Bioactivity of NLP peptides of Ascaris suum

猪蛔虫NLP肽的生物活性

基本信息

批准号：
8430302
负责人：
ANTONY O. STRETTON
金额：
$ 16.92万
依托单位：
UNIVERSITY OF WISCONSIN-MADISON
依托单位国家：
美国
项目类别：
财政年份：
2013
资助国家：
美国
起止时间：
2013-06-01 至 2015-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8430302
关键词：
Acetylcholine Adult Amino Acids Anterior Aplysia Ascaris Ascaris lumbricoides Ascaris suum Attention Base Sequence Behavior Biological C-terminal Cells Chemicals Childhood Databases Development Dissection Dose Drug Administration Routes EST Library FMRFamide Family Ganglia Genes Genome Goals Health Human In Situ Hybridization Individual Intestines Length Mass Spectrum Analysis Measures Mediating Membrane Potentials Methods Mining Motor Muscle Muscle Cells Muscle Contraction Nematoda Nerve Nervous system structure Neurons Neuropeptides Neurotransmitters Nucleic acid sequencing Open Reading Frames Parasites Parasitic nematode Penetration Peptides Pharmaceutical Preparations Physiological Play Post-Translational Protein Processing Process Productivity Protein Precursors Proteolysis Relative (related person)Research Resistance Role Series Signal Transduction Site Synaptic Potentials Techniques Tissues Translating Variant high risk interest mortality myomodulin neuromuscular transmission neuronal cell body public health relevance receptor research study response

项目摘要

DESCRIPTION (provided by applicant): The research described in this proposal aims at determining the biological role of a family of 8 related endogenous neuropeptides, encoded by 4 different genes in A. suum. The peptide encoded by one gene has been shown to have very potent inhibitory activity on Ascaris suum muscle. We propose to characterize the biological activity of the remaining 7 peptides. We will also identify the cells (identified neurons, or non-neural cells) which make each peptide, and the site of the receptors for each peptide. We will also study the mechanism of action of each peptide by electrophysiological methods. There are preliminary indications that at least one of these peptides may be expressed in the intestine. We will thoroughly explore this hypothesis, since it suggests a potential new target for anti-nematode drugs, and, even more interesting, a different route for administration of drugs that would not necessitate penetration into the interior tissue space of the nematode.

描述（由申请人提供）：本提案中描述的研究旨在确定8个相关内源性神经肽家族的生物学作用，该家族由A. suum中的4个不同基因编码。通过一个基因编码的肽已被证明对Ascaris suum肌肉具有非常有效的抑制活性。我们建议表征其余7种肽的生物学活性。我们还将确定使每种肽的细胞（已鉴定的神经元或非神经细胞）和每个肽的受体位点。我们还将通过电生理方法研究每种肽的作用机理。有初步的迹象表明，这些肽中至少可以在肠道中表达。我们将彻底探讨这一假设，因为它暗示了抗新移虫药物的潜在新靶标，甚至更有趣的是，不需要穿透线虫的内部组织空间的药物的不同途径。