Real-time Internal Calibration for Multiplexed Microarray Analysis

描述（由申请人提供）：对于用于多重标记物分析的高效、简单且易于使用的定量方法的需求日益增长。我们假设，在异质阵列为基础的平台，不同地址的分析物通量的差异可以作为一种有效的手段，同时确定的绝对浓度的分析物的数量，只需要添加一个单一的校准样品。这项建议详细说明了减少技术的做法，这将提供一个简单和具有成本效益的办法 以确定每种标记物的浓度，而不考虑标记物的数量，而不需要创建校准曲线或使用校准物。该平台是可扩展的- 这意味着它可以用于基因组和代谢研究以及平台蛋白质组学分析，并可以应用于其他读出方法。

DESCRIPTION (provided by applicant): There is a growing need for a highly efficient, simple, and easy to use quantitation method for multiplexed marker analyses. We hypothesize that differences in analyte flux to different addresses in a heterogeneous array-based platform can serve as an effective means to simultaneously determine the absolute concentration of a number of analytes with only the need to add a single calibrant to the sample. This proposal details a reduction to practice of technology that will afford a simple and cost-effective approach to determine the concentration of each marker - irrespective of the number of markers - without requiring the creation of calibration curves or the use of calibrants. The platform is extensible - meaning it could be deployed for genomic and metabolic studies as well as for platform proteomic assays - and could be applied to other readout methods.