Macaque Resources

印度恒河猴患上一种与人类获得性免疫缺陷非常相似的疾病 感染猴免疫缺陷病毒(SIV)或嵌合猴-人类时的综合征(艾滋病) 免疫缺陷病毒(SIV)，代表了HIV感染的最佳动物模型。因为 由于猕猴和人类的免疫系统相似，临床前疫苗的开发工作进展缓慢 依赖于SIV和SHIV猕猴模型。尽管它们具有研究价值，但非人类 灵长类(NHP)是复杂的高级物种，需要专门的基础设施和专业知识 研究背景。NHP核心致力于巩固实验动物和细胞免疫学 艾滋病毒疫苗研究和设计(HIVRAD)计划项目的一部分，“艾滋病毒免疫规划” 通过疫苗接种广泛中和抗体的反应“成为由高度 经验丰富的专业技术人员和最先进的资源。NHP核心，正在运行 在俄勒冈州国家灵长类研究中心(ONPRC)内，将提供基础设施和专业 NHP实验方案和抗原(Ag)特异的CD4和CD8T的技术专长 细胞分析支持“艾滋病毒准种疫苗免疫原性和新城疫病毒挑战研究”。这个 具体目标是：1)提供全面、高效和安全的专门知识和基础设施 进行HIVRAD计划中与非人类灵长类动物相关的部分，包括动物选择， 研究方案实施和执行、样本获取和分发、动物护理以及 项目预算管理，以及2)向HIVRAD计划提供流式细胞仪检测Ag- 特异性T细胞免疫与SHIV免疫致病机制。非人的免疫监测部分 灵长类核心将提供SIV/HIV特异性T细胞免疫(多色细胞因子)的复杂分析 流式细胞术)和SHIV免疫致病(多染表型分析) 血液和组织中的动力学。 相关性(请参阅说明)： 一种安全有效的疫苗仍然是控制人类免疫缺陷病毒(HIV)的最大希望 大流行。这项建议中的研究重点是开发疫苗策略，以广泛推广 中和抗HIV抗体，这是针对HIV的宿主免疫反应的关键组成部分，可能会防止 感染。

The Indian rhesus macaque develops a disease that closely mimics human acquired immunodeficiency syndrome (AIDS) when infected by simian immunodeficiency virus (SIV) or chimeric simian-human immunodeficiency viruses (SHIV), and represents the best animal model for HIV infection. Because of the similarity of the immune systems of macaques and humans, preclinical vaccine development is heavily dependent on the SIV and SHIV macaque models. Their research value notwithstanding, nonhuman primates (NHPs) are complex, higher order species that require specialized infrastructure and expertise in a research setting. The NHP Core seeks to consolidate the experimental animal and cellular immunology portions of the HIV Vaccine Research and Design (HIVRAD) Program project, "Programming HIV Immune Response for Broadly Neutralizing Antibodies by Vaccination" into a single Core composed of highly experienced professional and technical personnel and state-of-the-art resources. The NHP Core, functioning within the Oregon National Primate Research Center (ONPRC), will provide infrastructure and professional and technical expertise for the NHP experimental protocol and the antigen (Ag)-specific CD4+ and CD8+ T cell analyses supporting the "HIV Quasispecies Vaccine Immunogenicity and SHIV Challenge Study". The Specific Aims are: 1) to provide specialized expertise and infrastructure for comprehensive, efficient and safe conduct of the nonhuman primate-related portions of the HIVRAD Program including animal selection, research protocol implementation and execution, sample acquisition and distribution, animal care, and project budget administration, and 2) to provide the HIVRAD Program with flow cytometric assays of Ag- specific T cell immunity and SHIV immunopathogenesis. The immune monitoring section of the Nonhuman Primate Core will provide sophisticated assays of SIV/HIV-specific T cell immunity (polychromatic cytokine flow cytometry) and SHIV immunopathogenesis (polychromatic phenotypic analysis) of SHIV target cell dynamics in blood and tissue. RELEVANCE (See instructions): A safe and effective vaccine remains the best hope of controlling the human immunodeficiency virus (HIV) pandemic. The studies in this proposal focus on developing vaccine strategies to promote broadly neutralizing anti-HIV antibody, a key component of the host immune response against HIV that may prevent infection.