Improving HbF induction by inhibiting epigenetic target enzymes

通过抑制表观遗传靶酶改善 HbF 诱导

• 批准号: 8467828
• 负责人: JOSEPH DESIMONE
• 金额: $ 145.21万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-15 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8467828
• 关键词: Address; Adult; Animal Model; Biological Factors; Cells; Chemistry; Chromatin; Clinical; Clinical Trials; Decitabine; Disease; Dose; Drug Combinations; Drug Delivery Systems; Drug Formulations; Drug usage; EPOR gene; Enzymes; Epigenetic Process; Erythroid; Erythroid Progenitor Cells; Evaluation; Event; Excision; FDA approved; Globin; Gold; Human; In Situ; In Vitro; Individual; Lead; Methods; Modeling; Modification; Mus; Organ; Papio; Peptides; Pharmaceutical Chemistry; Pharmaceutical Preparations; Repression; Safety; Schedule; Sickle Cell Anemia; Small Interfering RNA; Staging; Sulfhydryl Compounds; Therapeutic Index; Toxic effect; Toxicology; Translations; Tranylcypromine; abstracting; combinatorial; dosage; drug candidate; drug efficacy; fetal; improved; in vivo; inhibitor/antagonist; innovation; novel; pre-clinical; prevent

DESCRIPTION (provided by applicant): Pharmacologic activation of HbF can interrupt the most proximal events in the pathophysiological cascade of sickle cell disease (SCD), and is therefore a preferred approach to multi-organ disease modification. Its clinical potential, however, has not been fulfilled. We hypothesize that removal of epigenetic activation marks at the HBG locus is an early and necessary step in the fetal to adult globin "switch", and hence, inhibiting specific chromatin-modifying enzymes that remove these activating epigenetic marks (e.g. LSD1 or DNMT1) will efficiently prevent HBG repression. In SA1, we determine if there is an obligatory sequence in the placement of epigenetic marks at HBG during the maturational globin switch. Precisely staged erythroid progenitor cells are analyzed by ChIP. The interdependence of epigenetic marks is analyzed by use of drugs and siRNA knockdowns targeting the enzymes responsible for catalyzing these modifications. In SA2 we will explore the possibility that either of two recently discovered non-cytotoxic inhibitors of LSD1 could lead to more efficacious human SCD medications. One of these compounds, tranylcypromine (TC), is already FDA approved. The other compound, a natural product belonging to the chromen-4-one class and potent non-cytotoxic LSD1 inhibitor, will be improved with further SAR and medicinal chemistry prior to evaluation both in vitro and in murine models of SCD, to generate critical pre-clinical in vivo proof of principle. Thus we already have three candidate epigenetic drugs (the two LSD1 inhibitors and the DNMT1-depleting drug, decitabine) to evaluate and ask whether some combination of these compounds can synergistically induce HbF, enabling individual medication dosages to be kept at levels well clear of possible toxicity. Ideally, candidate drugs would be targeted to the erythroid compartment, to further enhance therapeutic index. This need is addressed in SA3, using a highly innovative, versatile method that connects any developed drug to an EPOR targeting peptide by way of gold nanocluster intermediaries and chemistry that does not impede the function of either, and which facilitates in situ release of drug by thiol biomolecules at target cells. In SA4 we will explore in the baboon model individual and combinatorial drug efficacy and safety, providing dose and schedule guidance for formal toxicology and formulation studies and definitive progress to clinical trials. While many pre-clinical animal models inadequately predict clinical utility, the baboon model has reproducibly facilitated clinical translation of novel therapies for SCD. By using highly innovative methods and rational concepts to develop novel drug combinations and delivery systems that can maximize therapeutic index, this project strives to fulfill the potential of HbF activation therapy. (End of Abstract)

描述（由申请人提供）： HbF的药理学活化可以中断镰状细胞病（SCD）的病理生理级联中的最近端事件，因此是多器官疾病改变的优选方法。然而，其临床潜力尚未得到充分发挥。我们假设，在HBG基因座的表观遗传激活标记的去除是在胎儿到成人珠蛋白“开关”的早期和必要的步骤，因此，抑制特定的染色质修饰酶，去除这些激活表观遗传标记（如LSD 1或DNMT 1）将有效地防止HBG抑制。在SA 1，我们确定是否有一个强制性的序列在HBG的表观遗传标记的位置在成熟的珠蛋白开关。通过ChIP分析精确分期的红系祖细胞。通过使用药物和靶向负责催化这些修饰的酶的siRNA敲低来分析表观遗传标记的相互依赖性。在SA 2中，我们将探索最近发现的两种LSD 1非细胞毒性抑制剂中的任何一种可能导致更有效的人类SCD药物。其中一种化合物，反苯环丙胺（TC），已经被FDA批准。另一种化合物是属于色烯-4-酮类的天然产物和强效非细胞毒性LSD 1抑制剂，在体外和SCD鼠模型中评价之前，将通过进一步的SAR和药物化学进行改进，以产生关键的临床前体内原理证明。因此，我们已经有三种候选表观遗传药物（两种LSD 1抑制剂和DNMT 1消耗药物地西他滨）来评估和询问这些化合物的某种组合是否可以协同诱导HbF，使个体药物剂量保持在远离可能毒性的水平。理想情况下，候选药物将靶向红细胞区室，以进一步提高治疗指数。在SA 3中解决了这一需求，使用了一种高度创新的通用方法，该方法通过金纳米团簇中间体和化学物质将任何开发的药物连接到EPOR靶向肽，该方法不会阻碍任何一种药物的功能，并且有助于通过硫醇生物分子在靶细胞处原位释放药物。在SA 4中，我们将在狒狒模型中探索个体和组合药物的疗效和安全性，为正式的毒理学和制剂研究以及临床试验的确定性进展提供剂量和时间表指导。虽然许多临床前动物模型不能充分预测临床效用，但狒狒模型可重复地促进SCD新疗法的临床转化。通过使用高度创新的方法和 合理的概念，开发新的药物组合和输送系统，可以最大限度地提高治疗指数，该项目致力于实现HbF激活治疗的潜力。(End的 摘要）