Structures and Activities of Prions and Prion Proteins

2013财年：1)我们致力于通过(在我们的实验室)应用新的生物化学、免疫学、光谱、电子显微镜和计算方法来定义感染性Prion的结构，从而更好地了解Prion的结构。我们已经开发了新的Pron结构模型，正在根据我们自己的新数据以及已发表的报告进行构建和评估。这些模型基于并行寄存器测试表体系结构，其他人已经提出了该体系结构的部分版本。这些进展正在进行中，预计很快就会有重大发现。2)我们已经评估了正常的Prion蛋白在Toll样受体配体的刺激物反应中的作用，没有发现任何显著的影响。3)探讨了正常蛋白在C57/BL系小鼠实验性自身免疫性脑脊髓炎发病中的作用，未发现明显作用。

In FY2013: 1) We have worked toward a better understanding of prion structure by initiating the application of new (to our lab) biochemical, immunological, spectroscopic, electron microscopic, and computational approaches to defining the structure of infectious prions. We have developed new models of prion structure is being built and evaluated based on our own new data, as well as published reports. These models are based on parallel-in-register beta sheet architectures, partial versions of which have been proposed by others. These developments are in progress and are expected to lead to significant discoveries soon. 2) We have assessed the role of normal prion protein in responses to stimulators of toll-like receptor ligands and have not found any significant effects. 3) We have probed the role of normal prion protein in the experimental autoimmune encephalomyelitis in mice of the C57/BL background and found no significant effect.