Data Management and Resource Dissemination Core

该核心的目标是提供一个中央系统，用于管理有关M中基因功能的信息。结核病（Mtb）并将其呈现给公众。预计整个计划项目将产生大量与结核分枝杆菌基因组中基因功能鉴定相关的数据。具体而言，每个组成项目将产生的主要类型的基因组规模数据包括：来自敲低突变体中转座子插入文库测序的数据;用于遗传相互作用作图的其他基因或基因组区域的重要性变化的统计估计，表型和抑制突变体的全基因组测序，以及细菌突变体的基因表达分析（RNA-Seq）。将建立一个网站，提供所产生的原始数据的访问，以及衍生信息的直观显示（热图、基因组浏览器、转座子插入密度图等）。此外，我们将专注于提供数据的统计分析，例如严格识别基因表达和基因必要性的统计学显著变化。所有这些数据将结合起来，使基因功能的推断，我们将使用基因本体论（GO）的条款。该网站将提供支持每个推论的所有证据来源的链接，以及通过GO术语分组/计数/搜索基因的工具。

The goal of this Core is to provide a central system for managing information generated about functions of genes in M. tuberculosis (Mtb) and presenting it to the public. The overall Program Project is anticipated to generate a large amount of data related to identification of functions for genes in the Mtb genome. In particular, major types of genome-scale data to be generated by each of the component projects include: data from sequencing transposon-insertion libraries in knock-down mutants; statistical estimates of changes in essentiality of other genes or regions of the genome for genetic interaction mapping, whole-genome sequencing of phenotypic and suppressor mutants, and gene expression analysis (RNA-Seq) in bacterial mutants. A web site will be developed to provide access to the raw data generated, as well as visual display of derived information (heat maps, genome browser, plots of transposon insertion density, etc.). In addition, we will focus on providing statistical analysis of the data, such as rigorous identification of statistically significant changes in gene expression and gene essentiality. All this data will be combined to make inferences of gene function, for which we will use Gene Ontology (GO) terms. The website will provides links to all sources of evidence supporting each inference, as well as tools for grouping/counting/searching genes by GO term.