A Mechanical Nanomembrane Detector for Time-of-Flight Mass Spectrometry

用于飞行时间质谱分析的机械纳米膜探测器

基本信息

  • 批准号:
    8435393
  • 负责人:
  • 金额:
    $ 29.05万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2012
  • 资助国家:
    美国
  • 起止时间:
    2012-03-01 至 2015-02-28
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (provided by applicant): Existing detectors for time-of-flight (TOF) mass spectrometry of intact proteins in low charge states, such as microchannel plates and electron multipliers, rely upon the emission of secondary electrons for ion detection. Unfortunately, the efficiency of this secondary electron generation falls-off severely with increasing mass of the incident ions, dramatically reducing detection sensitivity and limiting the ability of TOF mass spectrometers to provide useful mass information on large biomolecules. This problem in ion detection is one of the major reasons that biological mass spectrometry as currently practiced is predominantly directed towards the analysis of small peptides rather than towards whole intact proteins, a critical limitation in the technology. We have developed a new type of ion detector to address this problem, based upon the mechanical deformation and vibration of a nanomembrane. An incoming ion packet initiates oscillations of the nanomembrane, which are then detected by corresponding oscillations in field emission electron current from the membrane. We propose here to develop our initial prototype detector into a powerful, robust, and well- characterized device for the mass spectrometry of intact proteins up to a megadalton in size. This new detector technology will open many new opportunities in biological mass spectrometry, in areas such as biomarker discovery and monitoring, the elucidation of protein variation, and the imaging of tissue by MALDI mass spectrometry.
描述(由申请人提供):用于低电荷状态下完整蛋白质的飞行时间(TOF)质谱分析的现有检测器,如微通道板和电子倍增器,依赖于发射二次电子进行离子检测。不幸的是,这种二次电子产生的效率随着入射离子质量的增加而严重下降,显著降低了检测灵敏度并限制了TOF质谱仪提供关于大生物分子的有用质量信息的能力。离子检测中的这个问题是目前实践的生物质谱法主要针对小肽而不是针对完整蛋白质的分析的主要原因之一,这是该技术的关键限制。 我们开发了一种新型离子检测器, 基于纳米膜的机械变形和振动来解决这个问题。进入的离子包引发纳米膜的振荡,然后通过来自膜的场发射电子电流中的相应振荡来检测振荡。我们建议在这里开发我们的初始原型检测器成为一个强大的,强大的,以及表征完整的蛋白质的质谱仪高达兆道尔顿的大小的设备。 这种新的检测器技术将在生物质谱中开辟许多新的机会,例如生物标志物的发现和监测,蛋白质变异的阐明以及MALDI质谱的组织成像。

项目成果

期刊论文数量(0)
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LLOYD M SMITH其他文献

LLOYD M SMITH的其他文献

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{{ truncateString('LLOYD M SMITH', 18)}}的其他基金

Dehydroamino acids in HIV-1 capsid and matrix proteins: new potential targets for viral inactivation
HIV-1衣壳和基质蛋白中的脱氢氨基酸:病毒灭活的新潜在靶点
  • 批准号:
    10762067
  • 财政年份:
    2023
  • 资助金额:
    $ 29.05万
  • 项目类别:
Novel NeuCode Tagging Reagents for Identification and Quantification of Intact Proteoforms in Cancer Tissues
用于癌症组织中完整蛋白质形式的识别和定量的新型 NeuCode 标记试剂
  • 批准号:
    9443408
  • 财政年份:
    2018
  • 资助金额:
    $ 29.05万
  • 项目类别:
Novel Technologies for Protein Analysis
蛋白质分析新技术
  • 批准号:
    9912172
  • 财政年份:
    2018
  • 资助金额:
    $ 29.05万
  • 项目类别:
Novel Technologies for Protein Analysis
蛋白质分析新技术
  • 批准号:
    10226834
  • 财政年份:
    2018
  • 资助金额:
    $ 29.05万
  • 项目类别:
Revealing Proteoforms: The Primary Effectors of Biological Function
揭示蛋白质形式:生物功能的主要效应器
  • 批准号:
    10621058
  • 财政年份:
    2018
  • 资助金额:
    $ 29.05万
  • 项目类别:
Novel Technologies for Protein Analysis
蛋白质分析新技术
  • 批准号:
    10378644
  • 财政年份:
    2018
  • 资助金额:
    $ 29.05万
  • 项目类别:
Development and Applications of High Density RNA Arrays
高密度RNA阵列的开发及应用
  • 批准号:
    9130258
  • 财政年份:
    2014
  • 资助金额:
    $ 29.05万
  • 项目类别:
Proteomics Core
蛋白质组学核心
  • 批准号:
    8381279
  • 财政年份:
    2012
  • 资助金额:
    $ 29.05万
  • 项目类别:
A Mechanical Nanomembrane Detector for Time-of-Flight Mass Spectrometry
用于飞行时间质谱分析的机械纳米膜探测器
  • 批准号:
    8273631
  • 财政年份:
    2012
  • 资助金额:
    $ 29.05万
  • 项目类别:
QPASS: Quantitative Parallel Aptamer Selection System
QPASS:定量平行适体选择系统
  • 批准号:
    8335434
  • 财政年份:
    2011
  • 资助金额:
    $ 29.05万
  • 项目类别:

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