Leica LMD7000- Laser Microdissection System

描述（由申请人提供）：本SIG提案要求获得223,777美元的支持，用于购买徕卡LMD7000激光显微解剖系统。该仪器将取代我们现有的，无法修复的损坏的大角星系统。新仪器将安装在凯斯综合癌症中心的一个核心设施中，为癌症研究人员和更广泛的生物医学研究界提供服务。所要求的仪器将用于从通常构成活检材料的复杂背景的异质组织中特异性地定位和获取接近100%的同质组织样本。我们的LCM设施主管在操作LCM系统和从LCM采购的材料中提取RNA/DNA以及进一步处理这些材料用于基因组和/或蛋白质组学研究方面拥有5年的经验。一个由10名研究人员组成的小组，在nih资助的总额超过1600万美元的资助下，将利用所要求的LCM系统。一个突出的资助是《孢子》的资助，总直接成本为730万美元。各种各样的研究，如结肠癌、巴雷特食管（一种癌前疾病）、皮肤/牛皮癣、视网膜和小细胞肺癌微环境，目前在选择特定细胞群进行高水平基因组分析和/o蛋白质组学分析的能力方面受到严重限制。所要求的LCM系统将使这些人能够完成他们的研究。我们比较了4个LCM平台的价格/功能，并将其缩小到两个系统，Leica和Molecular Machines and Industries (MMI)，以便现场进行实际评估。徕卡LMD7000是我们明确的首选，以满足Case nih资助社区的需求。销售/技术人员有提供全面有效的技术支持的历史。机构支持是广泛的，包括LCM专用的房间，相邻实验室的工作台空间。主管的工资支持（20%的努力）和助理培训师的工资支持（10%的努力）。署长会定期与谘询委员会举行会议，谘询委员会成员包括已获授权的LCM使用者。本建议中概述的项目需要收集特定的细胞类型用于

DESCRIPTION (provided by applicant): This SIG proposal requests support in the amount of $223,777 to purchase a Leica LMD7000 Laser Micro-Dissection system. The instrument will replace our existing, irreparably damaged Arcturus system. The new instrument will be set up in a core facility at Case Comprehensive Cancer Center serving both cancer researchers and the broader biomedical research community. The requested instrument will be used to specifically target and procure near-100% homogenous samples of tissue from complex backgrounds of heterogenous tissues that typically make up biopsy materials. Our LCM facility director has 5 years experience in operating an LCM system and extracting RNA/DNA from LCM-procured material and further processing these materials for genomic and/or proteomic studies. A group of 10 investigators, with NIH-funded grants totaling more than $16M, will utilize the requested LCM system. One stand-out grant is a SPORE grant with $7.3M in total direct costs. A diverse set of studies such as: Colon cancer, Barrett's Esophagous (a pre-cancerous condition), skin/Psoriasis, Retina and small-cell lung cancer microenvironment, are currently severely limited in their ability to select specific cell populations for high level genomic profiling and/o proteomic analyses. The requested LCM system will enable these individuals to complete their research. We compared prices/features across 4 LCM platforms and narrowed it down to two systems, Leica and Molecular Machines and Industries (MMI), to bring on site for hands-on evaluations. The Leica LMD7000 is our clear preference for meeting the needs of Case NIH-funded community. The sales/technical people have a history of providing full and effective technical support. Institutional support is broad and includes a dedicated room for LCM, bench space in an adjacent lab., salary support (20% effort) for a Director and (10% effort) for an assistant trainer. The director will meet regularly with an Advisory Committee comprising vested LCM users. The projects outlined in this proposal require the collection of specific cell types for downstream molecular biology. Of particular value for RNA work, is the "no touch" manner in which tissues are collected by the Leica LMD7000. Users will benefit from close interaction with other excellent existing shared resources in gene expression analysis, sequencing and bioinformatics in a highly integrated university-wide system of shared resources.