Bioactivity of NLP peptides of Ascaris suum

猪蛔虫NLP肽的生物活性

• 批准号: 8430302
• 负责人: ANTONY O. STRETTON
• 金额: $ 16.92万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8430302
• 关键词: Acetylcholine; Adult; Amino Acids; Anterior; Aplysia; Ascaris; Ascaris lumbricoides; Ascaris suum; Attention; Base Sequence; Behavior; Biological; C-terminal; Cells; Chemicals; Childhood; Databases; Development; Dissection; Dose; Drug Administration Routes; EST Library; FMRFamide; Family; Ganglia; Genes; Genome; Goals; Health; Human; In Situ Hybridization; Individual; Intestines; Length; Mass Spectrum Analysis; Measures; Mediating; Membrane Potentials; Methods; Mining; Motor; Muscle; Muscle Cells; Muscle Contraction; Nematoda; Nerve; Nervous system structure; Neurons; Neuropeptides; Neurotransmitters; Nucleic acid sequencing; Open Reading Frames; Parasites; Parasitic nematode; Penetration; Peptides; Pharmaceutical Preparations; Physiological; Play; Post-Translational Protein Processing; Process; Productivity; Protein Precursors; Proteolysis; Relative (related person); Research; Resistance; Role; Series; Signal Transduction; Site; Synaptic Potentials; Techniques; Tissues; Translating; Variant; high risk; interest; mortality; myomodulin; neuromuscular transmission; neuronal cell body; public health relevance; receptor; research study; response

DESCRIPTION (provided by applicant): The research described in this proposal aims at determining the biological role of a family of 8 related endogenous neuropeptides, encoded by 4 different genes in A. suum. The peptide encoded by one gene has been shown to have very potent inhibitory activity on Ascaris suum muscle. We propose to characterize the biological activity of the remaining 7 peptides. We will also identify the cells (identified neurons, or non-neural cells) which make each peptide, and the site of the receptors for each peptide. We will also study the mechanism of action of each peptide by electrophysiological methods. There are preliminary indications that at least one of these peptides may be expressed in the intestine. We will thoroughly explore this hypothesis, since it suggests a potential new target for anti-nematode drugs, and, even more interesting, a different route for administration of drugs that would not necessitate penetration into the interior tissue space of the nematode.

描述（由申请人提供）：本提案中描述的研究旨在确定由4个不同基因编码的8个相关内源性神经肽家族的生物学作用。由一个基因编码的肽已被证明对猪蛔虫肌肉有很强的抑制活性。我们建议表征其余7个肽的生物活性。我们还将识别制造每种肽的细胞（已识别的神经元或非神经细胞），以及每种肽的受体位置。我们还将通过电生理方法研究各肽的作用机制。有初步迹象表明，这些肽中至少有一种可能在肠道中表达。我们将彻底探索这一假设，因为它提出了抗线虫药物的潜在新靶点，更有趣的是，这是一种不需要渗透到线虫内部组织空间的药物给药的不同途径。