Protein Mass Spectrometry Core Facility for Neuroscience

描述(由申请人提供)： 自1998年成立以来，纽约大学蛋白质分析设施为纽约大学医学院的研究人员提供了尖端的基于质谱学的蛋白质分析。由于所需的昂贵和复杂的设备和专业知识，在单个实验室进行这些分析是不切实际的，甚至是不可能的。因此，对设施服务的需求迅速增加。这笔赠款的目的是提供资金，使纽约大学的神经科学家能够利用他们原本无法获得的复杂和复杂的蛋白质质谱学和蛋白质组学，并保证他们能够获得这项技术。拟议的神经科学蛋白质质谱学核心设施将使NINDS和纽约大学其他NIH资助的神经科学家能够识别从一个到数百个感兴趣的蛋白质，表征这些蛋白质的翻译后修饰，如磷酸化、糖基化、蛋白质分解和脂类修饰，进行功能蛋白质组学研究，以确定参与神经元关键信号转导过程的蛋白质，并通过表面等离子共振表征蛋白质之间的相互作用。将极大受益于这一核心设施的研究领域包括神经生长和再生、神经肌肉接头形成、癌症转移、淀粉样变性和脑出血性中风、轴突引导、轴突域组装、病变诱导的突触可塑性、钾通道功能、阿尔茨海默氏症和其他淀粉样疾病、脑发育、阿片成瘾、衰老和神经递质受体运输。

DESCRIPTION (provided by applicant): Since its establishment in 1998, the New York University Protein Analysis Facility has provided cutting edge mass spectrometry-based protein analysis to investigators at the New York University School of Medicine. Because of the expensive and sophisticated equipment and expertise required, it is impractical or impossible to perform these analyses in individual labs. As a result, demand for facility services has rapidly increased. The purpose of this grant is to provide funding to enable neuroscientists at NYU to take advantage of sophisticated and involved protein mass spectrometry and proteomics that would otherwise not be available to them, and guarantee them access to the technology. The proposed Protein Mass Spectrometry Core Facility for Neuroscience would enable NINDS- and other NIH funded neuroscientists at New York University to identify from one to many hundreds of proteins of interest, to characterize posttranslational modifications of these proteins such as phosphorylation, glycosylation, proteolysis and modification by lipids, to perform functional proteomics studies to identify the proteins involved in key signal transduction processes in neurons, and to characterize protein-protein interactions by surface plasmon resonance. Research areas that would greatly benefit from this core facility include nerve growth and regeneration, neuromuscular junction formation, cancer metastasis, amyloidosis and cerebral hemorrhagic stroke, axon guidance, axon domain assembly, lesion induced synaptic plasticity, potassium channel function, Alzheimer's and other amyloid diseases, brain development, opioid addiction, aging, and neurotransmitter receptor trafficking.