Molecular Biology/Molecular Genetics Core

分子生物学/分子遗传学核心

• 批准号: 7120332
• 负责人: RUDOLPH L LEIBEL
• 金额: $ 12.61万
• 依托单位: ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7120332
• 关键词: Rodentias; adipocytes; bioenergetics; biomedical facility; body composition; disease /disorder model; endocrinology; functional /structural genomics; gene expression; genetic mapping; genetic models; genetically modified animals; human subject; metabolism; microarray technology; model design /development; molecular biology; molecular genetics; molecular pathology; neurons; nutrient intake activity; nutrition related tag; obesity; pancreatic islets; protein structure function

The overarching mission of the MB/MG Core is assist investigators seeking to discover the molecular bases underlying obesity and its comorbidities. To achieve this mission, the Core performs studies in humans and rodents to identify mutations in candidate genes as well as rodent studies to define the functions of candidate genes by genetic manipulations using transgenic methodologies, the MB/MG Core makes available to biomedical investigators a wide range of methodologies and reagents relevant to understanding me molecular physiology of obesity and energy metabolism in animals and humans. This core applies molecular genetic and molecular biological techniques to the mapping, cloning and functional characterization of genes related to obesity and its comorbidities. The services of this Core are available to investigators new to obesity research, as well as to investigators working on obesity-related projects that can be enriched and extended by the use of the expertise and facilities of this core. This Core operates in 2 collaborating sites: Columbia University (R. Leibel) and AECOM (S. Chua). In general, techniques and services related to studies in humans are located at the former, and those related to rodents at tne fatter. However, this distinction is not rigid, and there is a lot of crossover between the sites. Services fndude a full range of capabilities in DNA and RNA isolation, sequencing, manipulation and quantification; experimental design and execution of gene/QTL-mapping studies in rodents and humans; maintenance of novel mouse genetic models of obesity; and design and execution of mouse transgenesis experiments.

MB/mg核心的总体任务是协助寻求发现分子基础的研究人员 潜在的肥胖及其合并症。为了实现这一任务，核心在人类和 啮齿动物以鉴定候选基因中的突变以及啮齿动物研究以定义的功能 通过使用转基因方法学的遗传操纵候选基因，MB/mg核心使得 生物医学研究人员可以使用与理解有关的广泛方法和试剂 我的肥胖和能量代谢分子生理学中的动物和人类。此核心适用 分子遗传和分子生​​物学技术，用于映射，克隆和功能 与肥胖及其合并症有关的基因的表征。该核心的服务可用于 肥胖研究的新研究人员以及从事与肥胖相关项目的研究人员 通过使用该核心的专业知识和设施来丰富和扩展。该核心在2中运行 合作站点：哥伦比亚大学（R. Leibel）和AECOM（S。Chua）。通常，技术和 与人类研究有关的服务位于前者，与啮齿动物有关的服务与啮齿动物有关。 但是，这种区别并不僵硬，并且这些站点之间有很多交叉。服务fndude DNA和RNA分离，测序，操纵和定量的功能范围；实验 在啮齿动物和人类中的基因/QTL映射研究的设计和执行；维持新老鼠 肥胖的遗传模型；小鼠转基因实验的设计和执行。