Molecular Biology/Molecular Genetics Core

分子生物学/分子遗传学核心

• 批准号: 7120332
• 负责人: RUDOLPH L LEIBEL
• 金额: $ 12.61万
• 依托单位: ST. LUKE'S-ROOSEVELT INST FOR HLTH SCIS
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7120332
• 关键词: Rodentias; adipocytes; bioenergetics; biomedical facility; body composition; disease /disorder model; endocrinology; functional /structural genomics; gene expression; genetic mapping; genetic models; genetically modified animals; human subject; metabolism; microarray technology; model design /development; molecular biology; molecular genetics; molecular pathology; neurons; nutrient intake activity; nutrition related tag; obesity; pancreatic islets; protein structure function

The overarching mission of the MB/MG Core is assist investigators seeking to discover the molecular bases underlying obesity and its comorbidities. To achieve this mission, the Core performs studies in humans and rodents to identify mutations in candidate genes as well as rodent studies to define the functions of candidate genes by genetic manipulations using transgenic methodologies, the MB/MG Core makes available to biomedical investigators a wide range of methodologies and reagents relevant to understanding me molecular physiology of obesity and energy metabolism in animals and humans. This core applies molecular genetic and molecular biological techniques to the mapping, cloning and functional characterization of genes related to obesity and its comorbidities. The services of this Core are available to investigators new to obesity research, as well as to investigators working on obesity-related projects that can be enriched and extended by the use of the expertise and facilities of this core. This Core operates in 2 collaborating sites: Columbia University (R. Leibel) and AECOM (S. Chua). In general, techniques and services related to studies in humans are located at the former, and those related to rodents at tne fatter. However, this distinction is not rigid, and there is a lot of crossover between the sites. Services fndude a full range of capabilities in DNA and RNA isolation, sequencing, manipulation and quantification; experimental design and execution of gene/QTL-mapping studies in rodents and humans; maintenance of novel mouse genetic models of obesity; and design and execution of mouse transgenesis experiments.

MB/MG核心的首要使命是协助研究人员寻求发现分子基础 潜在的肥胖及其合并症。为了实现这一使命，核心在人类身上进行研究， 啮齿类动物，以确定候选基因的突变，以及啮齿类动物的研究，以确定功能， 通过使用转基因方法进行遗传操作的候选基因，MB/MG核心使 生物医学研究人员可以使用各种方法和试剂来了解 我肥胖的分子生理学和动物和人类的能量代谢。核心应用 利用分子遗传学和分子生物学技术， 与肥胖及其合并症相关的基因的表征。本核心的服务可用于 肥胖研究的新研究人员，以及从事肥胖相关项目的研究人员， 通过利用这一核心的专门知识和设施来丰富和扩大。此核心在2 合作地点：哥伦比亚大学（R. Leibel）和AECOM（S. Chua）。一般来说，技术和 与人类研究有关的服务位于前者，而与啮齿动物有关的服务则位于后者。 然而，这种区别并不是严格的，网站之间有很多交叉。服务fndude一个完整的 在DNA和RNA分离、测序、操作和定量方面的能力范围;实验 在啮齿动物和人类中设计和执行基因/QTL作图研究;维持新的小鼠 肥胖症的遗传模型;以及小鼠转基因实验的设计和执行。