Development and Application of New Ionization Methods for Biological Mass Spectro

生物质谱新型电离方法的开发与应用

• 批准号: 7981584
• 负责人: Hooman Troy Ghashghaei
• 金额: $ 27.3万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7981584
• 关键词: Address; Amines; Atmospheric Pressure; Biological; Biological Markers; Carbohydrates; Characteristics; Charge; Chemicals; Classification; Data; Development; Diagnostic; Disease; Dissociation; Electrospray Ionization; Ensure; Foundations; Fresh Tissue; Health; Heart Diseases; Human; Hybrids; Hydrophobicity; Image; Image Analysis; Immune System Diseases; Ions; Knowledge; Lasers; Lipids; Liquid substance; Literature; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Measures; Metals; Methodology; Methods; Molecular; Molecular Models; Molecular Profiling; Mus; Nucleic Acids; Peptides; Play; Preparation; Proteins; Proteomics; Reporting; Research; Role; Sampling; Science; Solid; Solutions; Solvents; Source; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stable Isotope Labeling; Surface; System; Systems Biology; Techniques; Technology; Therapeutic; Tissues; Translating; analytical tool; base; clinically relevant; design; instrumentation; ionization; ionization technique; mass analyzer; mass spectrometer; metabolomics; molecular modeling; neurodevelopment; prognostic; public health relevance; research and development; solid state

DESCRIPTION (provided by applicant): Mass spectrometry is an extraordinarily powerful bioanalytical technique that has had a profound impact on our molecular understanding of human health and disease. Major advances in mass analyzer technology, dissociation techniques, and ionization methods are largely attributed to the central role that mass spectrometry plays in the field of systems biology. While mass spectrometry has evolved over the last century into a highly effective analytical tool, there are still opportunities for new advances to be made allowing an even more diverse array of biological questions to be addressed. This proposal is centered on the development of new ionization methods for biological mass spectrometry to allow for tissue imaging with top-down characterization. The short-term objective of this proposal is to develop and understand these new ionization methods using chemical and molecular systems. These results will provide a solid foundation from which biological applications will directly benefit. In this mindset, we will develop and apply these new ionization methods to tissue imaging. The long- term objective is to establish these new ionization methods as an enabling bioanalytical technology to effectively address questions in human health and disease. Public Description of Proposed Research Mass spectrometry (MS), the science related to the "weighing of molecules", has had a profound impact on the study of human health and disease including cancer, heart disease, neural development, and auto-immune diseases. A prerequisite of MS is to convert neutral molecules into charged species (ions) such that they can be "weighed" by the mass spectrometer. The focus of this research is to develop new ionization methods allowing a more diverse array of contemporary biomedical questions to be addressed. This will include the imaging of tissues to ultimately provide diagnostic and prognostic biomarkers. PUBLIC HEALTH RELEVANCE: This proposal seeks support to develop new ionization methods for biological mass spectrometry that will enable tissue imaging with identification via top-down methods. Furthermore, these ionization methods will be broadly applicable to the fields of proteomics and systems biology. In summary, we will establish a new enabling bioanalytical technology through detailed characterization using model molecular and chemical systems and demonstrate its application to tissue imaging.

描述（由申请人提供）：质谱是一种非常强大的生物分析技术，对我们对人类健康和疾病的分子理解产生了深远的影响。质量分析仪技术，解离技术和电离方法的重大进展主要归因于质谱在系统生物学领域中扮演的核心作用。尽管在上个世纪中，质谱已演变为一种高效的分析工具，但仍有机会可以使新的进步允许解决各种各样的生物学问题。该建议集中在生物学质谱新的电离方法的开发上，以允许具有自上而下表征的组织成像。该提案的短期目标是使用化学和分子系统开发和理解这些新的电离方法。这些结果将为生物应用将直接受益的坚实基础。在这种心态中，我们将开发并将这些新的电离方法应用于组织成像。长期目标是建立这些新的电离方法作为一种使生物分析技术有效解决人类健康和疾病问题的一种。对拟议研究质谱法（MS）的公众描述，与“称重分子”有关的科学对人类健康和疾病的研究产生了深远的影响，包括癌症，心脏病，神经发育和自身免疫性疾病。 MS的先决条件是将中性分子转换为带电物种（离子），以便可以通过质谱仪“称重”它们。这项研究的重点是开发新的电离方法，允许解决各种各样的当代生物医学问题。这将包括组织成像，以最终提供诊断和预后的生物标志物。 公共卫生相关性：该提案寻求支持，以开发新的电离方法，用于生物学质谱法，该方法将通过自上而下的方法通过识别来实现组织成像。此外，这些电离方法将广泛适用于蛋白质组学和系统生物学领域。总而言之，我们将通过使用模型分子和化学系统的详细表征来建立一种新的启用生物分析技术，并证明其在组织成像中的应用。