BIOMEDICAL ANALYSIS OF HUMAN BIOSPECIMENS FOR DIPHR

利用该合同的任务是分析先兆子痫的钙样本 预防试验 (CPEP) 测量总 25(0H)D、其亚型（25(0H)D2 和 25(0H)D3）， 多达 992 份血清样本中的维生素 D 结合蛋白 (VDBP)、白蛋白和总钙 CPEP 试验。该任务的执行期预计为6个月。 尤尼斯·肯尼迪·施赖弗国立大学校内人口健康研究部 儿童健康与人类发展研究所包含三个分支机构：1) 流行病学分会； 2）生物统计学和生物信息学分支和3）预防研究 分支机构[http://www.nichd.nih.gov/about/org/despr/]。该部门的核心任务是 设计、实施和指导流行病学、行为和生物统计研究 专注于生殖、围产期和儿科流行病学研究以及研究 解决儿童和青少年的健康行为。 该司此前曾进行过钙预防子痫前期 (CPEP) 试验 1992-1995 年，美国 5 家诊所测试了钙补充剂对风险和风险的影响。 先兆子痫（PE）的严重程度。 CPEP 研究地点之一（田纳西州孟菲斯）进行了一项 “胎儿骨矿化”的辅助研究，母亲和新生儿都可以 接受双能 X 射线吸收测定法 (DXA) 扫描。多项研究表明。 怀孕期间缺乏维生素 D 的女性往往会生出出生体重较低的婴儿， 尽管证据仍然存在争议。为了以之前的发现为基础，我们将测量 母体 CPEP 血清样本中的维生素 D 生物标志物，用于调查母体维生素 D 状态 与新生儿体脂肪和新生儿骨矿物质含量有关。母体维生素 D 还将检查与母体骨矿物质含量相关的状况。血清样本 本试验中纵向收集的数据将提供整个过程中维生素 D 状态的多种衡量标准 妊娠期使我们能够更好地了解这些女性的维生素 D 特征。这些 措施还将用于描述维生素 D 生物标志物的轨迹 妊娠分层按两个种族群体即白人和非裔美国人进行。血清样本 试验结果已储存在 NICHD 生物储存库中，温度为 -70°C。的等分试样 参与者将被运送到实验室

The tasks to utilize this contract are to analyze samples from the Calcium for Preeclampsia Prevention trial (CPEP) to measure total 25(0H)D, its isoforms (25(0H)D2 and 25(0H)D3), Vitamin D binding protein (VDBP), albumin, and total calcium in up to 992 serum samples from the CPEP trial. Period of performance for this task is estimated to be 6 months. The Division of lntramural Population Health Research of the Eunice Kennedy Shriver National Institute of Child Health and Human Development encompasses three branches: 1) Epidemiology Branch; 2) Biostatistics and Bioinformatics Branch and 3) Prevention Research Branch [http://www.nichd.nih.gov/about/org/despr/]. Central to the Division's mission is to design, implement, and direct epidemiologic, behavioral and biostatistical research which focuses on reproductive, perinatal and pediatric epidemiologic research as well as research addressing child and adolescent health behaviors. The Division previously conducted the Calcium for Preeclampsia Prevention (CPEP) trial from 1992-1995 across 5 clinics in the US testing the effect of calcium supplementation on risk and severity of preeclampsia (PE). One of the CPEP study sites (Memphis, TN) conducted an ancillary study of' Fetal bone mineralization' whereby both the mother and her newborn could receive a dual-energy X-ray absorptiometry (DXA) scan. Several studies have shown that . women with vitamin D deficiency during pregnancy tend to have infants with lower birth weight, although the evidence is still controversial. To build on previous findings, we will measure maternal CPEP serum samples for vitamin D biomarkers to investigate maternal vitamin D status in association with neonatal body fat and neonatal bone mineral content. Maternal vitamin D status will also be examined in association with maternal bone mineral content. Serum samples collected longitudinally in this trial will offer several measures of vitamin D status throughout gestation enabling us to better characterize the vitamin D profile of these women. These measures will also be used to characterize the trajectories of vitamin D biomarkers across gestation stratifying by two racial groups namely Whites and African-Americans. Serum samples from the trial have been stored in the NICHD Bio-repository at -70¿C. Aliquots of the participants will be shipped to the laboratory