Research Services

研究服务核心为所有三个项目和该计划中的其他两个核心提供基本支持。麻省理工学院生理系的核心设施在过去25年里从最初的单一计划项目拨款的核心设施发展而来。随着国防部的发展，调查人员发现，这些设施对他们的研究越来越重要。目前的核心设施部分由该部和其他研究补助金提供支助。PPG对这些核心设施的持续财政支持是 对本计划中提议的工作至关重要。没有这些核心，本申请中提出的研究无法进行。 该计划的研究服务核心在前一次资助期间扩大了范围和规模，增加了组织学、显微镜和图像处理方面的新活动。目前有11名研究人员在生理学系内支持研究基础设施的广泛活动中拥有丰富的经验。这名工作人员被分配到一个或多个支持小组，计算机、分析、动物监测、显微镜、机械和电子设备以及仪器 服务。根据这些小组活动的交叉数量，我们现在发现，在安德鲁斯博士的监督下，将他们的努力统一到一个核心中会更有成效。格林。在这些自然划分的基础上，研究服务核心在计划项目中提供六项主要职能：计算机支持、分析和统计咨询、慢性动物监测、显微镜和图像处理、定制机器和电子制造以及仪器服务。这些功能中的每一项都由配备齐全的设施提供服务，该设施随时可供程序调查人员使用。 系统管理是维持大型和复杂的研究工作的一个关键因素，例如由研究人员/研究中心工作人员支持的工作。这一概念适用于研究事务核心的所有领域，以最大限度地提高主要调查人员使用的研究设备和设施的设计和维护的效率和灵活性。将研究方案的不同组成部分集成到可重复和可管理的系统中的能力是将具有互补技能集的有经验的个人组合到给定设计问题的结果。其目标是为从实验室到出版的研究计划提供技术基础设施。提供标准的数据采集系统、分析程序、数据管理和出版工具，同时扩展和维护这些系统，以满足研究目标，是这一核心的主要活动。 研究服务核心继续向方案项目赠款提供直接支助，提供计算机硬件、软件和网络，并有机会接触到有能力设计和维修专门研究设备的熟练机械师和电子工程师l一个行政和监督单位提供的设施和技能使重要实验室设备的维护和维修费用大大降低。集成设备管理的所有方面，通过高效储存设备加快维修时间 关键备件和有效利用外部专门维修服务和合同。为设备采购和服务提供单点联系，使计划项目调查人员能够从采购整合中受益，同时加强供应商沟通和评估。它还为共享设备的维修手册和用户文档提供托管存储库。维修历史和维护记录为设备生命周期管理决策提供成本效益输入。 计算机服务和分析组件历来是本方案项目的重要组成部分，一直为所有项目和核心提供服务。这一核心的增长是因为它处于该计划综合活动的中心，因为它确实是三个项目之间的关键纽带，而基因组学核心B是该计划中至关重要且使用最频繁的资源。 在之前的资助期间，我们通过与MCW的生物技术和生物医学工程中心发展密切合作，扩大了我们进行理论工作的能力。这种关系促进了中心和生理系的相互需求，取得了极大的成功。在这一模式下，在HMGC内的生物信息学研究中心以及生物统计司的密切合作下，PPG调查人员可以获得大规模、最先进的计算以及复杂数据分析和建模方面的专业知识。 PPG的机器和电子核心使计划项目的研究人员能够接触到熟练的机械师和电子工程师，他们能够设计和维修专门的研究设备L在先前的提案中，这些活动被并入研究服务核心，以便在一个行政和监督单位下提供计划的所有研究支持活动。PPG调查人员大量使用机器和电子车间来设计和制造以及维修VITAL 他们实验室里的设备。由于我们的计划中有熟练的机械师和工程师，我们已经能够减少对外部服务组织的依赖。

The Research Services Core provides basic support for all three Projects and the other two Cores in this Program. The Core facilities in the Department of Physiology at MCW evolved over the last twenty five years from their origin as a Core from a single Program Project grant. As the Department has grown, investigators have found these facilities to be increasingly essential to their research. The current core facilities are supported in part by the Department and other research grants. The continued financial support of these core facilities by the PPG is essential for the work proposed in this Program. Without these Cores, the research proposed in this application could not be conducted. The Research Services Core of this program has expanded in both scope and size during the previous grant period with the addition of new activities in Histology, Microscopy, and Image Processing. Eleven individuals currently staff the Research Sen/ices Core, each with significant experience in a broad range of activities that support the research infrastructure within the Department of Physiology. This staff is divided among one or more support groups, computer, analytical, animal monitoring, microscopy, machine and electronics, and instrument service. Based on the amount of crossover in the activities of these groups we have now found it more productive to unify their efforts into one core under the supervision of Dr. Andrews. Greene. Based on these natural divisions, the Research Services Core serves six main functions within the program project; computer support, analytical and statistical consultation, chronic animal monitoring, microscopy and image processing, custom machine and electronic fabrication, and instrument service. Each of these functions is served by a fully staffed facility which is available at all times to program investigators. Systems management is a key element in sustaining large and complex research efforts such as those supported by the staff of the Research Sen/ices Core. The concept is applied across all areas of the Research Services Core to maximize efficiency and flexibility in design and maintenance of research equipment and facilities used by the principal investigators. The ability to integrate the various component parts of a research protocol into a reproducible and manageable system is a result of combining experienced individuals of complementary skill sets to a given design problem. The objective is to provide a technical infrastructure forthe research programs from the laboratory bench to publication. Providing standard data acquisition systems, analysis programs, data management, and publication tools while extending and maintaining these systems to meet the research goals is the primary activity in this core. The Research Services Core continues to provide direct support to the Program Project Grant with computer hardware, software and networking as well as access to access to skilled machinists and electronic engineers capable of design and repair of specialized research equipmenL The facilities and skills available under one administrative and supervisory unit allow significant cost reductions for maintenance and repair of vital laboratory equipment. The integration of all facets of equipment management speeds repair time by efficient stocking of critical spare parts and effective use of outside specialized maintenance services and contracts. Providing a single point of contact for equipment acquisition and service allows Program Project investigators to benefit from purchase consolidation while enhancing vendor communication and evaluation. This also provides a managed repository for service manuals and user documentation for shared equipment Service histories and maintenance records provide cost benefit input for equipment life cycle management decisions. Computer services and analytical components have historically been an essential component of this Program Project and have always provided services to all of the Projects and Cores. The growth of this Core is a consequence of its place in the center of the integrative activities of this program, since it is literally the facility that provides the key link between the three Projects and the Genomics Core B serving as a vital and the most intensively used resource within the Program. In the previous funding period we have expanded our ability to do theoretical work by developing a close collaboration with the Biotechnology and Biomedical Engineering Center at MCW. This relationship, which fostered the mutual needs of both the Center and the Physiology Department, has been extremely successful. Under this model and in close collaboration the Bioinformatics Research Center within the HMGC, as well as the Division of Biostatistics, PPG investigators are provided with access to large scale, state-of-the-art computing and expertise in sophisticated data analysis and modeling. The machine and electronic core of the PPG provide investigators in the Program Project access to skilled machinists and electronic engineers capable of design and repair of specialized research equipmenL In the previous proposal, these activities were incorporated into the Research Services Core in order to provide all of the research support activities of the Program under one administrative and supervisory unit. The machine and electronic shops are heavily utilized by PPG investigators for both design and fabrication as well as repair of vital equipment in their laboratories. Because of the availability of skilled machinists and engineers within our program, we have been able to reduce our dependence on outside service organizations.