An Accurate Mass TOF Mass Spectrometer - HPLC System for Marine Organic Chemistry

精确质量 TOF 质谱仪 - 用于海洋有机化学的 HPLC 系统

• 批准号: 8246778
• 负责人: William Fenical
• 金额: $ 32.45万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-05-15 至 2013-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8246778
• 关键词: Atmospheric Pressure; Bacteria; Biological Factors; Biomedical Research; California; Charge; Chemicals; Complex Mixtures; Computer software; Cyanobacterium; Electrospray Ionization; Engineering; Environment; Funding; High Pressure Liquid Chromatography; Institution; Marines; Molecular; Oceanography; Organic Chemistry; Phase; Research; Resolution; Sampling; Source; Structure; System; Time; United States National Institutes of Health; Universities; Walking; Water; genetic manipulation; instrumentation; ionization; marine natural product; mass spectrometer; microbial; novel; particle; pressure; user-friendly

DESCRIPTION (provided by applicant): To support critical NIH-funded biomedical research at Scripps Institution of Oceanography (SIO) / University of California, San Diego (UCSD), this proposal details need, provides justification and requests funding for a combined high resolution HPLC and high resolution time-of-flight (TOF) mass spectrometer system for rapid, efficient separation of sample components and accurate mass determination of a wide range of molecules derived from this research. The analytical instrumentation includes a high resolution HPLC that can readily accommodate less than 2 micron particle stationary phases operated at pressures up to 9000 psi (600 bar), which provide for effective and efficient several minute analyses of moderately to highly complex mixtures. The requested instrumentation also includes a high resolution (HR) TOF mass spectrometer (MS) with a highly sensitive electrospray ionization source or a combined atmospheric pressure electrospray and chemical ionization source which yields molecular formulas with sub-1 ppm accuracy of unknown and known compounds ranging in polarity from nonpolar organic molecules to highly-charged water-soluble molecules with mass-to-charge ratios of up to 20,000. In a multi-user, "walk- up" environment that is supported by user-friendly open-access software, this LC / HRMS system will be utilized extensively for the rapid determination of molecular formulas for novel marine natural products, efficient "dereplication" of known natural products from marine bacteria and cyanobacteria, identification of microbial metabolites in low concentrations following genetic manipulation, and structure determination of semisynthetic, synthetic and genetically engineered natural products. PUBLIC HEALTH RELEVANCE: This application requests NIH support for the replacement of an aging and obsolete Hewlett-Packard (Agilent) 1100 low resolution LC-MS with an updated Agilent 6230AA LC-MS to be used by NIH sponsored marine biomedical researchers. The proposed instrument, which will be located in the CMBB core, will provide molecular formula data and dramatically shorten analysis times, and accelerate the research of more than 40 faculty, staff, students and postdocs within the Center for Marine Biotechnology and Biomedicine (CMBB) at SIO.

描述（由申请人提供）：为了支持美国国立卫生研究院资助的Scripps海洋研究所(SIO) /加州大学圣地亚哥分校（UCSD）的重要生物医学研究，本提案详细说明了高分辨率HPLC和高分辨率飞行时间（TOF）质谱联用系统的需求，提供了理由并申请了资金，该系统可以快速、有效地分离样品成分，并对本研究中衍生的广泛分子进行准确的质量测定。分析仪器包括一个高分辨率的高效液相色谱，可以很容易地容纳小于2微米的颗粒固定相，在高达9000 psi （600 bar）的压力下工作，提供有效和高效的几分钟分析中等到高度复杂的混合物。所要求的仪器还包括高分辨率（HR） TOF质谱仪（MS），具有高灵敏度的电喷雾电离源或组合大气压电喷雾和化学电离源，可产生精度低于1ppm的未知和已知化合物的分子式，其极性范围从非极性有机分子到高电荷水溶性分子，质量电荷比高达20,000。在一个由用户友好的开放获取软件支持的多用户“漫步”环境中，该LC / HRMS系统将广泛用于快速测定新型海洋天然产物的分子式，从海洋细菌和蓝藻中高效地“去复制”已知天然产物，在遗传操作后鉴定低浓度的微生物代谢物，以及半合成的，合成和基因工程的天然产品。