Detection of Prions

朊病毒蛋白的病理亚型（PrPres）可以作为朊病毒疾病的标志物，但需要更多的实际测试来进行临床前诊断和敏感检测许多朊病毒感染。在2011财年，我们1)开发了一种新的朊病毒检测方法（称为eQuIC），允许在含有抑制剂的生物液体（如血浆）中超灵敏和快速地检测朊病毒；2)使eQuIC适用于人、羊、仓鼠和小鼠血浆样品中朊病毒的检测；3)培训并与人类TSE监测组合作，评估我们之前开发的RT-QuIC检测在诊断人类散发克雅氏病中的应用；数据显示，RT-QuIC应用于临床脑脊液样本时，比以往任何诊断测试都具有更好的敏感性和特异性。

The pathological isoform of the prion protein (PrPres) can serve as a marker for prion diseases, but more practical tests are needed for preclinical diagnosis and sensitive detection of many prion infections. During FY2011, we 1) developed a new prion assay (called eQuIC) that allows for the ultrasensitive and rapid of prions in inhibitor-laden biological fluids such as blood plasma; 2) adapted eQuIC to the detection of human, sheep, hamster and mouse prions in blood plasma samples; 3) trained and collaborated with human TSE surveillance groups to evaluate the utility of our previously developed RT-QuIC assay for the diagnosis of human sporadic Creutzfeldt-Jakob disease; the data showed that when applied to clinical cerebral spinal fluid samples, RT-QuIC has better sensitivity and specificity than any prior diagnostic test.