EPR Detection of Free Radicals in Vascular Disease: Bruker EMXPlus Spectrometer

EPR 检测血管疾病中的自由基：布鲁克 EMXPlus 光谱仪

• 批准号: 8447255
• 负责人: Eric Eugene Kelley
• 金额: $ 30.4万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-15 至 2014-06-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447255
• 关键词: Biological Models; Cell Membrane Permeability; Cell membrane; Detection; Development; Disease; Disease Progression; Drug Interactions; Electron Spin Resonance Spectroscopy; Fingerprint; Free Radicals; Goals; Gold; In Vitro; Inferior; Institution; Measures; Medical Research; Methodology; Nitrogen; Oxidation-Reduction; Proteins; Reactive Oxygen Species; Research; Research Personnel; Source; Specificity; Spin Trapping; System; Techniques; Technology; Tissues; Toxic effect; Universities; Vascular Diseases; biological systems; in vivo; medical schools; novel strategies; success

DESCRIPTION: Reactive species of oxygen and nitrogen are becoming increasingly appreciated for their impact on the onset and progression of disease. It is therefore imperative for medical research institutions to possess robust free radical research technology. Currently, investigators at the University of Pittsburgh, School of Medicine are relegated to the use of inferior methodologies to critically interrogate free radical species in their model systems. For example, all currently available fluorescent, colorimetric, luminescent and chemiluminescent probes used to detect free radical species demonstrate reproducible results with relatively high sensitivity however, they have significant limitations regarding specificity, the propensity to redox cycle, membrane permeability and toxicity. This being noted, electron paramagnetic resonance (EPR) spectroscopy is the only technique currently available that affords direct identification of free radicals in biological systems and as such remains the gold standard for free radical research. The utility of EPR is exemplified in its capacity to provide specific fingerprint spectra that identify the free radical being measured. Thus, EPR can provide direct detection and identification of stable free radical species. For less stable free radical species, EPR spin trapping techniques permit detection and identification in a multitude of in vitro and in vivo systems. This technique has proven extremely informative for investigating formation and identifying sources of radicals in cellular, tissue and in vivo systems as well as investigating protein radical intermediates and studying drug interactions with cell membranes. To date, investigators in the School of Medicine have limited to no access to this technology. As such, acquisition of an EPR spectrometer would serve to uniquely cultivate the development of current research initiatives, incentivize new collaborative efforts and enhance the potential for success in achieving our overarching goal of developing novel strategies to treat disease.

描述：活性氧和氮的物种越来越受到重视，因为它们对疾病的发生和进展有影响。因此，医学研究机构必须拥有强大的自由基研究技术。目前，匹兹堡大学医学院的研究人员在他们的模型系统中使用低劣的方法来批判性地询问自由基物种。例如，用于检测自由基物质的所有目前可用的荧光、比色、发光和荧光发光探针都显示出具有相对高灵敏度的可再现结果，然而，它们在特异性、氧化还原循环倾向、膜渗透性和毒性方面具有显著的局限性。值得注意的是，电子顺磁共振（EPR）光谱是目前唯一可用的技术，提供直接识别生物系统中的自由基，因此仍然是自由基研究的金标准。EPR的实用性体现在其提供识别被测量的自由基的特定指纹光谱的能力。因此，EPR可以提供稳定的自由基物种的直接检测和鉴定。对于不太稳定的自由基种类，EPR自旋捕获技术允许在大量的体外和体内系统中检测和鉴定。这种技术已被证明是非常翔实的调查形成和确定来源的自由基在细胞，组织和体内系统，以及调查蛋白质自由基中间体和研究药物与细胞膜的相互作用。到目前为止，医学院的研究人员对这项技术的使用受到限制。因此，购买EPR光谱仪将有助于独特地培养当前研究计划的发展，激励新的合作努力，并提高成功实现我们开发新的疾病治疗策略的总体目标的潜力。