Fluidigm BioMark HD MX/HX Real-Time PCR System

Fluidigm BioMark HD MX/HX 实时 PCR 系统

• 批准号: 8446862
• 负责人: Shirley Luckhart
• 金额: $ 26.12万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2014-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8446862
• 关键词: Advisory Committees; Alleles; Basic Science; Biological Assay; Biology; Cells; Chemistry; Chronic Disease; Clinical Research; Communities; Copy Number Polymorphism; Core Facility; Disease; Ensure; Extramural Activities; Flow Cytometry; Genotype; Goals; Grant; HIV; Health system; Immunotherapeutic agent; Malaria; Malignant Neoplasms; Mutation Detection; N.I.H. Research Support; Neurobiology; Optics; Pharmacotherapy; RNA; Reader; Reagent; Research; Research Personnel; Resources; Sampling; Services; Single Nucleotide Polymorphism; System; Time; Translational Research; Tuberculosis; United States National Institutes of Health; Validation; base; cancer stem cell; digital; flexibility; global health; instrument; instrumentation; meetings; novel therapeutic intervention; programs; stem cell biology

DESCRIPTION (provided by applicant): UC Davis will purchase a BioMark" HD MX/HX System (Fluidigm Corporation) that performs single cell expression analysis, expression analysis from limited RNA samples, digital PCR/copy number variation, haplotyping, genotyping, single nucleotide polymorphism mutation detection, and microarray validation. The system is extremely flexible with regard to chemistry (e.g., TaqMan(R), EvaGreen[SYBR], Roche UPL, allele specific assays, Solaris, etc), allowing users to quickly adapt existing assays to this highly sensitive platform. The state-of-the-art Fluidigm BioMark" HD MX/HX System consists of a real-time PCR instrument, Dynamic Array" integrated fluidic circuits (IFCs) for multiplexing samples and reagents, as well as an IFC loader to load the Dynamic Array IFCs and a Reader to quantify and record results. The instrumentation will help us to meet our goal to accelerate our rate of biomedical discoveries in basic, translational, and clinical research, which are shared goals of the UC Davis Health System and the NIH. Currently, 22 active NIH grants and one additional extramural grant based at UC Davis will benefit from the instrumentation, which will be used by NIH-supported research programs more than 90% of the total usage time. The instrumentation will be installed and maintained in the Optical Biology/Flow Cytometry Resource Core (Opticore) facility on campus, where it will be carefully maintained, correctly used, and investigators will have maximum access to it. Initially, the instrumentation will be used by research programs with a focus on diseases of global health importance (e.g., malaria, HIV and tuberculosis), chronic diseases (e.g., cancer and neurobiology), and new therapeutic approaches (e.g., cancer stem cell biology, immunotherapeutics and pharmacotherapy). UC Davis has established the institutional commitment to install, maintain and optimize the instrumentation. An internal Advisory Committee will provide oversight for the instrumentation to ensure the short- and long-term access and service of the instrument to the research community.

描述（由申请人提供）： 加州大学戴维斯分校将购买BioMark”HD MX/HX系统（Fluidigm Corporation），该系统可进行单细胞表达分析、有限RNA样本的表达分析、数字PCR/拷贝数变异、单体型分析、基因分型、单核苷酸多态性突变检测和微阵列验证。该系统在化学方面非常灵活（例如，TaqMan（R）、EvaGreen[SYBR]、Roche UPL、等位基因特异性测定、Solaris等），使用户能够快速地将现有的测定适应于这种高度敏感的平台。最先进的Fluidigm BioMark”HD MX/HX系统由实时PCR仪器、用于多路复用样品和试剂的“动态阵列”集成流控回路（IFC）以及用于加载样品的IFC加载器组成。 动态阵列IFC和一个读取器来量化和记录结果。仪器将帮助我们实现我们的目标，加快我们在基础，转化和临床研究中的生物医学发现速度，这是加州大学戴维斯分校卫生系统和NIH的共同目标。目前，22个活跃的NIH赠款和一个额外的校外赠款基于加州大学戴维斯分校将受益于仪器，这将是由NIH支持的研究计划使用超过90%的总使用时间。仪器将安装和维护在校园的光学生物学/流式细胞术资源核心（Opticore）设施中，在那里它将得到仔细维护，正确使用，研究人员将最大限度地使用它。最初，仪器将用于研究项目，重点关注全球健康重要性疾病（例如，疟疾、艾滋病毒和结核病），慢性疾病（例如，癌症和神经生物学），以及新的治疗方法（例如，癌症干细胞生物学、免疫疗法和药物疗法）。加州大学戴维斯分校已经建立了安装，维护和优化仪器的机构承诺。一个内部咨询委员会将对仪器进行监督，以确保研究界能够短期和长期使用仪器并为其提供服务。