Molecular Biology/Molecular Genetics Core

分子生物学/分子遗传学（MBMG）核心的使命是协助研究人员发现肥胖及其合并症的分子基础。通过与其他NYONRC核心的合作努力，人类和啮齿动物中定义的基因改变对摄食行为、能量平衡、身体成分和内分泌功能的影响可以被表征。候选分子在组织和细胞中与能量稳态相关的作用可以通过人类受试者和啮齿动物模型的实验来描述。获得小鼠基因组的复杂操作和基因的体外操作对于产生和测试关于遗传效应传递机制的假设也至关重要。最后，利用干细胞来复制基于细胞的后果

The mission of the Molecular Biology/Molecular Genetics (MBMG) Core is to assist investigators seeking to discover the molecular bases of obesity and its co-morbidities. Through collaborative efforts with other NYONRC Cores, the effects of defined genetic alterations in humans and rodents on ingestive behavior, energy balance, body composition, and endocrine function can be characterized. The role of candidate molecules in tissues and cells related to energy homeostasis can be delineated through experimentation on both human subjects and rodent models. Access to sophisticated manipulations of the mouse genome and to in vitro manipulation of genes are also critical to generating and testing hypotheses with regard to mechanisms by which genetic effects are conveyed. Finally, the use of stem cells to replicate the cell-based consequences of genetic variation is playing a rapidly growing role in biomedical research. The modern tools of molecular biology and molecular genetic analysis comprise rapidly expanding techniques for acquiring and interpreting large amounts of data relating to gene sequence, variation in genomic architecture, gene expression and gene function. These approaches require specialized, costly equipment, skilled technical personnel and increasingly intense and complex computational analysis, all of which are inefficiently replicated in multiple individual laboratories. The objectives of the MBMG Core are to minimize barriers to the integration of cutting edge technologies of molecular genetics and genomics into the research of the Center's constituencies; to allow experienced investigators as well as researchers who are not molecular geneticists to add this dimension to their studies at reasonable cost.