Structure and Function of Ascaris Neuropeptides
蛔虫神经肽的结构和功能
基本信息
- 批准号:7759587
- 负责人:
- 金额:$ 33.71万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2006
- 资助国家:美国
- 起止时间:2006-02-15 至 2012-01-31
- 项目状态:已结题
- 来源:
- 关键词:AffectAffinityAntibodiesAreaAscarisAscaris suumBasic ScienceBehaviorBiochemicalCaenorhabditis elegansCellsDorsalDrug resistanceFamilyGangliaGenerationsGeneticGrantHigh Pressure Liquid ChromatographyIn Situ HybridizationIndiumIndividualInterneuronsLasersLocomotionMALDI-TOF Mass SpectrometryMass Spectrum AnalysisMediatingMethodsMolecularMolecular BiologyMorphologyMotorMotor ActivityMotor NeuronsMovementMuscleMuscle CellsMuscle TensionMyxoid cystNematodaNerveNerve TissueNervous system structureNeuronsNeuropeptide GeneNeuropeptidesParalysedParasitic nematodePeptidesPharmaceutical PreparationsPhysiologicalPlayPositioning AttributeProcessRelaxationReportingResearchRoleSamplingSignal TransductionSiteSourceStructureSystemTechniquesTestingTimeTissuesTranscriptWorkbasecellular targetingdesigner antibodyfallsimmunocytochemistryin vivointerdisciplinary approachmolecular massneuromuscular systemneuronal cell bodynovelprotein aminoacid sequencerelating to nervous systemresearch study
项目摘要
The locomotory system of nematodes is the target of many currently used anthelminthics, since
disruption of locomotory behavior interferes with the normal processes by which many nematodes maintain
their position within the host. The problem of genetic drug resistance is making many of the available drugs
less effective, so it is important to develop new generations of anti-nematdde drugs. The proposed research
aims to describe new intercellular signaling systems that are important in the motornervous system that
controls locomotion. Our strategy is based on our previous work in which we isolated and sequenced a large
family of 29 novel endogenous neuropeptides in the parasitic nematode, Ascaris suum. Preliminary
experiments have shown that almost all of the peptides tested have potent activity .on the neuromuscular
system of Ascaris. Some cause paralysis, others exaggerate locomotory movements. Neuropeptides,
therefore, play an important role in the overall activity of the motornervous system. There is every indication
that there are many more peptides than the ones we have isolated so far. There is no indication that the
currently available peptides are the most potent, so we propose to isolate new peptides, concentrating on
those that are present in the motornervous system, in order to detect new peptides that have potent
physiological activity. This is basic research upon which new generations of anthelminthics can ultimately be
developed.
线虫的运动系统是许多目前使用的驱虫剂的靶标,因为
运动行为的中断干扰了许多线虫维持生命的正常过程,
在宿主中的位置。基因抗药性的问题使得许多可用的药物
因此,开发新一代的抗抑郁药物非常重要。拟议研究
目的是描述新的细胞间信号系统是重要的运动神经系统,
控制运动我们的策略是基于我们以前的工作,我们分离和测序了一个大的
猪蛔虫中29种新的内源性神经肽家族。初步
实验表明,几乎所有测试的肽对神经肌肉
蛔虫系统有些会导致瘫痪,有些则会夸大运动。神经肽,
因此,在运动神经系统的整体活动中发挥重要作用。种种迹象
比我们目前分离出的肽要多得多。没有迹象表明
目前可用的肽是最有效的,所以我们建议分离新的肽,集中在
那些存在于运动神经系统中的肽,以检测具有强效活性的新肽
生理活动这是新一代驱虫药最终可以
开发
项目成果
期刊论文数量(25)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Changes in cyclic nucleotides, locomotory behavior, and body length produced by novel endogenous neuropeptides in the parasitic nematode Ascaris suum.
寄生线虫猪蛔虫中新型内源神经肽产生的环核苷酸、运动行为和体长的变化。
- DOI:10.1016/j.molbiopara.2011.08.001
- 发表时间:2011
- 期刊:
- 影响因子:1.5
- 作者:Reinitz,CatharineA;Pleva,AnthonyE;Stretton,AntonyOW
- 通讯作者:Stretton,AntonyOW
The effects of avermectin and drugs related to acetylcholine and 4-aminobutyric acid on neurotransmission in Ascaris suum.
阿维菌素及乙酰胆碱、4-氨基丁酸相关药物对猪蛔虫神经传递的影响。
- DOI:10.1016/0166-6851(84)90114-2
- 发表时间:1984
- 期刊:
- 影响因子:1.5
- 作者:Kass,IS;Stretton,AO;Wang,CC
- 通讯作者:Wang,CC
Excitatory and inhibitory activity in the dorsal musculature of the nematode Ascaris evoked by single dorsal excitatory motonerons.
由单个背侧兴奋性运动元引起的线虫蛔虫背侧肌肉组织的兴奋性和抑制活性。
- DOI:10.1523/jneurosci.05-01-00016.1985
- 发表时间:1985
- 期刊:
- 影响因子:0
- 作者:Walrond,JP;Stretton,AO
- 通讯作者:Stretton,AO
Actions of cholinergic drugs in the nematode Ascaris suum. Complex pharmacology of muscle and motorneurons.
- DOI:10.1085/jgp.101.2.271
- 发表时间:1993-02
- 期刊:
- 影响因子:0
- 作者:Segerberg MA;Stretton AO
- 通讯作者:Stretton AO
De novo sequencing of novel neuropeptides directly from Ascaris suum tissue using matrix-assisted laser desorption/ionization time-of-flight/time-of-flight.
使用基质辅助激光解吸/电离飞行时间/飞行时间直接从猪蛔虫组织中对新型神经肽进行从头测序。
- DOI:10.1002/rcm.1240
- 发表时间:2003
- 期刊:
- 影响因子:0
- 作者:Yew,JoanneY;Dikler,Sergei;Stretton,AntonyO
- 通讯作者:Stretton,AntonyO
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ANTONY O. STRETTON其他文献
ANTONY O. STRETTON的其他文献
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