The roles of biologically relevant ncRNAs

项目总结（见说明）： 本项目的目的是确定分枝杆菌中重要的非编码RNA的功能 结核最近已经清楚，非编码RNA在许多细胞中起着重要的调节作用。 原核生物然而，在结核分枝杆菌中，像大多数细菌一样，绝大多数都完全保留了 没有特征的我们已经在实验中鉴定了超过1000种Mtb中的非编码RNA，其中只有5种具有 在任何程度上进行实验表征。在本提案中，我们将重点关注一类非编码 RNA，反式作用的基因间调节RNA，通常称为小RNA（sRNA）。虽然一些 sRNA与蛋白质相互作用并影响蛋白质的功能，迄今为止表征的绝大多数sRNA 影响mRNA的稳定性和/或翻译。因此，定义生物学功能的关键步骤 regRNA首先要识别它所调节的mRNA以及这种调节的生物学后果 在转录组和蛋白质组（调节子）的水平上，然后在细胞的生理学方面。我们 我提出了一系列分层的目标，在这些目标中，我们将定义候选sRNAs的功能， 特异性首先定义标准下Mtb生长的候选者的重要性，

PROJECT SUMMARY (See instructions): The goal of this project is to define the function of biologically important noncoding RNAs in Mycobacterium tuberculosis. It has recently become clear that noncoding RNAs play important regulatory roles in many prokaryotes. However, in Mtb, like most bacteria, the vast majority of them remain completely uncharacterized. We have experimentally identified over 1000 noncoding RNAs in Mtb, of which only 5 have been experimentally characterized to any extent. In this proposal, we will focus on one class of noncoding RNAs, trans-acting, intergenic regulatory RNAs, commonly termed small RNAs (sRNAs). While some sRNAs interact with and affect the function of proteins, the vast majority of sRNAs characterized to date influence the stability and/or translation of mRNAs. Thus, the critical steps in defining the biological function a regRNA are to identify the mRNA(s) it regulates and the biological consequences of this regulation¿first at the level of the transcriptome and proteome (regulon) and then in terms of the cell's physiology. We propose a tiered series of aims in which we will define the function of candidate sRNAs with increasing specificity¿first defining the importance of the candidates for Mtb growth under standard and