Histology and Immunohistochemistry Core

组织学和免疫组织化学核心

• 批准号: 10455518
• 负责人: Kar-Ming Fung
• 金额: $ 26.3万
• 依托单位: UNIVERSITY OF OKLAHOMA HLTH SCIENCES CTR
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-09-05 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10455518
• 关键词: Algorithms; Animal Model; Antibodies; Biochemical; Biological; Cancer Biology; Centers of Research Excellence; Computer Assisted; Computer software; Consultations; Cultured Cells; Data; Decalcification; Disease; Enzymes; Evaluation; Experimental Designs; Faculty; Formalin; Freezing; Genes; Head; Histocytochemistry; Histologic; Histological Techniques; Histology; Human; Image Analysis; Immunochemistry; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Individual; Infiltration; Investigation; Investigational Therapies; Laboratories; Malignant - descriptor; Malignant Neoplasms; Manuals; Mentors; Methodology; Methods; Microscope; Microscopy; Mission; Modeling; Modernization; Neoplasm Metastasis; Oklahoma; Optics; Paraffin; Paraffin Embedding; Pathologic; Pathologist; Pathology; Phase; Phenotype; Procedures; Prognostic Marker; Quantitative Evaluations; RNA; Research; Research Personnel; Resource Sharing; Sampling; Scanning; Services; Slide; Specimen; Stains; Techniques; Technology; Tissue Fixation; Tissue Microarray; Tissue Sample; Tissues; Training; Tumor Tissue; animal tissue; anticancer research; base; cancer cell; diagnostic biomarker; digital; experience; histological stains; in vivo monitoring; instrument; interest; live cell imaging; member; neoplastic cell; novel diagnostics; programs; response; skills; tissue culture; tissue processing; translational cancer research; tumor; tumor microenvironment; whole slide imaging

ABSTRACT The Histology, Immunochemistry, and Microscopy core (HIM Core) of the COBRE provides the COBRE investigators and other cancer researchers in Oklahoma with high quality histology, immunochemistry, and microscopy services. This core is directed by Dr. Fung, who is a board-certified pathologist who has more than 28-years of experience in research with histologic techniques. The HIM Core has a well-equipped laboratory that provides histologic sectioning, conventional staining, immunohistochemistry, immunofluorescence, in situ hybridization, combined immunohistochemistry and in situ hybridization, enzyme histochemistry, and other contemporary staining techniques. Most of these staining procedures are automated in order to maintain a high quality. This core also provides whole slide scanning service supported by image analysis with a choice of multiple algorithms and tissue microarray segmentation softwares. The director of the core also provides technical advice on experimental design and methodologies appropriate for the project. The core also provides training on the use of various microscopes including conventional bright-field, phase- and epifluorescence- microscopy as well as new instruments for live cell imaging. This is the only such facility available in the state of Oklahoma. Established in 2012, under the auspices of Phase I of this COBRE, the core has proven to be critical to meet the scientific needs of the COBRE investigators in addition to catering the needs of other cancer researchers in Oklahoma. During Phase I of this COBRE, 6 COBRE Promising-Junior or target investigators and 64 non-COBRE investigators used this core. The mission of the HIM Core is accomplished under the following three specific aims: 1. Consultation on experimental design and interpretation of results; 2. Histological methods and staining services; and 3. Whole slide scanning, image analysis, and live cell imaging services.

摘要 眼镜蛇的组织学、免疫化学和显微镜核心(HIM核)提供了眼镜蛇 俄克拉荷马州的研究人员和其他癌症研究人员提供高质量的组织学、免疫化学和 显微镜服务。这个核心是由冯医生指导的，他是一名获得董事会认证的病理学家，他有超过 28年组织学技术研究经验。HIM核心拥有设备齐全的实验室 提供组织切片、常规染色、免疫组织化学、免疫荧光、原位 杂交、免疫组织化学和原位杂交、酶组织化学等 现代染色技术。大多数染色程序都是自动化的，以保持较高的 质量。该核心还提供由图像分析支持的完整幻灯片扫描服务，可选择 多种算法和组织微阵列分割软件。核心主任还提供了 就适合该项目的实验设计和方法提供技术建议。核心还提供 培训各种显微镜的使用，包括常规的亮场显微镜、位相显微镜和荧光显微镜- 显微镜以及活细胞成像的新仪器。这是该州唯一可用的此类设施 俄克拉何马州。成立于2012年，在科布雷第一阶段的主持下，核心已被证明是 对于满足科布雷调查人员的科学需求以及满足其他人的需求至关重要 俄克拉荷马州的癌症研究人员。在此Cobre的第一阶段，6 Cobre前景看好-初级或目标 侦查员和非科伯尔侦查员使用了这一核心。HIM核心的使命完成了 在以下三个具体目标下：1.咨询实验设计和结果解释； 组织学方法和染色服务；以及3.全片扫描、图像分析和活细胞成像 服务。