Molecular Biology

莫奈尔研究人员的主要专业范围很广：分析化学和化学生态学(博士。Kimball， Smith和Prefi)，生物化学和生物物理学(dr。Brand和Margolskee)，分子和细胞生物学(dr。Huang， Margolskee和Wang)，遗传学和神经科学(dr。Bachmanov, Beauchamp, Gelperin, Lowe, Reed, Reisert， Tordoff和Yamazki)，内分泌学和生理学(dr。Friedman, Margolskee, Teff, Wysocki, and Zhao)，心理物理学(dr。Breslin, Dalton, Lundstrom, Mennella, Pelchat, and Wise)，以及临床研究(dr。考特和特夫)。许多实验室缺乏分子生物学的基本专业知识，但仍希望在其研究中利用分子生物学技术。雇用分子技术熟练的技术人员或博士后可以解决这些需求，但考虑到化学意义上的许多问题所带来的多种技术，聘请单个实验室成员来解决每种所需的技术是不现实的。此外，将生成模型生物所需的所有分子生物学步骤外包出去将是相当昂贵的。莫奈尔大学更常见的解决方案是与具有分子生物学专业知识的同事合作。事实上，在莫奈尔，合作是非常普遍的，鉴于该中心对化学感官的关注，双方的兴趣是频繁的。然而，往往我们的努力

The primary expertise of Monell investigators ranges broadly: analytical chemistry and chemical ecology (Drs. Kimball, Smith, and Prefi), biochemistry and biophysics (Drs. Brand and Margolskee), molecular and cellular biology (Drs. Huang, Margolskee, and Wang), genetics and neuroscience (Drs. Bachmanov, Beauchamp, Gelperin, Lowe, Reed, Reisert, Tordoff, and Yamazki), endocrinology and physiology (Drs. Friedman, Margolskee, Teff, Wysocki, and Zhao), psychophysics (Drs. Breslin, Dalton, Lundstrom, Mennella, Pelchat, and Wise), and clinical research (Drs. Cowart and Teff). Many of these laboratories lack primary expertise in molecular biology but still wish to utilize molecular biological techniques in their research. Hiring molecularly skilled technical staff or postdocs can address these needs, but given the multiple techniques brought to bear on many problems in the chemical senses, it is not practical to hire individual lab members to address each needed technique. Also, it would be quite costly to contract out all the molecular biological steps needed to generate a model organism. A more common solution at Monell is to enter into collaborations with colleagues that have molecular biology expertise. Indeed, collaborations are very common at Monell, and given the Center's focus on chemical senses, mutual interest is frequent. However, often the efforts of our molecular biologists are already fully committed to other projects. Thus, having a Molecular Biology Core to provide these specialized services on an as-needed basis and to train staff in such techniques will address ongoing needs in many of our laboratories. This Core will provide a central resource of experience in multiple molecular biological techniques that would be inefficient to replicate in each individual laboratory, especially those for which molecular biology is not a primary focus. In addition, centralized purchasing of the reagents and equipment needed for molecular biology is more cost-effective than multiple independent purchases by several labs. Given the costly and exacting nature of work with transgenic and knockout mice, it is important that the steps to generate engineered model organisms be done accurately and efficiently. This Core will enable PIs with no prior knowledge or expertise in molecular biology to produce genetically modified animal models and cell lines with expert assistance in reasonable time and for a reasonable cost. Of the 26 current Faculty research groups at Monell, 22 (85%) will benefit from the molecular approaches provided by this Core. Of the 13 R01 grants in our Research Base, 10 (77%)) will use the molecular approaches and services the Core will provide. The availability of Core services extends the capabilities of the participating laboratories beyond the nidus of each laboratory's own capabilities.