Development and Application of New Ionization Methods for Biological Mass Spectro

生物质谱新型电离方法的开发与应用

基本信息

批准号：
8516528
负责人：
Hooman Troy Ghashghaei
金额：
$ 29.21万
依托单位：
NORTH CAROLINA STATE UNIVERSITY RALEIGH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-09-01 至 2015-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8516528
关键词：
Address Amines Atmospheric Pressure Biological Biological Markers Carbohydrates Characteristics Charge Chemicals Classification Data Development Diagnostic Disease Dissociation Electrospray Ionization Ensure Foundations Fresh Tissue Health Heart Diseases Human Hybrids Hydrophobicity Image Image Analysis Immune System Diseases Ions Knowledge Lasers Lipids Liquid substance Literature Malignant Neoplasms Mass Spectrum Analysis Measurement Measures Metals Methodology Methods Molecular Molecular Models Molecular Profiling Mus Nucleic Acids Peptides Play Preparation Prognostic Marker Proteins Proteomics Reporting Research Role Sampling Science Solid Solutions Solvents Source Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Stable Isotope Labeling Surface System Systems Biology Techniques Technology Therapeutic Tissues Translating analytical tool base clinically relevant design instrumentation ionization ionization technique mass analyzer mass spectrometer metabolomics molecular imaging molecular modeling neurodevelopment public health relevance research and development solid state

项目摘要

DESCRIPTION (provided by applicant): Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there are still opportunities for new advances to be made allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to allow for tissue imaging with top-down characterization. The short-term objective of this proposal is to develop and understand these new ionization methods using chemical and molecular systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging. The long- term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the "weighing of molecules", has had a profound impact on the study of human health and disease including cancer, heart disease, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be "weighed" by the mass spectrometer. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide diagnostic and prognostic biomarkers.

描述（由申请人提供）：质谱是一种非常强大的生物分析技术，对我们对人类健康和疾病的分子理解产生了深远的影响。质量分析仪技术，解离技术和电离方法的重大进展主要归因于质谱在系统生物学领域中扮演的核心作用。尽管在上个世纪中，质谱已演变为一种高效的分析工具，但仍有机会可以使新的进步允许解决各种各样的生物学问题。该建议集中在生物学质谱新的电离方法的开发上，以允许具有自上而下表征的组织成像。该提案的短期目标是使用化学和分子系统开发和理解这些新的电离方法。这些结果将为生物应用将直接受益的坚实基础。在这种心态中，我们将开发并将这些新的电离方法应用于组织成像。长期目标是建立这些新的电离方法作为一种使生物分析技术有效解决人类健康和疾病问题的一种。对拟议研究质谱法（MS）的公众描述，与“称重分子”有关的科学对人类健康和疾病的研究产生了深远的影响，包括癌症，心脏病，神经发育和自身免疫性疾病。 MS的先决条件是将中性分子转换为带电物种（离子），以便可以通过质谱仪“称重”它们。这项研究的重点是开发新的电离方法，允许解决各种各样的当代生物医学问题。这将包括组织成像，以最终提供诊断和预后的生物标志物。