究人员持续取得成功并支持他们的生物医学相关研究项目至关重要。 公共健康相关性：美国国立卫生研究院 (NIH) 资助的许多研究项目都依赖于最先进的共聚焦显微镜。用提议的最先进的系统取代老化的已停产的共聚焦显微镜将促进许多与公共卫生相关的生物医学研究项目，包括癌症、伤口愈合、多囊肾病、糖尿病和阿尔茨海默病的项目。

项目成果

Synergistic antitumor activity of camptothecin-doxorubicin combinations and their conjugates with hyaluronic acid.

• DOI: 10.1016/j.jconrel.2015.04.031
• 发表时间: 2015-07-28
• 期刊: Journal of controlled release : official journal of the Controlled Release Society
• 作者: Camacho KM;Kumar S;Menegatti S;Vogus DR;Anselmo AC;Mitragotri S
• 通讯作者: Mitragotri S

Macrophage-mediated delivery of light activated nitric oxide prodrugs with spatial, temporal and concentration control.

• DOI: 10.1039/c8sc00015h
• 发表时间: 2018-04-21
• 期刊: Chemical science
• 影响因子: 8.4
• 作者: Evans MA;Huang PJ;Iwamoto Y;Ibsen KN;Chan EM;Hitomi Y;Ford PC;Mitragotri S
• 通讯作者: Mitragotri S

Analysis of the basal chordate Botryllus schlosseri reveals a set of genes associated with fertility.

• DOI: 10.1186/1471-2164-15-1183
• 发表时间: 2014-12-26
• 期刊: BMC genomics
• 影响因子: 4.4
• 作者: Rodriguez D;Sanders EN;Farell K;Langenbacher AD;Taketa DA;Hopper MR;Kennedy M;Gracey A;De Tomaso AW
• 通讯作者: De Tomaso AW

Microtubule-Targeting Agents Eribulin and Paclitaxel Differentially Affect Neuronal Cell Bodies in Chemotherapy-Induced Peripheral Neuropathy.

• DOI: 10.1007/s12640-017-9729-6
• 发表时间: 2017-07
• 期刊: Neurotoxicity research
• 影响因子: 3.7
• 作者: Benbow SJ;Wozniak KM;Kulesh B;Savage A;Slusher BS;Littlefield BA;Jordan MA;Wilson L;Feinstein SC
• 通讯作者: Feinstein SC

DAFODIL: A novel liposome-encapsulated synergistic combination of doxorubicin and 5FU for low dose chemotherapy.

• DOI: 10.1016/j.jconrel.2016.03.027
• 发表时间: 2016-05-10
• 期刊: Journal of controlled release : official journal of the Controlled Release Society
• 作者: Camacho KM;Menegatti S;Vogus DR;Pusuluri A;Fuchs Z;Jarvis M;Zakrewsky M;Evans MA;Chen R;Mitragotri S
• 通讯作者: Mitragotri S