Detection of Prions

朊病毒检测

• 批准号: 8336296
• 负责人: BYRON CAUGHEY
• 金额: $ 97.61万
• 依托单位: NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8336296
• 关键词: Agriculture; Biological; Biological Assay; Bovine Spongiform Encephalopathy; Cerebrospinal Fluid; Chronic Wasting Disease; Clinic; Clinical; Creutzfeldt-Jakob Syndrome; Data; Detection; Diagnosis; Diagnostic tests; Disease; Hamsters; Human; Infection; Liquid substance; Mus; Neurodegenerative Disorders; Plasma; Prion Diseases; Prions; Protein Isoforms; Sampling; Scrapie; Sensitivity and Specificity; Sheep; Testing; Training; base; inhibitor/antagonist; polymerization; pre-clinical; prion seeds

The pathological isoform of the prion protein (PrPres) can serve as a marker for prion diseases, but more practical tests are needed for preclinical diagnosis and sensitive detection of many prion infections. During FY2011, we 1) developed a new prion assay (called eQuIC) that allows for the ultrasensitive and rapid of prions in inhibitor-laden biological fluids such as blood plasma; 2) adapted eQuIC to the detection of human, sheep, hamster and mouse prions in blood plasma samples; 3) trained and collaborated with human TSE surveillance groups to evaluate the utility of our previously developed RT-QuIC assay for the diagnosis of human sporadic Creutzfeldt-Jakob disease; the data showed that when applied to clinical cerebral spinal fluid samples, RT-QuIC has better sensitivity and specificity than any prior diagnostic test.

Prion蛋白的病理异构体(PrPres)可以作为Prion疾病的标志物，但需要更多的实用测试来进行临床前诊断和对许多Prion感染的敏感检测。2011财年期间，我们1)开发了一种新的Prion分析方法(称为eQuIC)，它能够对血浆等富含抑制剂的生物液中的Prion进行超灵敏和快速的检测；2)使eQuIC适用于检测血浆样本中的人、绵羊、仓鼠和小鼠Prion；3)对人类TSE监督小组进行了培训并与其合作，评估了我们先前开发的RT-QuIC方法用于诊断散发性人类CreutzFeldt-Jakob病的有效性；数据表明，当应用于临床脑脊液样本时，RT-QuIC比以前的任何诊断测试都具有更高的灵敏度和特异性。