ANTIBODIES TO NEURAL ANTIGENS IN NEMATODE ASCARIS

蛔虫线虫神经抗原抗体

• 批准号: 3129970
• 负责人: ANTONY O. STRETTON
• 金额: $ 10.35万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-07-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3129970
• 关键词: antibody specificity; choline acetyltransferase; glutamate decarboxylase; helminthic antigen; hybridomas; immunochemistry; monoclonal antibody; neural information processing; neuroanatomy; neurophysiology; synapses; tissue /cell culture

Our aim is to devise immunohistochemical labels for neurons and synapses in the motornervous system of the large parasitic nematode Ascaris. We will generate and use antibodies against synapses and against neurotransmitters and the enzymes responsible for their synthesis. Since we have electrophysiological and anatomical information about many of the neurons in the motor nervous system, we can correlate the presence of specific antigens with known physiological activities. Our objective is to generate a series of labels so that the function of a neuron can be determined using histochemical techniques that label the molecules that mediate function. This will enable us to determine the functional anatomy of the entire nematode nervous system in the detail that will be required before we can test our understanding of the cellular and molecular mechanisms that the nervous system uses to process information. Gaining a basic understanding of the Ascaris nervous system is important since Ascaris is a model nematode in which to investigate the control of locomotion in nematodes. The Ascaris motor nervous system is anatomically similar to that of other nematodes, so it is reasonable to assume that this similarity will also apply at the functional level. By studying the mechanisms underlying the activity of the neuromuscular system of Ascaris, general principles applying to other nematodes will be found. This basic understanding of the nematode nervous system will make a contribution to parasite biology, and allow differences in the biology between host and parasite to be identified as targets for anthelmintic drugs. In view of the agricultural impact of nematodes on food supplies, and the problems they cause as animal and human parasites, especially in tropical and economically less-developed countries, this is an important health-related goal.

我们的目标是设计出神经元和突触的免疫组织化学标记。 大型寄生线虫的运动神经系统。我们会 产生和使用针对突触和神经递质的抗体 以及负责它们合成的酶。因为我们有 关于许多神经元的电生理和解剖学信息 在运动神经系统中，我们可以关联到特定的 具有已知生理活性的抗原。我们的目标是产生 用来确定神经元功能的一系列标记 标记调节功能的分子的组织化学技术。 这将使我们能够确定整个人体的功能解剖 在了解线虫神经系统的细节之前，我们将需要 测试我们对细胞和分子机制的理解 神经系统用来处理信息。 对蛔虫神经系统有一个基本的了解很重要 由于蛔虫是一种模式线虫，可以在其中研究控制 线虫的运动。蛔虫的运动神经系统是解剖学上的 与其他线虫相似，所以有理由认为这是 相似性也将适用于职能层面。通过研究 蛔虫神经肌肉系统活动的潜在机制， 适用于其他线虫的一般原理将会被发现。这是基本的 对线虫神经系统的了解将有助于 寄生生物学，并允许宿主和寄主之间的生物学差异 寄生虫将被确定为驱虫药的靶标。鉴于…… 农业线虫对粮食供应的影响及存在的问题 它们作为动物和人类的寄生虫引起，特别是在热带和 在经济欠发达国家，这是一个重要的与健康相关的问题 进球。