Protein Mass Spectrometry Core Facility for Neuroscience

描述（由申请人提供）：自2005年成立以来，纽约大学神经科学蛋白质质谱核心设施为纽约大学医学院的分子神经科学家提供了基于质谱的尖端蛋白质分析。这项拨款的目的是为纽约大学的神经科学家提供持续的资金，使他们能够利用复杂而复杂的蛋白质质谱法和蛋白质组学，否则他们将无法获得这些技术，并保证他们能够获得这些技术。拟议的神经科学蛋白质质谱核心设施将使NINDS和其他NIH资助的纽约大学神经科学家能够识别一种到数千种感兴趣的蛋白质，表征这些蛋白质的翻译后修饰，如磷酸化、糖基化、蛋白质水解和脂质修饰，进行功能蛋白质组学研究，以识别神经元中关键信号转导过程中涉及的蛋白质。通过表面等离子体共振表征蛋白质-蛋白质相互作用，并进行自动RNAi实验。该核心设施将极大地受益于神经生长和再生、神经肌肉连接形成、癌症转移、轴突引导、轴突结构域组装、病变诱导的突触可塑性、钾通道功能、阿尔茨海默氏症和其他淀粉样疾病、大脑发育、衰老、脑癌和神经递质受体运输等研究领域。

DESCRIPTION (provided by applicant): Since its establishment in 2005, the New York University Protein Mass Spectrometry Core Facility for neuroscience has provided cutting edge mass spectrometry-based protein analysis to molecular neuroscientists at the New York University School of Medicine. The purpose of this grant is to provide continued funding to enable neuroscientists at NYU to take advantage of sophisticated and involved protein mass spectrometry and proteomics that would otherwise not be available to them, and guarantee them access to the technology. The proposed Protein Mass Spectrometry Core Facility for Neuroscience would enable NINDS- and other NIH- funded neuroscientists at New York University to identify from one to thousands of proteins of interest, to characterize posttranslational modifications of these proteins such as phosphorylation, glycosylation, proteolysis and modification by lipids, to perform functional proteomics studies to identify the proteins involved in key signal transduction processes in neurons, to characterize protein-protein interactions by surface plasmon resonance and to perform automated RNAi experiments. Research areas that would greatly benefit from this core facility include nerve growth and regeneration, neuromuscular junction formation, cancer metastasis, axon guidance, axon domain assembly, lesion induced synaptic plasticity, potassium channel function, Alzheimer's and other amyloid diseases, brain development, aging, brain cancer, and neurotransmitter receptor trafficking. RELEVANCE: The NYU Mass Spectrometry Core Facility for Neuroscience ensures that neuroscientists have access to cutting edge mass spectrometry technology to enable their basic research in such key areas as nerve growth and regeneration, neuromuscular disorders, cerebral hemorrhagic stroke, nervous system development, synaptic plasticity, affective disorders such as depression, Alzheimer's and other amyloid diseases, and aging.