Traditional Service Core [Translational Service Core (TSC)]

为了确保整个研究计划的成功，以及从替补席到床边的快速翻译进展，我们在当前的申请中建议建立翻译服务核心(TSC)。TSC的主要目标是标准化甲基苯丙胺滥用研究中心(MARC)的程序，以收集人类神经精神数据，处理和存储人类血液，并测量人类和啮齿动物样本中的免疫因素和反应。第二个目标是建立一个人类神经精神病学数据和血液样本的储存库。这些与神经精神病学数据相关的未确认样本将可用于未来的研究，因此对MARC具有重要的长期价值。所有三个拟议的科学组件(组件7、8和9)都分析人类或老鼠组织，以评估免疫因素和神经精神结果(例如，认知、焦虑)之间的关系。三个拟议的科学组成部分中的两个(组成部分7和8)分析来自人类受试者的神经精神病学数据。这些构成部分和MARC将受益于一个集中的技术支助委员会，以消除多余的征聘工作，使业务程序标准化，并改进跨构成部分和翻译分析。TSC将300名受试者分为三组(100人/组)：1)积极使用甲基苯丙胺(MA)的成年人，2)MA依赖早期缓解的成年人，3)不依赖的成年人。TSC的工作人员将对受试者进行神经精神病学研究访问，包括：1)临床访谈以收集生物心理社会史，2)结构化临床访谈以验证精神和药物使用障碍，3)精神症状问卷，4)涵盖所有认知领域的神经心理测试，5)医学实验室测试，以及6)采集血浆和外周血单核细胞的抽血。样本将储存在TSC储存库中，并转移到服务于其目标的组件和试点项目。TSC工作人员还将指导使用标准化方法(例如，ELISA、Luminex、Elispot和FACS)对人类和啮齿动物组织进行免疫因子表达的分析，从而提高效率和组件之间的协作。

To ensure the success of the overall research program, and rapid translational progress from the bench to the bedside, we propose in the current application the establishment of a Translational Service Core (TSC). The primary objective of the TSC is to standardize the Methamphetamine Abuse Research Center's (MARC's) procedures for collecting human neuropsychiatric data, processing and storing human blood, and measuring immune factors and responses in human and rodent samples. A secondary objective is to establish a repository for human neuropsychiatric data and blood samples. These de-identified samples, linked to the neuropsychiatric data, will be available for use in future studies, and therefore have significant long-term value to the MARC. All three proposed Scientific Components (Components 7, 8, & 9) analyze human or mouse tissue to evaluate the relationship between immune factors and neuropsychiatric outcomes (e.g., cognition, anxiety). Two of the three proposed Scientific Components (Components 7 & 8) analyze neuropsychiatric data from human subjects. These Components and the MARC would benefit from a centralized TSC to eliminate redundant recruitment efforts, standardize operating procedures, and improve cross-component and translational analyses. The TSC will recuit 300 subjects into three groups (100/group): 1) adults actively using methamphetamine (MA), 2) adults in early remission from MA dependence, and 3) non-dependent adults. TSC staff will run subjects through a neuropsychiatric study visit including: 1) a clinical interview to collect biopsychosocial history, 2) a structured clinical interview to verify psychiatric and substance use disorders, 3) psychiatric symptom questionnaires, 4) neuropsychological tests covering a full range of cognitive domains, 5) medical laboratory tests, and 6) a blood draw for collection of plasma and peripheral blood mononuclear cells. Samples will be stored in the TSC repository and transferred to Components and Pilot Projects in service of their objectives. The TSC staff will also guide analyses of human and rodent tissue for immune factor expression using standardized methods (e.g., ELISA, Luminex, ELISPOT, and FACS), thus increasing efficiency and collaboration among Components.