Genetically-Encoded Tools for Manipulation of Ion Channel and Receptor Functions

用于操纵离子通道和受体功能的基因编码工具

• 批准号: 8459881
• 负责人: Edward S. Boyden
• 金额: $ 32.33万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8459881
• 关键词: AMPA Receptors; Accounting; Adoption; Adverse effects; Affect; Anxiety; Area; Behavior; Behavioral; Biology; Brain; Brain Diseases; Calcium Channel; Cells; Chemicals; Chimeric Proteins; Chloride Ion; Chlorides; Clinical; Complex; Critiques; Custom; Data; Development; Diabetes Mellitus; Disease; Disease model; Drug Addiction; Drug Targeting; Engineering; Epilepsy; Evaluation; Fostering; Funding; Funding Opportunities; Future; GABA Receptor; Halorhodopsins; Hypertension; Individual; Institutes; Ion Channel; Ions; Life; Light; Mediating; Memory; Mental disorders; Methodology; Mission; Molecular; Movement; Mus; Mutagenesis; N-Methyl-D-Aspartate Receptors; Nervous system structure; Neuroglia; Neuromodulator; Neurons; Neurosciences; Neurotransmitter Receptor; Opsin; Optics; Pathology; Pathway interactions; Performance; Pharmaceutical Preparations; Physiologic pulse; Physiological; Physiological Processes; Pilot Projects; Play; Potassium; Potassium Channel; Probability; Prosthesis; Protein Engineering; Proteins; Proton Pump; Pump; Reagent; Research; Risk; Role; Schizophrenia; Science; Sleep Disorders; Sodium; Sodium Channel; Speed; Structure; Study Section; Supplementation; Technology; Testing; Therapeutic; Toxin; United States National Institutes of Health; Update; Vertebral column; Writing; base; cell type; chronic pain; clinically relevant; design; high throughput screening; in vivo; information processing; innovation; meetings; millisecond; nervous system disorder; novel; receptor; receptor function; relating to nervous system; tool; voltage

DESCRIPTION (provided by applicant): Ion channels and neurotransmitter receptors are amongst the most important molecules in the nervous system. They support the high-speed physiological processes that enable neurons to function, they are implicated in many neurological and psychiatric disorders, and they present an incredibly important set of drug targets for treating these diseases. In order to enable a better understanding of how specific ion channels and receptors contribute to behaviors and pathologies, we propose to engineer a toolbox of fully genetically encoded reagents that, when expressed in specific neurons in the brain, enable specific ion channels and ionotropic neurotransmitter receptors to be driven or blocked in a temporally precise fashion, using pulses of light. We anticipate that these tools will find widespread use in both basic and clinical neuroscience, and in other fields of biology, for revealing the roles that specific ion channels and receptors (or changes in their activity levels) play in neural computations, behaviors, and disease states, and for revealing more specific drug targets.

描述（由申请人提供）：离子通道和神经递质受体是神经系统中最重要的分子之一。它们支持使神经元发挥作用的高速生理过程，它们与许多神经系统和精神疾病有关，并且提出了一组非常重要的治疗这些疾病的药物靶标。为了更好地理解特定的离子通道和受体如何对行为和病理的贡献，我们建议设计一个完全遗传编码的试剂的工具箱，当在大脑中表达特定的神经元时，启用特定的离子通道和离子神经脱落的受体，以使其在pulsies pulsise中驱动或封闭。我们预计，这些工具将在基本和临床神经科学以及其他生物学领域都发现广泛使用，以揭示特定离子通道和受体（或活动水平的变化）在神经计算，行为和疾病状态以及揭示更特定的药物靶标的作用。