MALDI-TOF MASS SPECTROMETER

鉴定和表征引起或预防许多重要疾病的蛋白质和肽是纽约大学医学院许多NIH资助的研究项目的中心焦点。质谱分析的最新发展使人们有可能获得有关这些蛋白质的重要信息，这些信息是不切实际或不可能以任何其他方式获得的。纽约大学医学院Skirball生物分子医学研究所内的纽约大学蛋白质分析设施坚定地致力于确保这里的科学家可以从这项新技术中受益。由于其多功能性、高灵敏度、易于使用和样品处理速度快，基质辅助激光解吸飞行时间（MALDI-TOF）质谱法对我们的许多蛋白质分析至关重要。NIH资助的研究项目将受益于所要求的MALDI-TOF质谱仪的例子包括鉴定参与神经细胞发育的信号转导蛋白，用于艾滋病疫苗开发的HIV-1抗原的结构表征，鉴定参与细胞内应激反应的信号蛋白，参与胰岛素信号转导的蛋白质的结构研究，致病性金黄色葡萄球菌中外毒素产生和毒力反应的研究，调节阿片受体功能的分子机制，阿尔茨海默病中涉及的淀粉样肽的表征，志贺氏菌感染中细胞凋亡的致病作用，阿尔茨海默病和衰老的病理学，参与神经肌肉突触形成的信号蛋白，以及对B型肝炎中转录调节重要的蛋白质的研究。

The identification and characterization of proteins and peptides involved in causing or preventing many important diseases is the central focus of many NIH-funded research programs at the New York University School of Medicine. Recent developments in mass spectrometry have made it possible to gain essential information about these proteins that would be impractical or impossible to obtain in any other way. The NYU Protein Analysis Facility within the Skirball Institute of Biomolecular Medicine at-the NYU School of Medicine is strongly committed to assuring that scientists here can benefit from this new technology. Because of its versatility, high sensitivity, ease of use and high speed of sample processing, Matrix-Assisted Laser Desorption Time-of-Flight (MALDI- TOF) mass spectrometry is essential for many of our protein analyses. Examples of NIH-funded research projects that will benefit from the requested MALDI-TOF mass spectrometer include the identification of signal transduction proteins involved in nerve cell development, structural characterization of HIV-1 antigens for AIDS vaccine development, identification of signaling proteins involved in intracellular stress responses, structural studies of proteins involved in insulin signal transduction, the study of exotoxin production and virulence responses in pathogenic Staphylococcus aureus, molecular mechanisms that modulate opioid receptor function, the characterization of amyloid peptides involved in Alzheimer's Disease, the pathogenic role of apoptosis in Shigella infection, the pathology of Alzheimer's Disease and aging, signaling proteins involved in neuromuscular synapse formation, and the study of proteins that are important for transcriptional regulation in hepatitis B.