Pilot 6 - Developmental Exposure Alcohol Research Center

我们最近利用了一种新的酒精摄入量有限的模型， 在整个妊娠期间，C57 BL 6/J妊娠小鼠每天暴露于乙醇2小时。母亲每日乙醇摄入量 5-6 g/kg的剂量在成年和青少年后代中产生许多行为缺陷。特别是催眠药 对乙醇的敏感性在出生后第30天和第90天降低（P<0.01），这可能表明产前 乙醇暴露改变了正常发育的GABAA受体系统。本提案的目标是 (1)确定母体DID是否改变催眠药和GABAA受体对几种药物的敏感性， （2）检查青少年中各种GABAA受体亚单位的表达， 这些老鼠的成年后代

We have recently taken advantage of a new limited access model of binge-like ethanol intake to expose pregnant C57BL6/J mice to ethanol for 2 hours each day throughout gestation. Daily maternal ethanol intakes of 5-6 g/kg produced a number of behavioral deficits in adult and adolescent offspring. In particular, hypnotic sensitivity to ethanol was reduced at postnatal day (P) 30 and P90 (p<0.01) possibly suggesting that prenatal ethanol exposure alters the normal development GABAA receptor systems. The goals of this proposal are to (1) determine whether maternal DID alters hypnotic and GABAA receptor sensitivity to several drugs with actions at the receptor and (2) to examine the expression of various GABAA receptor subunits in the adolescent and adult offspring of these mice.