Molecular Genetics Core

分子遗传学核心

• 批准号: 7418668
• 负责人: RUDOLPH L LEIBEL
• 金额: $ 14.62万
• 依托单位: ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7418668
• 关键词: Adipocytes; Adipose tissue; Animals; Appetitive Behavior; Atherosclerosis; Bioinformatics; Biological; Blast Cell; Body Composition; Brain; Breeding; Candidate Disease Gene; Cloning; Collaborations; Comorbidity; Computer software; Core Facility; DNA; Diabetes Mellitus; Endocrine; Energy Metabolism; Experimental Designs; Facility Accesses; Fatty acid glycerol esters; Feeding behaviors; Funding; Gene Expression; Gene Transfer Techniques; Genes; Genetic Models; Genetic Techniques; Genetic Variation; Genome; Health Services Research; Homeostasis; Human; Human Resources; Institutes; Internet; Islets of Langerhans; Knowledge; Laboratories; Lead; Maintenance; Maps; Medicine; Methodology; Mission; Molecular; Molecular Biology; Molecular Genetics; Mus; Mutation; Neurons; Obesity; Online Mendelian Inheritance In Man; Performance; Physiology; PubMed; Purpose; QTL Genes; Qualifying; Quantitative Trait Loci; RNA; Range; Reagent; Research; Research Personnel; Rodent; Rodent Model; Role; Services; Site; Students; Techniques; Tissues; Transgenic Mice; Transgenic Organisms; United States National Institutes of Health; Universities; Work; animal colony; base; college; data acquisition; design; energy balance; functional genomics; genetic manipulation; human subject; investigator training; mecarzole; novel; programs; research study

The overarching mission of the MB/MG Core is assist investigators seeking to discover the molecular bases underlying obesity and its comorbidities. To achieve this mission, the Core performs studies in humans and rodents to identify mutations in candidate genes as well as rodent studies to define the functions of candidate genes by genetic manipulations using transgenic methodologies, the MB/MG Core makes available to biomedical investigators a wide range of methodologies and reagents relevant to understanding me molecular physiology of obesity and energy metabolism in animals and humans. This core applies molecular genetic and molecular biological techniques to the mapping, cloning and functional characterization of genes related to obesity and its comorbidities. The services of this Core are available to investigators new to obesity research, as well as to investigators working on obesity-related projects that can be enriched and extended by the use of the expertise and facilities of this core. This Core operates in 2 collaborating sites: Columbia University (R. Leibel) and AECOM (S. Chua). In general, techniques and services related to studies in humans are located at the former, and those related to rodents at tne fatter. However, this distinction is not rigid, and there is a lot of crossover between the sites. Services fndude a full range of capabilities in DNA and RNA isolation, sequencing, manipulation and quantification; experimental design and execution of gene/QTL-mapping studies in rodents and humans; maintenance of novel mouse genetic models of obesity; and design and execution of mouse transgenesis experiments.

MB/MG Core 的首要任务是协助研究人员寻找分子基础 潜在的肥胖及其合并症。为了实现这一使命，核心对人类进行研究 啮齿类动物识别候选基因的突变以及啮齿类动物研究以确定候选基因的功能 MB/MG Core 通过使用转基因方法进行基因操作来筛选候选基因 为生物医学研究人员提供与理解相关的各种方法和试剂 动物和人类肥胖和能量代谢的分子生理学。该核心适用 分子遗传学和分子生物学技术，用于定位、克隆和功能 与肥胖及其合并症相关的基因的特征。该核心的服务可供 刚接触肥胖研究的研究人员，以及从事肥胖相关项目的研究人员 通过利用该核心的专业知识和设施来丰富和扩展。该核心运行于 2 合作地点：哥伦比亚大学 (R. Leibel) 和 AECOM (S. Chua)。一般来说，技术和 与人类研究相关的服务位于前者，与啮齿类动物相关的服务位于后者。 然而，这种区别并不严格，站点之间存在很多交叉。服务全面 DNA 和 RNA 分离、测序、操作和定量的一系列能力；实验性的 设计和执行啮齿类动物和人类的基因/QTL 作图研究；新型鼠标的维护 肥胖的遗传模型；以及小鼠转基因实验的设计和执行。