BIOMEDICAL ANALYSIS OF HUMAN BIOSPECIMENS FOR DIPHR

利用这份合同的任务是分析先兆子痫患者的钙样本 预防试验(CPEP)测量总的25(0H)D及其亚型(25(0H)D2和25(0H)D3)， 来自中国的992份血清样本中的维生素D结合蛋白(VDBP)、白蛋白和总钙 CPEP审判。这项任务的执行期估计为6个月。 尤尼斯·肯尼迪·施莱弗国家医院内部人群健康研究分部 儿童健康与人类发展研究所由三个分支机构组成：1) 流行病学处；2)生物统计和生物信息学处；3)预防研究 分支机构[http://www.nichd.nih.gov/about/org/despr/].该司的核心任务是 设计、实施和指导流行病学、行为和生物统计学研究 专注于生殖、围产期和儿科流行病学研究以及研究 解决儿童和青少年的健康行为。 该司此前曾从#年开始进行预防子痫前期的钙剂试验 1992-1995年间，在美国的5家诊所测试补钙对风险和 子痫前期(PE)的严重程度。CPEP的一个研究地点(田纳西州孟菲斯)进行了一项 “胎儿骨矿化”的辅助研究，母亲和她的新生儿都可以 接受双能X射线吸收法(DXA)扫描。多项研究表明。 怀孕期间维生素D缺乏的妇女生下的婴儿往往出生体重较低， 尽管证据仍有争议。为了在之前发现的基础上，我们将测量 孕妇CPEP血清中维生素D生物标志物检测对孕妇维生素D状况的影响 与新生儿体脂和新生儿骨矿含量相关。母体维生素D 还将结合母亲的骨矿含量来检查其状况。血清样本 在这项试验中纵向收集的数据将在整个试验过程中提供几种维生素D状态的衡量标准 这使我们能够更好地描述这些女性的维生素D特征。这些 还将使用措施来表征维生素D生物标记物的轨迹 白人和非裔美国人两个种族群体的妊娠分层。血清样本 已存储在NICHD生物信息库中，温度为-70℃。 参与者将被送往实验室

The tasks to utilize this contract are to analyze samples from the Calcium for Preeclampsia Prevention trial (CPEP) to measure total 25(0H)D, its isoforms (25(0H)D2 and 25(0H)D3), Vitamin D binding protein (VDBP), albumin, and total calcium in up to 992 serum samples from the CPEP trial. Period of performance for this task is estimated to be 6 months. The Division of lntramural Population Health Research of the Eunice Kennedy Shriver National Institute of Child Health and Human Development encompasses three branches: 1) Epidemiology Branch; 2) Biostatistics and Bioinformatics Branch and 3) Prevention Research Branch [http://www.nichd.nih.gov/about/org/despr/]. Central to the Division's mission is to design, implement, and direct epidemiologic, behavioral and biostatistical research which focuses on reproductive, perinatal and pediatric epidemiologic research as well as research addressing child and adolescent health behaviors. The Division previously conducted the Calcium for Preeclampsia Prevention (CPEP) trial from 1992-1995 across 5 clinics in the US testing the effect of calcium supplementation on risk and severity of preeclampsia (PE). One of the CPEP study sites (Memphis, TN) conducted an ancillary study of' Fetal bone mineralization' whereby both the mother and her newborn could receive a dual-energy X-ray absorptiometry (DXA) scan. Several studies have shown that . women with vitamin D deficiency during pregnancy tend to have infants with lower birth weight, although the evidence is still controversial. To build on previous findings, we will measure maternal CPEP serum samples for vitamin D biomarkers to investigate maternal vitamin D status in association with neonatal body fat and neonatal bone mineral content. Maternal vitamin D status will also be examined in association with maternal bone mineral content. Serum samples collected longitudinally in this trial will offer several measures of vitamin D status throughout gestation enabling us to better characterize the vitamin D profile of these women. These measures will also be used to characterize the trajectories of vitamin D biomarkers across gestation stratifying by two racial groups namely Whites and African-Americans. Serum samples from the trial have been stored in the NICHD Bio-repository at -70¿C. Aliquots of the participants will be shipped to the laboratory