Title: Role of Heparan sulfate in B-cell responses

标题：硫酸乙酰肝素在 B 细胞反应中的作用

• 批准号: 8501350
• 负责人: LAURENT COSCOY
• 金额: $ 16.5万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2014-12-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8501350
• 关键词: Adhesions; Affect; Affinity; Antibodies; Antibody Formation; Antigens; Antiviral Response; Autoimmunity; B-Lymphocytes; Binding; Cell Adhesion; Cell physiology; Cell surface; Cells; Complex; Data; Development; Ensure; Excision; Extracellular Matrix; Growth Factor; Hen Egg Lysozyme; Heparitin Sulfate; Herpesviridae Infections; Immune response; Immune system; Infection; Influenza; Interferons; Ligands; Modeling; Modification; Mus; Play; Poly C; Reporting; Role; Series; Signal Transduction; Surface; T-Lymphocyte; Testing; Up-Regulation; Vaccines; Virus; Virus Diseases; Wild Type Mouse; Work; antigen binding; cell motility; chemokine; cytokine; extracellular; gammaherpesvirus; in vivo; pathogen; prevent; public health relevance; receptor; research study; response; sugar; uptake; vaccine development; vaccinology

DESCRIPTION (provided by applicant): Heparan sulfate (HS) influences many cellular processes including adhesion, motility, ligand-receptor interaction, and proliferation. We have recently observed that the expression of HS was tightly regulated on B-cells: whereas naive B-cells do not express heparan sulfate, all B-cells isolated from mice infected with viruses, or mice injected with poly I:C, express heparan sulfate. Accordingly, B-cells treated ex-vivo with IFN-I alone express high level of heparan sulfate. Our preliminary data indicates that HS expression on B-cells regulates their responsiveness to cytokines as well as their motility in vivo. Here we propose experiments aimed at validating the importance of HS for mounting of an efficient antiviral response. This work might help in the understanding of B-cell responses and has strong implication for vaccine development and autoimmunity.

描述（由申请方提供）：硫酸乙酰肝素（HS）影响许多细胞过程，包括粘附、运动、配体-受体相互作用和增殖。我们最近观察到，HS的表达在B细胞上受到严格的调节：而幼稚B细胞不表达硫酸乙酰肝素，所有从病毒感染的小鼠或小鼠中分离的B细胞， 注射聚I：C，表达硫酸乙酰肝素。因此，单独用IFN-I离体处理的B细胞表达高水平的硫酸乙酰肝素。我们的初步数据表明，B细胞上的HS表达调节其对细胞因子的反应性以及体内运动性。在这里，我们提出的实验，旨在验证HS安装一个有效的抗病毒反应的重要性。这项工作可能有助于了解B细胞反应，并对疫苗开发和自身免疫具有重要意义。

项目成果

Virally-induced upregulation of heparan sulfate on B cells via the action of type I IFN.

• DOI: 10.4049/jimmunol.1003495
• 发表时间: 2011-12-01
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Jarousse N;Trujillo DL;Wilcox-Adelman S;Coscoy L
• 通讯作者: Coscoy L