IVIS Spectrum small animal imaging system

描述（由申请人提供）：儿童医院奥克兰研究所（CHORI）是一个独立的研究所，作为儿童医院和研究中心奥克兰的研究部门。我们的研究项目是美国国家卫生研究院（NIH）资助的顶级儿科研究部门之一。然而，我们目前缺乏小动物成像能力来支持涉及啮齿动物的临床前研究。该提案要求提供资金，用于购买[IVIS Spectrum]供CHORI使用，以促进涉及小动物成像的各种项目，包括监测肿瘤异种移植物和病原微生物的生长，并跟踪标记有荧光或发光标记的治疗剂的生物利用度。其他新的研究将采用光学成像方法来跟踪LacZ转基因小鼠模型中的基因表达。12个主要用户，20个NIH资助的肿瘤生物学，传染病，纳米技术和其他生物医学重要领域的项目将立即受益于该仪器。此外，CHORI对IVIS Spectrum的收购可能会为许多其他NIH资助的研究人员提供间接利益，这些研究人员可以通过在我们参与敲除小鼠项目（KOMP）的主要用户之一的指导下有效生产和分型小鼠疾病模型。要求的仪器系统提供全套IVIS光学功能[包括]平面成像、光谱分离、3D生物发光和3D荧光。该系统被配置为提供成像信息[使我们能够]监测引入啮齿动物的细胞。用生物发光或荧光标记物标记的癌细胞或微生物细胞可以被监测，以使用光谱成像系统检测总体积和侵袭的变化。CHORI没有现有的小动物成像系统。将啮齿动物运输到北方加州湾区的其他地点是不可行的，因为那里有小动物成像系统。目前，病理学检查以确定肿瘤进展或细菌侵袭是现场唯一可用的方法。这种方法耗时，需要使用许多动物来跟踪疾病随时间的进展。CHORI研究人员目前正在通过与能够使用小动物成像仪器的科学家的合作安排进行小动物成像。IVIS Spectrum [是]一款首屈一指的光学成像系统[和]一款易于操作的多功能仪器，我们的用户群对此非常熟悉。因此，我们从市场上众多的竞争成像系统中选择了这一系统作为最佳系统。成像系统将大大推进目前的项目，并增加共用设备。

DESCRIPTION (provided by applicant): Children's Hospital Oakland Research Institute (CHORI) is a free-standing institute that serves as the research arm of the Children's Hospital & Research Center Oakland. Our research program is among the top pediatric research departments in the nation with regard to funding from the National Institutes of Health (NIH). However, we currently lack small animal imaging capabilities to support preclinical research involving rodents. This proposal requests funds to purchase an [IVIS Spectrum] for use at CHORI to facilitate a variety of projects involving small animal imaging, including to monitor the growth of tumor xenografts and pathogenic microbes and to follow the bioavailability of therapeutic agents labeled with fluorescent or luminescent markers. Additional novel studies will employ optical imaging approaches to follow gene expression in LacZ transgenic mouse models. Twelve major users, 20 NIH-funded projects in tumor biology, infectious disease, nanotechnology and other areas of biomedical importance will immediately benefit from this instrument. Additionally, CHORI's acquisition of the [IVIS Spectrum] would likely provide indirect benefit to many other NIH-funded investigators via the efficient production and phenotyping of murine models of disease generated under the direction of one of our major users participating in the Knock Out Mouse Project (KOMP). The requested instrument system provides the full suite of IVIS optical features [including] Planer Imaging, Spectral Unmixing, 3D bioluminescence and 3D fluorescence. This system is configured to provide imaging information [that allows us] to monitor cells introduced into a rodent. Cancer cells or microbial cells labeled with bioluminescent or fluorescent markers can be monitored to detect changes in total volume and invasion using the Spectrum imaging system. CHORI has no existing small animal imaging system. Transport of rodents to other sites in the Bay Area of Northern California where small animal imaging systems are available is not feasible. Currently, pathological examination to identify tumor progression or bacterial invasiveness is the only method available on site. This approach is time-consuming and requires the use of many animals to follow disease progression over time. CHORI investigators undertaking small animal imaging are currently doing so through collaborative arrangements with scientists who have access to small animal imaging instrumentation. The IVIS Spectrum [is] a premiere optical imaging system [and] a versatile instrument that is easy to operate and with which our user group is highly familiar. Thus, we have chosen this as the optimal system from a number of competing imaging systems on the market. The imaging system will significantly advance the current projects and add to the pool of shared equipment.