Time-keeping mechanisms in Drosophila embryonic development

果蝇胚胎发育的计时机制

• 批准号: 8424355
• 负责人: Stefano Di Talia
• 金额: $ 9.69万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-05-20 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8424355
• 关键词: Address; Affect; Award; Biochemical; Biological; Biological Sciences; Cell Cycle Regulation; Cell Fate Control; Cell division; Cells; Cellular biology; Congenital Abnormality; Controlled Study; DNA biosynthesis; Development; Developmental Biology; Doctor of Philosophy; Drosophila genus; Educational process of instructing; Embryo; Embryology; Embryonic Development; Enhancers; Ensure; Feedback; Fellowship; Gene Expression; Genetic; Genetic Screening; Genetic Techniques; Genetic Transcription; Image; Laboratories; Life; Marines; Measures; Mitosis; Molecular; Molecular Biology; Molecular Genetics; Noise; Nuclear; Organism; Pathway interactions; Phase; Regulation; Regulator Genes; Reproducibility; Research; Role; Schedule; Signal Transduction; Signaling Protein; System; Techniques; Testing; Time; Training; United States National Institutes of Health; Universities; career; cell behavior; gastrulation; genetic regulatory protein; insight; mathematical model; mutant; novel; photo switch; professor; programs; protein degradation; public health relevance; research study; response; skills

DESCRIPTION (provided by applicant): The candidate is currently a Life Science Research Fellow in the laboratory of Professor Eric Wieschaus in the Department of Molecular Biology at Princeton University. The candidate was awarded a PhD from The Rockefeller University for research in quantitative cell biology. During the Postdoctoral Fellowship, the candidate is transitioning to the field of developmental biology. The candidate will apply the quantitative and analytical techniques from previous research as well as further training in genetics and developmental biology to the study of the control of timing of cell behaviors during embryonic development. The NIH Pathway to Independence Award would provide necessary support to the candidate during this transition period. The award would allow the candidate to acquire new skills in genetics and developmental biology as well as to establish novel research directions. The candidate will benefit from the opportunity to take the graduate course 'Genetics of Multicellular Organisms' at Princeton University as well as the courses 'Eukaryotic Gene Expression' and 'Gene Regulatory Networks for Development' at Cold Spring Harbor Laboratory and at the Marine Biological Laboratory respectively. The candidate will study the molecular mechanisms ensuring precise temporal regulation of cell division through control of gene expression, signaling and protein degradation during Drosophila embryonic development. During the K99 phase of the award, the candidate will 1) Develop theoretical analysis of the integration time of signaling systems 2) Perform genetic screens and molecular biology experiments to identify regulators of Cdc25 transcription (rate-limiting activator of the cell cycl) 3) Determine the importance of regulation of Cdc25 protein degradation at the maternal-to-zygotic transition, a critical developmental transition. These aims will be accomplished by combining genetics, embryology, molecular biology, quantitative live imaging and mathematical modeling. During the R00 phase of the award, the candidate will extend the research by determining the molecular mechanisms ensuring that Cdc25 is transcribed and degraded with high temporal precision and by analyzing signaling systems beyond cell cycle control. The candidate ultimately desires to pursue an academic career in research and teaching.

描述（由申请人提供）：该候选人目前是普林斯顿大学分子生物学系 Eric Wieschaus 教授实验室的生命科学研究人员。该候选人因定量细胞生物学研究而获得洛克菲勒大学博士学位。在博士后奖学金期间，候选人正在过渡到发育生物学领域。候选人将应用先前研究的定量和分析技术以及遗传学和发育生物学的进一步培训来研究胚胎发育过程中细胞行为时间的控制。 NIH 独立之路奖将在此过渡期间为候选人提供必要的支持。该奖项将使候选人能够获得遗传学和发育生物学方面的新技能，并建立新的研究方向。候选人将受益于在普林斯顿大学修读研究生课程“多细胞生物遗传学”以及分别在冷泉港实验室和海洋生物实验室修读“真核基因表达”和“发育基因调控网络”课程的机会。候选人将研究分子机制，通过控制果蝇胚胎发育过程中的基因表达、信号传导和蛋白质降解，确保细胞分裂的精确时间调节。在该奖项的 K99 阶段，候选人将 1) 对信号系统整合时间进行理论分析 2) 进行遗传筛选和分子生物学实验，以确定 Cdc25 转录调节因子（细胞周期的限速激活因子） 3) 确定 Cdc25 蛋白降解调节在母体到合子转变（一个关键的发育转变）中的重要性。这些目标将通过结合遗传学、胚胎学、分子生物学、定量实时成像和数学建模来实现。在该奖项的 R00 阶段，候选人将通过确定确保 Cdc25 以高时间精度转录和降解的分子机制以及分析细胞周期控制之外的信号系统来扩展研究。候选人最终希望从事研究和教学方面的学术职业。