An Accurate Mass TOF Mass Spectrometer - HPLC System for Marine Organic Chemistry

精确质量 TOF 质谱仪 - 用于海洋有机化学的 HPLC 系统

• 批准号: 8246778
• 负责人: William Fenical
• 金额: $ 32.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2013-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246778
• 关键词: Atmospheric Pressure; Bacteria; Biological Factors; Biomedical Research; California; Charge; Chemicals; Complex Mixtures; Computer software; Cyanobacterium; Electrospray Ionization; Engineering; Environment; Funding; High Pressure Liquid Chromatography; Institution; Marines; Molecular; Oceanography; Organic Chemistry; Phase; Research; Resolution; Sampling; Source; Structure; System; Time; United States National Institutes of Health; Universities; Walking; Water; genetic manipulation; instrumentation; ionization; marine natural product; mass spectrometer; microbial; novel; particle; pressure; user-friendly

DESCRIPTION (provided by applicant): To support critical NIH-funded biomedical research at Scripps Institution of Oceanography (SIO) / University of California, San Diego (UCSD), this proposal details need, provides justification and requests funding for a combined high resolution HPLC and high resolution time-of-flight (TOF) mass spectrometer system for rapid, efficient separation of sample components and accurate mass determination of a wide range of molecules derived from this research. The analytical instrumentation includes a high resolution HPLC that can readily accommodate less than 2 micron particle stationary phases operated at pressures up to 9000 psi (600 bar), which provide for effective and efficient several minute analyses of moderately to highly complex mixtures. The requested instrumentation also includes a high resolution (HR) TOF mass spectrometer (MS) with a highly sensitive electrospray ionization source or a combined atmospheric pressure electrospray and chemical ionization source which yields molecular formulas with sub-1 ppm accuracy of unknown and known compounds ranging in polarity from nonpolar organic molecules to highly-charged water-soluble molecules with mass-to-charge ratios of up to 20,000. In a multi-user, "walk- up" environment that is supported by user-friendly open-access software, this LC / HRMS system will be utilized extensively for the rapid determination of molecular formulas for novel marine natural products, efficient "dereplication" of known natural products from marine bacteria and cyanobacteria, identification of microbial metabolites in low concentrations following genetic manipulation, and structure determination of semisynthetic, synthetic and genetically engineered natural products. PUBLIC HEALTH RELEVANCE: This application requests NIH support for the replacement of an aging and obsolete Hewlett-Packard (Agilent) 1100 low resolution LC-MS with an updated Agilent 6230AA LC-MS to be used by NIH sponsored marine biomedical researchers. The proposed instrument, which will be located in the CMBB core, will provide molecular formula data and dramatically shorten analysis times, and accelerate the research of more than 40 faculty, staff, students and postdocs within the Center for Marine Biotechnology and Biomedicine (CMBB) at SIO.

描述（申请人提供）：为了支持Scripps海洋学研究所（SIO）/加州大学圣地亚哥分校（UCSD）的NIH资助的关键生物医学研究，该提案详细说明了需要，提供了理由并要求为组合的高分辨率HPLC和高分辨率飞行时间（TOF）质谱仪系统提供资金，有效分离样品组分并准确测定本研究中衍生的各种分子的质量。分析仪器包括一个高分辨率的HPLC，可以很容易地容纳小于2微米的颗粒固定相，在高达9000 psi（600 bar）的压力下运行，这提供了有效和高效的几分钟分析中度到高度复杂的混合物。所要求的仪器还包括具有高灵敏度电喷雾电离源或组合的大气压电喷雾和化学电离源的高分辨率（HR）TOF质谱仪（MS），其产生极性范围从非极性有机分子到质荷比高达20的高电荷水溶性分子的未知和已知化合物的低于1 ppm精度的分子式，000.在一个由用户友好的开放式软件支持的多用户“步行”环境中，该LC / HRMS系统将广泛用于快速确定新的海洋天然产物的分子式，从海洋细菌和蓝细菌中有效地“去复制”已知的天然产物，在遗传操作后鉴定低浓度的微生物代谢物，半合成、合成和基因工程天然产物的结构测定。 公共卫生关系：本申请请求NIH支持将老化和过时的Hewlett-Packard（Agilent）1100低分辨率LC-MS更换为更新的Agilent 6230 AA LC-MS，以供NIH赞助的海洋生物医学研究人员使用。拟议的仪器将位于CMBB核心，将提供分子式数据，大大缩短分析时间，并加速SIO海洋生物技术和生物医学中心（CMBB）内40多名教职员工，学生和博士后的研究。