Biospecimen (Visscher)

生物样本 (Visscher)

• 批准号: 8744971
• 负责人: JAMES Newell INGLE
• 金额: $ 25.16万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-22 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8744971
• 关键词: Aliquot; Animal Model; Antibodies; Arts; Benign; Biological Assay; Biological Markers; Biometry; Biopsy; Biostatistics Core; Blood specimen; Breast; Cancer Control; Cancer Patient; Cataloging; Catalogs; Cell Line; Cells; Clinic; Collaborations; Collection; Consent; DNA; Data; Data Element; Databases; Development; ERBB2 gene; Electronics; Ensure; Equipment; Evaluation; Formalin; Freezing; Frozen Sections; Funding; Genomics; Grant; Histology; Human Cell Line; Immunohistochemistry; Laboratories; Malignant - descriptor; Mammary Gland Parenchyma; Mayo Clinic Cancer Center; Measures; Molecular Analysis; Operative Surgical Procedures; Paraffin; Paraffin Embedding; Pathologic; Pathologist; Pathology; Patients; Process; Proteins; Protocols documentation; Qualifying; Quality Control; RNA; Research Infrastructure; Research Personnel; Research Project Grants; Resource Sharing; Resources; Sampling; Serum; Services; Specimen; Staging; Staining method; Stains; Testing; Tissue Microarray; Tissue Sample; Tissues; Translational Research; Work; Xenograft procedure; abstracting; base; career development; cohort; digital imaging; experience; malignant breast neoplasm; operation; patient registry; programs; repository; research and development; tumor xenograft; validation studies

The Biospecimen and Pathology Core ofthe Breast SPORE is responsible for accessioning and processing new biospecimens for the Breast SPORE Biospecimen Repository and to make those specimens available for use in the four SPORE main projects and very likely for the Developmental Research and Career Development Programs. Biospecimens will also be made available to other intra- and extra-mural investigators engaged in breast cancer translational research. Requests for biospecimens will be taken under consideration by the Breast SPORE Operations Committee, with a heavy emphasis on scientific merit and on availability of specimens and their associated data needed for analysis. Input from the Biostatistics and Patient Registry Core is included in the evaluation of requests for tissues. The Pathology Team of the Biospecimen Core will provide detailed annotations in the Breast Pathology Database for frozen and formalin-fixed paraffin-embedded tissues from selected cohorts of patients to support SPORE projects and additional projects for which the Breast Biospecimen Repository has provided tissues. The Core pathology team will also interpret IHC staining. The Biospecimen and Pathology Core will coordinate with the Mayo Clinic Cancer Center Biospecimen Accessioning and Processing Shared Resource to process blood samples to provide genomic DNA and serum aliquots, and with the Tissue and Cell Molecular Analysis Shared Resource to provide histology and other tissue-based services, including paraffin and frozen sectioning, immunohistochemistry, tissue microarray construction, and digital imaging. Working closely with existing infrastructure such as these shared resources minimizes redundancy of services and utilizes existing experience and state ofthe art equipment. New immunostaining assays will be developed by the Biospecimen and Pathology Core, as well as construction of cell line tissue microarrays to screen and test antibodies for immunostaining formalin-fixed, paraffin-embedded tissues.

Breast SPORE的生物标本和病理学核心负责为Breast SPORE生物标本库登记和处理新的生物标本，并使这些标本可用于四个SPORE主要项目，很可能用于发育研究和职业发展计划。生物标本也将提供给其他内部和外部的研究人员从事乳腺癌转化研究。乳腺孢子运营委员会将考虑生物标本的申请，重点是科学价值和标本的可用性及其分析所需的相关数据。来自生物统计学和患者登记中心的输入包括在组织申请的评价中。生物样本核心的病理学团队将在乳腺病理学数据库中为选定患者队列的冷冻和福尔马林固定石蜡包埋组织提供详细注释，以支持SPORE项目和乳腺生物样本库提供组织的其他项目。核心病理学团队还将解读IHC染色。生物标本和病理学核心将与马约诊所癌症中心生物标本采集和处理共享资源协调，以处理血液样本，提供基因组DNA和血清等分试样，并与组织和细胞分子分析共享资源协调，以提供组织学和其他基于组织的服务，包括石蜡和冷冻切片、免疫组织化学、组织微阵列构建和数字成像。与现有的基础设施（如这些共享资源）密切合作，最大限度地减少服务冗余，并利用现有的经验和最先进的设备。生物标本和病理学核心将开发新的免疫染色测定法，以及细胞系组织微阵列的构建，以筛选和测试福尔马林固定的石蜡包埋组织的免疫染色抗体。