Investigations into ASIC1a-dependent neuronal death

ASIC1a 依赖性神经元死亡的研究

• 批准号: 10393671
• 负责人: CANDICE C ASKWITH
• 金额: $ 36.47万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-15 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10393671
• 关键词: ASIC channel; Acidosis; Acids; Agonist; Biochemical; Biological Assay; Blood flow; Brain; Brain Injuries; Brain Ischemia; Cell Death; Cell Death Induction; Cell Line; Cerebrum; Cessation of life; Clinical; Co-Immunoprecipitations; Collaborations; Data; Disease; Hour; In Vitro; Individual; Intervention; Investigation; Ion Channel; Ions; Ischemia; Ischemic Brain Injury; Ischemic Stroke; Knockout Mice; Label; Measurement; Measures; Mediating; Methods; Middle Cerebral Artery Occlusion; Modeling; Modification; Molecular Profiling; Multiple Sclerosis; Mus; Mutation; Nervous System Trauma; Neurodegenerative Disorders; Neurons; Opioid agonist; Outcome; Pathologic; Phosphorylation; Phosphorylation Site; Physiological; Physiology; Play; Preparation; Prevention; Proteins; Receptor Activation; Research Personnel; Retinal Degeneration; Role; Signal Pathway; Signal Transduction; Slice; Spinocerebellar Ataxias; Testing; Tissues; Toxic effect; Traumatic Brain Injury; Work; delta opioid receptor; extracellular; in vivo; injury prevention; ischemic injury; neuron loss; neuroprotection; new therapeutic target; novel; pharmacologic; prevent; protein protein interaction

The acid sensing ion channel1a (ASIC1a) is essential for normal brain function, but initiates neuronal death and contributes to ischemic brain injury. Prolonged reductions in extracellular pH accompany ischemia and ASIC1a inhibition limits neurological damage. Yet, ASICs also play an important role in normal physiology and established models of ASIC-induced cell death make it difficult to develop strategies that specifically inhibit ASIC1a toxicity. Our preliminary data support a newer model of ASIC1a-induced cell death. Specifically, we have discovered that the toxic effect of ASIC1a can be eliminated by modification of the intracellular region of the channel or activation of the delta opioid receptor (DOR). An especially provocative aspect of these findings is that acidotoxicity is inhibited without a reduction in ASIC1a current, thereby suggesting that the toxic and physiological actions of the channel can be separated. Our central hypothesis is that DOR prevents acidotoxicity through signaling cascades, which act on the intracellular domain of ASIC1a to limit protein interactions required for toxicity. To test this hypothesis, we will define the mechanisms governing DOR action on ASIC1a and elucidate their role in ischemic injury in vivo. The outcomes of the proposed work will reveal novel regulatory mechanisms controlling ASIC1a-induced toxicity, suggest new interventions to mitigate ASIC-induced death using existing DOR agonists, and reveal strategies to separate the physiological and pathological actions of ASIC1a. These results will be significant as they are expected to have broad implications for the prevention of brain injury following ischemic stroke as well as other disorders where neuronal acidotoxicity plays a role.

酸感应离子通道1a （ASIC1a）对正常脑功能至关重要，但会引发神经元死亡

项目成果

In vivo Mouse Intervertebral Disc Degeneration Models and Their Utility as Translational Models of Clinical Discogenic Back Pain: A Comparative Review.

• DOI: 10.3389/fpain.2022.894651
• 发表时间: 2022
• 期刊: Frontiers in pain research (Lausanne, Switzerland)

Sleep fragmentation engages stress-responsive circuitry, enhances inflammation and compromises hippocampal function following traumatic brain injury.

• DOI: 10.1016/j.expneurol.2022.114058
• 发表时间: 2022-07
• 期刊: EXPERIMENTAL NEUROLOGY
• 影响因子: 5.3
• 作者: Tapp, Zoe M.;Cornelius, Sydney;Oberster, Alexa;Kumar, Julia E.;Atluri, Ravitej;Witcher, Kristina G.;Oliver, Braedan;Bray, Chelsea;Velasquez, John;Zhao, Fangli;Peng, Juan;Sheridan, John;Askwith, Candice;Godbout, Jonathan P.;Kokiko-Cochran, Olga N.
• 通讯作者: Kokiko-Cochran, Olga N.