Histology - lmmunohistochemistry Module

组织学-免疫组织化学模块

• 批准号: 8539815
• 负责人: Kar-Ming Fung
• 金额: $ 20.77万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8539815
• 关键词: Animal Model; Antibodies; Area; Biochemical; Cancer Biology; Cell model; Cultured Cells; Evaluation; Freezing; Funding; Histology; Life; Malignant Neoplasms; Mentors; Microscope; Microscopic; Microscopy; Modeling; Oklahoma; Paraffin Embedding; Phase; Research Personnel; Slide; Staining method; Stains; Tissues; Training; animal tissue; anticancer research; cellular imaging; instrument; tissue culture; tissue processing

The purpose of the Histology, Immunostaining, Microscopy Core is to provide to the Promising Junior Investigators (PJIs) and cancer funded investigators, tissue processing, embedding, sectioning, histochemical staining of mounted slides, developing immunohistochemical (IHC) (brighfield or fluorescent) staining for paraffin embedded and frozen tissues and immunocytochemical (ICC) (brighfield or fluorescent) staining for cultured cells, evaluation of new antibodies for IHC staining, biochemical and special staining. The core will provide technical support to all scientific investigators in the above said areas; with PJIs having first priority. The core will also provide training on and use of various microscopes including conventional bright field, phase and epifluorescence microscopy as well as new instruments for live cell imaging. This facility is critical for cancer investigators using animal models and tissue culture models to study basic mechanisms of cancer biology.

组织学、免疫染色、显微镜核心的目的是为有前途的初级研究者（PJIs）和癌症资助的研究者提供组织处理、包埋、切片、固定载玻片的组织化学染色、免疫组织化学（IHC）显影石蜡包埋和冷冻组织的（亮场或荧光）染色和免疫细胞化学（ICC）用于培养细胞的荧光染色（brighfield或荧光）、用于IHC染色的新抗体的评估、生物化学和特殊染色。该核心将为上述领域的所有科学调查人员提供技术支持; PJIs具有优先权。该中心还将提供各种显微镜的培训和使用，包括传统的明场、相位和落射荧光显微镜以及用于活细胞成像的新仪器。该设施对于癌症研究人员使用动物模型和组织培养模型研究癌症生物学的基本机制至关重要。