Technology Core: Proteomics

我们将使用最先进的、基于定量质谱的蛋白质组学来定义野生型和突变型结核分枝杆菌菌株在各种生长和扰动条件下的蛋白质复合物的成员关系。系统和公正地定义关键蛋白复合物的成员，以及在各种生长和异常条件下该成员的变化，将为了解对结核分枝杆菌生长和存活重要的基因的生物学功能提供重要的见解。通过整合蛋白质组学、代谢组学和转录组学分析的结果，一个对生物体生长和生存至关重要的相互作用和表达程序的全面图景应该出现。

We will use state-of-the-art, quantitative mass spectrometry-based proteomics to define membership of protein complexes in wild-type and mutant Mtb strains under a variety of growth and perturbational conditions. Systematic and unbiased definifion of the membership of key protein complexes, and the changes in that membership under a variety of growth and perturbaional condtions, will provide critical insights into the biological function of genes important for growth and survival of Mtb. By integrating results of proteomics, metablomics and transcriptomic analyses, a comprehensive picture of the interactions and expression programs critical for growth and survival of the organism should emerge. We will also use state-of-the-art, quantitative global proteomics to profile auxotrophic strains of Mtb to understand the roles of genes encoding proteins and non-coding RNAs of unknown function in M. tuberculosis. We will choose condtions/time points just before changes in growth occur to minimize the possibility that changes in protein abundance are a consequence of changes in growth and survival but, instead, are directly attributable to the mutation. Both wild type and mutant bacteria strains will be analyzed by comparative quantitative proteomic analysis. We anticipate that we will find several different types of changes. We will correlate changes with observations from other experiments across the consortium.