Molecular Genetics Core

MB/MG核心的首要任务是协助寻求发现分子碱基的调查人员 潜在的肥胖症及其并存。为了实现这一使命，核心进行人体研究和 啮齿动物以确定候选基因的突变，以及啮齿动物研究以确定 通过使用转基因方法进行遗传操作的候选基因，MB/MG Core制造 生物医学研究人员可获得与理解相关的各种方法和试剂 动物和人类肥胖和能量代谢的分子生理学。这一核心适用于 分子遗传学和分子生物学技术在作图、克隆和功能上的应用 肥胖症及其共病相关基因的特征。此核心的服务可用于 肥胖研究的新手，以及致力于肥胖相关项目的调查人员，这些项目可以 通过利用这一核心的专门知识和设施来丰富和推广。此核心在2个月内运行 合作网站：哥伦比亚大学(R.Leibel)和AECOM(S.Chua)。一般而言，技术和 与人类研究相关的服务位于前者，而与啮齿动物相关的服务位于较胖的位置。 然而，这种区分并不刻板，两个网站之间有很多交叉。服务提供完整的 DNA和RNA分离、测序、操作和量化能力的范围；实验性 设计和实施啮齿动物和人类的基因/QTL定位研究；保持新的小鼠 肥胖的遗传模型；以及小鼠转基因实验的设计和执行。

The overarching mission of the MB/MG Core is assist investigators seeking to discover the molecular bases underlying obesity and its comorbidities. To achieve this mission, the Core performs studies in humans and rodents to identify mutations in candidate genes as well as rodent studies to define the functions of candidate genes by genetic manipulations using transgenic methodologies, the MB/MG Core makes available to biomedical investigators a wide range of methodologies and reagents relevant to understanding me molecular physiology of obesity and energy metabolism in animals and humans. This core applies molecular genetic and molecular biological techniques to the mapping, cloning and functional characterization of genes related to obesity and its comorbidities. The services of this Core are available to investigators new to obesity research, as well as to investigators working on obesity-related projects that can be enriched and extended by the use of the expertise and facilities of this core. This Core operates in 2 collaborating sites: Columbia University (R. Leibel) and AECOM (S. Chua). In general, techniques and services related to studies in humans are located at the former, and those related to rodents at tne fatter. However, this distinction is not rigid, and there is a lot of crossover between the sites. Services fndude a full range of capabilities in DNA and RNA isolation, sequencing, manipulation and quantification; experimental design and execution of gene/QTL-mapping studies in rodents and humans; maintenance of novel mouse genetic models of obesity; and design and execution of mouse transgenesis experiments.