Fluidigm BioMark HD MX/HX Real-Time PCR System

描述(由申请人提供)： 加州大学戴维斯分校将购买BioMark“HD MX/HX系统(Fluidigm Corporation)，该系统可执行单细胞表达分析、有限RNA样本的表达分析、数字聚合酶链式反应/拷贝数变异、单倍型、基因分型、单核苷酸多态突变检测和微阵列验证。该系统在化学方面非常灵活(例如，TaqMan(R)、EvaGreen[SYBR]、罗氏UPL、等位基因特异性分析、Solaris等)，允许用户快速调整现有的分析以适应这一高度敏感的平台。最先进的Fluidigm BioMark“HD MX/HX系统由一个实时聚合酶链式反应仪器、用于多路样品和试剂的动态阵列集成流体电路(IFC)以及一个IFC加载器组成。 动态数组IFC和读取器，用于量化和记录结果。该仪器将帮助我们实现加快基础、翻译和临床研究中生物医学发现速度的目标，这是加州大学戴维斯分校卫生系统和美国国立卫生研究院的共同目标。目前，加州大学戴维斯分校的22笔现役NIH补助金和1笔额外的校外补助金将受益于该仪器，NIH支持的研究项目将使用超过90%的总使用时间。仪器将安装和维护在校园的光学生物学/流式细胞术资源核心(Opticore)设施中，在那里将仔细维护和正确使用，调查人员将最大限度地接触到它。最初，该仪器将用于研究项目，重点是对全球健康具有重要意义的疾病(例如疟疾、艾滋病毒和结核病)、慢性病(例如癌症和神经生物学)和新的治疗方法(例如癌症干细胞生物学、免疫疗法和药物疗法)。加州大学戴维斯分校已经确立了安装、维护和优化仪器的机构承诺。一个内部咨询委员会将对仪器进行监督，以确保向研究界提供仪器的短期和长期使用和服务。

DESCRIPTION (provided by applicant): UC Davis will purchase a BioMark" HD MX/HX System (Fluidigm Corporation) that performs single cell expression analysis, expression analysis from limited RNA samples, digital PCR/copy number variation, haplotyping, genotyping, single nucleotide polymorphism mutation detection, and microarray validation. The system is extremely flexible with regard to chemistry (e.g., TaqMan(R), EvaGreen[SYBR], Roche UPL, allele specific assays, Solaris, etc), allowing users to quickly adapt existing assays to this highly sensitive platform. The state-of-the-art Fluidigm BioMark" HD MX/HX System consists of a real-time PCR instrument, Dynamic Array" integrated fluidic circuits (IFCs) for multiplexing samples and reagents, as well as an IFC loader to load the Dynamic Array IFCs and a Reader to quantify and record results. The instrumentation will help us to meet our goal to accelerate our rate of biomedical discoveries in basic, translational, and clinical research, which are shared goals of the UC Davis Health System and the NIH. Currently, 22 active NIH grants and one additional extramural grant based at UC Davis will benefit from the instrumentation, which will be used by NIH-supported research programs more than 90% of the total usage time. The instrumentation will be installed and maintained in the Optical Biology/Flow Cytometry Resource Core (Opticore) facility on campus, where it will be carefully maintained, correctly used, and investigators will have maximum access to it. Initially, the instrumentation will be used by research programs with a focus on diseases of global health importance (e.g., malaria, HIV and tuberculosis), chronic diseases (e.g., cancer and neurobiology), and new therapeutic approaches (e.g., cancer stem cell biology, immunotherapeutics and pharmacotherapy). UC Davis has established the institutional commitment to install, maintain and optimize the instrumentation. An internal Advisory Committee will provide oversight for the instrumentation to ensure the short- and long-term access and service of the instrument to the research community.