A Functionalized Nanoparticle-Based handheld Device for Rapid and Sensitive Detec

描述：有机磷(OP)杀虫剂是一种剧毒化合物，在许多农业和园林应用中用于控制昆虫种群。这些有毒化学品的广泛使用已经产生了严重的环境健康风险。对有机磷农药环境暴露的敏感和准确的生物监测及其健康风险评估是至关重要的。然而，用于检测环境有机磷暴露的简单、快速和定量的诊断技术和设备是不可用的。该小型企业创新研究项目旨在开发一种手持式生物监测设备，该设备结合了功能化纳米颗粒和侧向流动测试试纸，用于使用血液样本简单、快速、经济有效地定量检测环境中OP的暴露。该项目利用磷酸化胆碱酯酶(OP-CHE)作为OP暴露的生物标志物。在第一阶段，有三个具体的目标：(1)开发与OP-ChE结合的Zr(IV)功能化荧光纳米颗粒(Zr-FFNPs)。(2)建立了一种新的检测OP-ChE的快速、灵敏的免疫分析方法。(3)将基于Zr-FFNP的免疫分析方法应用于检测OP-ChE的侧向流动试纸系统。这项研究将使用三种典型农药的体外血液样本来确定该设备的检测下限、响应时间、动态范围等关键性能指标，并证明手持生物监测设备用于灵敏检测OP暴露的可行性。第二阶段将进一步开发一种专门的手持式生物监测设备，用于检测接触OP的情况。该装置将通过体外注入各种有机磷农药的血液以及农场附近学校的学生和参与使用有机磷杀虫剂的工人家属的血液样本进行验证。根据该计划开发的便携式生物监测设备将为快速、灵敏、经济有效和实时地检测环境中有机磷农药的暴露提供一种护理点工具。

DESCRIPTION: Organophosphate (OP) pesticides are highly toxic compounds used to control insect populations in a number of agricultural and landscaping applications. The widespread use of these toxic chemicals has generated serious environmental health risks. It is vital to sensitively and accurately bio monitoring of environmental exposure to OP pesticides and assessment of its health risk. However, simple, rapid, and quantitative diagnostic technologies and devices for detecting environmental OP exposure are not available. This Small Business Innovation Research Project is to develop a handheld bio monitoring device incorporating a functionalized nanoparticle and a lateral flow test strip for simple, rapid, cost-effective, and quantitative detection of environmental exposure to OP using a blood sample. This project takes advantage of phosphorylated cholinesterase (OP-ChE) as a biomarker of OP exposure. In phase I, there are three specific aims: (1) Develop Zr (IV)-functionalized fluorescence nanoparticles (Zr-FFNPs) that bind to OP-ChE. (2) Develop a Zr-FFNP-based new immunoassay for detecting OP-ChE. (3) Adapt the Zr-FFNP-based immunoassay to a lateral flow test strip system for detecting OP-ChE. The research will determine the detection limits, response time, dynamic range, and other key performance metrics of the device using blood samples in vitro dosed with three typical pesticides and prove the feasibility of the handheld bio monitoring device for sensitively detecting OP exposure. Phase II will further develop a specialized hand-held bio monitoring device for detecting exposure to OP. The device will be validated with blood in vitro dosed with a wide range of OP pesticides and blood samples from students in schools near farms and family members of workers who involved in the used of OP pesticides. The portable biomonitoring device developed under this program will provide a point-of-care tool for rapid, sensitive, cost-effective, and real-time detection of environmental exposure to OP pesticides.