LTQ-Orbitrap Mass Spectrometer.

LTQ-Orbitrap 质谱仪。

• 批准号: 8052013
• 负责人: Thomas A Neubert
• 金额: $ 60万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2012-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8052013
• 关键词: Cells; Clinical; Core Facility; Detection; Development; Disease; Electrons; Ensure; Funding; Institutes; Malignant Neoplasms; Mass Spectrum Analysis; Minor; Molecular; Nerve Degeneration; Neuromuscular Diseases; Neurons; Neurosciences; Peptides; Protein Analysis; Proteins; Proteomics; Relative (related person); Research; Signal Transduction; Stable Isotope Labeling; Technology; United States National Institutes of Health; Woman; biomedical scientist; instrument; mass spectrometer; medical schools; research study

DESCRIPTION (provided by applicant): The NYU Mass Spectrometry Core Facility for Neuroscience, the 100 Women in Hedge Funds Clinical Proteomics Facility and the NYU Protein Analysis Facility within the Kimmel Center for Molecular Biomedicine at the Skirball Institute at the NYU School of Medicine are dedicated to ensuring that biomedical scientists have access to cutting edge mass spectrometry technology to enable their research. We are requesting funds for a Thermo Electron LTQ-Orbitrap Velos mass spectrometer to make possible the accurate and sensitive detection, identification, characterization and relative quantitation of proteins and peptides involved in many important diseases for 11 NIH-funded major users and 5 minor users. In addition, the requested instrument would enable stable isotope labeling (SILAC) experiments for the study of intracellular signal transduction in neurons and other cells. These experiments would greatly increase our understanding of the causes and mechanisms of a number of neurodegenerative, developmental, infectious, and neuromuscular diseases and cancer.

描述（由申请人提供）：纽约大学神经科学质谱核心设施，对冲基金临床蛋白质组学设施中的100名女性和纽约大学医学院Skirball研究所Kimmel分子生物医学中心内的纽约大学蛋白质分析设施致力于确保生物医学科学家能够获得尖端的质谱技术，以使他们的研究。我们正在申请资金用于Thermo Electron LTQ-Orbitrap Velos质谱仪，以便为NIH资助的11个主要用户和5个次要用户准确和灵敏地检测，鉴定，表征和相对定量参与许多重要疾病的蛋白质和肽。此外，所要求的仪器将能够进行稳定同位素标记（SILAC）实验，用于研究神经元和其他细胞中的细胞内信号转导。这些实验将大大增加我们对许多神经退行性、发育、传染性和神经肌肉疾病和癌症的原因和机制的理解。