High-Performance Imaging Through Scattering Living Tissue

通过散射活组织进行高性能成像

• 批准号: 9978808
• 负责人: Edward S. Boyden
• 金额: $ 83.96万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9978808
• 关键词: Action Potentials; Attention; Biological; Biology; Brain; Brain Diseases; Brain Pathology; Brain imaging; Cells; Cellular Membrane; Chemicals; Clinical; Code; Communities; Complex; Custom; Cytosol; Development; Diagnostic; Disease; Emotions; Event; Excision; Extracellular Space; Fluorescent Probes; Functional Imaging; Goals; Image; Immunity; Laser Scanning Microscopy; Lipids; Malignant Neoplasms; Maps; Medicine; Nature; Neurons; Neurosciences; Operative Surgical Procedures; Optics; Organelles; Pattern; Performance; Photons; Physiological; Process; Proteins; Refractive Indices; Research; Resource Sharing; Safety; Scanning; Speed; Synaptic Transmission; Technology; Three-Dimensional Imaging; Tissues; adaptive optics; aqueous; brain tissue; brain volume; chemical synthesis; commercialization; design; efficacy testing; extracellular; high risk; imaging modality; improved; in vitro testing; in vivo evaluation; innovation; insight; interest; light scattering; material transfer agreement; millimeter; millisecond; nanoparticle; neural circuit; neural patterning; new technology; relating to nervous system; small molecule; tool; two-photon; vector

The ability to dynamically image, using fluorescent probes, neural activity and other fast physiological events in living brains has begun to revolutionize neuroscience. The fundamental limitation of optical scattering in living tissue, which limits fast imaging to shallow depths, has attracted much attention from hardware inventors, who have developed a diversity of strategies -- ranging from multiphoton laser-scanning microscopy, to adaptive optics approaches that attempt to invert tissue scattering. However, imaging extended volumes of brain tissue, at rates that keep up with fast events like action potentials, remains a challenge. We here propose to invert the problem, and make the living brain, itself, more transparent. By developing chemicals that safely and efficaciously smooth out refractive index inhomogeneities that scatter light, we will enable observation of high speed neural processes throughout extended volumes, e.g. entire cortical microcircuits (and potentially, across arbitrary scales). In this way, neuroscientists will be able to analyze the neural activity patterns across circuits underlying complex phenomena like emotions, decisions, and actions, and that contribute to disease states. Beyond neuroscience, our technology may broadly improve the observation of high-speed physiological events in the living body, of importance to immunity, development, cancer, and other parts of biology and medicine.

能够动态成像，利用荧光探针，快速观察神经活动等生理事件

项目成果

Distance-dependent resonance energy transfer in alkyl-terminated Si nanocrystal solids.

烷基封端硅纳米晶体固体中距离依赖的共振能量转移。

• DOI: 10.1063/5.0079571
• 发表时间: 2022
• 期刊: The Journal of chemical physics
• 作者: Li,Zhaohan;Robinson,ZacharyL;Elvati,Paolo;Violi,Angela;Kortshagen,UweR
• 通讯作者: Kortshagen,UweR

Aerosol-Phase Synthesis and Processing of Luminescent Silicon Nanocrystals.

发光硅纳米晶体的气溶胶相合成和加工。

• DOI: 10.1021/acs.chemmater.9b02743
• 发表时间: 2019
• 期刊: Chemistry of materials : a publication of the American Chemical Society
• 作者: Li,Zhaohan;Kortshagen,UweR
• 通讯作者: Kortshagen,UweR

Synthesis of PEG-grafted boron doped Si nanocrystals.

• DOI: 10.1063/1.5128608
• 发表时间: 2019-12
• 期刊: The Journal of chemical physics
• 作者: Jesse R Greenhagen;Himashi P. Andaraarachchi;Zhaohan Li;U. Kortshagen