Repair of tendinopathic tendons

肌腱病变肌腱的修复

• 批准号: 8479211
• 负责人: JAMES H-C. WANG
• 金额: $ 32.38万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8479211
• 关键词: Address; Adipocytes; Adopted; Affect; Age; American; Animal Model; Athletic; Blood Platelets; Calcified; Cell Lineage; Cell physiology; Cells; Cellular Structures; Clinical Treatment; Clinical Trials; Collagen Type I; Combined Modality Therapy; Control Animal; Data; Deposition; Dinoprostone; Disease; Engineering; Enzymes; Evaluation; Gender; Goals; Growth Factor; Healed; High Prevalence; Histocytochemistry; Histologic; Human; Immunohistochemistry; In Situ; In Vitro; Inflammation; Inflammation Mediators; Injection of therapeutic agent; Interstitial Collagenase; Knowledge; Lead; Lesion; Light; Lipids; Measures; Mechanics; Modeling; Mus; Natural regeneration; Occupational; Orthopedics; Oryctolagus cuniculus; Osteocytes; Pain; Patients; Performance at work; Phenotype; Physicians; Plasma; Play; Population; Prevalence; Property; Proteins; Proteoglycan; Quality of life; Recording of previous events; Recovery; Safety; Sampling; Smooth Muscle Actin Staining Method; Sports Medicine; Staging; Stem cells; Stromelysin 1; Structure; Tendinopathy; Tendon structure; Testing; Time; Tissue Inhibitor of Metalloproteinase-1; Tissues; United States; Use Effectiveness; Western Blotting; Work; base; cellular engineering; clinically relevant; collagenase 3; design; healing; human subject; implantation; improved; in vivo; inhibitor/antagonist; injured; innovation; regenerative; repaired; self-renewal; stem cell differentiation; translational study; treatment effect

DESCRIPTION (provided by applicant): Tendinopathy, or degenerative tendons, affects millions of Americans in both occupational and athletic settings each year. Tendinopathy causes pain and discomfort, impairs work performance, and reduces quality of life. In spite of its high prevalence, current treatments of tendinopathy are not effective in restoring normal structure and function to degenerated tendons. In recent years, platelet-rich plasma (PRP) has been a popular treatment option for tendinopathy; however, whether PRP treatment is effective remains a huge controversy in orthopaedic/sports medicine. The overall objective of this project is to address this issue by defining the effects of clinically-relevant PRP treatments, along with tendon stem cells (TSCs) and engineered tendon matrix (ETM), on a validated rabbit tendinopathy model at the cellular, tissue, and functional levels. The specific aims of this project are: 1) to evaluate the effects of PRP treatment on the repair of tendinopathic tendons by PRP injection; 2) to evaluate the effects of PRP treatment on the repair of tendinopathic tendons by implantation of PRP with TSCs; and 3) to evaluate the effects of adding ETM on the repair of tendinopathic tendons. This study is highly significant in light of the fact that PRP treatment is widely used in orthopaedic/sports medicine for tendinopathy treatment in the United States despite little available scientific data that support or refute its usages. This study is also highly innovative since a potent combination of natural healing growth factors (PRP), tendon-specific regenerative cells (TSCs), and tendon specific matrix (ETM) will be explored for the first time to enhance the repair of tendinopathic tendons. We anticipate that the findings of this study will lead to the application of this combination therapy clinically to effectively repair or possibly regenerate tendinopathic tendons, thus benefiting millions of tendinopathic patients in the United States alone.

描述（由申请人提供）：肌腱病或退行性肌腱每年影响数百万美国人的职业和运动环境。肌腱病会导致疼痛和不适，影响工作表现并降低生活质量。尽管其患病率很高，但目前的肌腱病治疗方法并不能有效恢复退化肌腱的正常结构和功能。近年来，富血小板血浆（PRP）已成为肌腱病的流行治疗选择。然而，PRP治疗是否有效在骨科/运动医学界仍存在巨大争议。该项目的总体目标是通过定义临床相关 PRP 治疗以及肌腱干细胞 (TSC) 和工程肌腱基质 (ETM) 在细胞、组织和功能水平上对经过验证的兔肌腱病模型的影响来解决这个问题。该项目的具体目的是：1）评估PRP治疗对注射PRP修复肌腱病变的效果； 2）通过植入PRP和TSC来评估PRP治疗对肌腱病变肌腱修复的效果； 3)评价添加ETM对肌腱病变肌腱修复的效果。鉴于 PRP 治疗在美国的骨科/运动医学中广泛用于肌腱病治疗，尽管几乎没有可用的科学数据支持或反驳其用途，因此这项研究具有非常重要的意义。这项研究也具有高度创新性，因为将首次探索自然愈合生长因子（PRP）、肌腱特异性再生细胞（TSC）和肌腱特异性基质（ETM）的有效组合，以增强肌腱病变肌腱的修复。我们预计这项研究的结果将导致这种联合疗法在临床上的应用，以有效修复或可能再生肌腱病肌腱，从而使仅在美国数百万肌腱病患者受益。