Pooled shRNA screens for target discoveries in cancer

混合 shRNA 筛选癌症靶点发现

• 批准号: 8763525
• 负责人: Ji Luo
• 金额: $ 13.22万
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8763525
• 关键词: Area; CCR; Cancer cell line; Dependency; Disease; Genes; Genetic; Goals; Human; Individual; Intramural Research Program; Lead; Libraries; Malignant Neoplasms; Methods; Molecular Target; Normal Cell; RNA Interference; Research Personnel; Resources; Technology; United States National Institutes of Health; Validation; cancer cell; cost effective; follow-up; small hairpin RNA; tool

BACKGROUND. RNAi is a powerful tool for dissecting genetic interactions. We have developed technologies to conduct rapid and cost-effective shRNA library screens to identify genetic dependencies in cancer cells. PURPOSE. In this project we will collaborate with other investigators (primarily investigators in the NIH Intramural Program) to enable them to conduct shRNA screens in cancer cell lines. SIGNIFICANT MATERIALS AND METHODS. Pooled shRNA library targeting most annotated human genes. Individual shRNA clones for follow up hit validation and mechanism studies. FY2013 ACCOMPLISHMENT. We are currently engaged in a number of shRNA screens in difference cancer disease areas.

背景。RNAi是分析基因相互作用的有力工具。我们已经开发了技术来进行快速和经济有效的shRNA文库筛选，以确定癌细胞中的遗传依赖性。目的。在这个项目中，我们将与其他研究人员（主要是NIH校内项目的研究人员）合作，使他们能够在癌细胞系中进行shRNA筛选。重要的材料和方法。汇集的shRNA文库针对大多数注释的人类基因。用于后续命中验证和机制研究的单个shRNA克隆。FY2013成就。我们目前在不同的癌症疾病领域从事许多shRNA筛选。