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Molecular Genetics Core

分子遗传学核心

基本信息

批准号：
7418668
负责人：
RUDOLPH L LEIBEL
金额：
$ 14.62万
依托单位：
ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-04-01 至 2011-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7418668
关键词：
Adipocytes Adipose tissue Animals Appetitive Behavior Atherosclerosis Bioinformatics Biological Blast Cell Body Composition Brain Breeding Candidate Disease Gene Cloning Collaborations Comorbidity Computer software Core Facility DNA Diabetes Mellitus Endocrine Energy Metabolism Experimental Designs Facility Accesses Fatty acid glycerol esters Feeding behaviors Funding Gene Expression Gene Transfer Techniques Genes Genetic Models Genetic Techniques Genetic Variation Genome Health Services Research Homeostasis Human Human Resources Institutes Internet Islets of Langerhans Knowledge Laboratories Lead Maintenance Maps Medicine Methodology Mission Molecular Molecular Biology Molecular Genetics Mus Mutation Neurons Obesity Online Mendelian Inheritance In Man Performance Physiology PubMed Purpose QTL Genes Qualifying Quantitative Trait Loci RNA Range Reagent Research Research Personnel Rodent Rodent Model Role Services Site Students Techniques Tissues Transgenic Mice Transgenic Organisms United States National Institutes of Health Universities Work animal colony base college data acquisition design energy balance functional genomics genetic manipulation human subject investigator training mecarzole novel programs research study

项目摘要

The overarching mission of the MB/MG Core is assist investigators seeking to discover the molecular bases underlying obesity and its comorbidities. To achieve this mission, the Core performs studies in humans and rodents to identify mutations in candidate genes as well as rodent studies to define the functions of candidate genes by genetic manipulations using transgenic methodologies, the MB/MG Core makes available to biomedical investigators a wide range of methodologies and reagents relevant to understanding me molecular physiology of obesity and energy metabolism in animals and humans. This core applies molecular genetic and molecular biological techniques to the mapping, cloning and functional characterization of genes related to obesity and its comorbidities. The services of this Core are available to investigators new to obesity research, as well as to investigators working on obesity-related projects that can be enriched and extended by the use of the expertise and facilities of this core. This Core operates in 2 collaborating sites: Columbia University (R. Leibel) and AECOM (S. Chua). In general, techniques and services related to studies in humans are located at the former, and those related to rodents at tne fatter. However, this distinction is not rigid, and there is a lot of crossover between the sites. Services fndude a full range of capabilities in DNA and RNA isolation, sequencing, manipulation and quantification; experimental design and execution of gene/QTL-mapping studies in rodents and humans; maintenance of novel mouse genetic models of obesity; and design and execution of mouse transgenesis experiments.

MB/MG核心的首要使命是协助研究人员寻求发现分子基础潜在的肥胖及其合并症。为了实现这一使命，核心在人类身上进行研究，啮齿类动物，以确定候选基因的突变，以及啮齿类动物的研究，以确定功能，通过使用转基因方法进行遗传操作的候选基因，MB/MG核心使生物医学研究人员可以使用各种方法和试剂来了解我肥胖的分子生理学和动物和人类的能量代谢。核心应用利用分子遗传学和分子生物学技术，与肥胖及其合并症相关的基因的表征。本核心的服务可用于肥胖研究的新研究人员，以及从事肥胖相关项目的研究人员，通过利用这一核心的专门知识和设施来丰富和扩大。此核心在2 合作地点：哥伦比亚大学（R. Leibel）和AECOM（S. Chua）。一般来说，技术和与人类研究有关的服务位于前者，而与啮齿动物有关的服务则位于后者。然而，这种区别并不是严格的，网站之间有很多交叉。服务fndude一个完整的一系列DNA和RNA分离、测序、操作和量化的能力;实验在啮齿动物和人类中设计和执行基因/QTL作图研究;维持新的小鼠肥胖症的遗传模型;以及小鼠转基因实验的设计和执行。